Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSN › Conference contribution/Paper › peer-review
Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSN › Conference contribution/Paper › peer-review
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TY - GEN
T1 - Preparation and investigation of nano-carbonated hydroxy apatite/polymer composite spongy scaffolds for biomedical applications
AU - Siddiqi, S.A.
AU - Manzoor, F.
AU - Azhar, U.
AU - Jamal, A.
AU - Tariq, M.
AU - Anwar, M.S.
AU - Saleem, M.
AU - Chaudhry, A.A.
AU - Rehman, I.U.
A2 - M., Laudon
A2 - B., Romanowicz
PY - 2015
Y1 - 2015
N2 - Spongy scaffolds containing nano-carbonatcd hydroxyapatitc (nCHA), with composition Ca10(P04)6- y(C03)y(0H)2,with y=2,4, were prepared by the Iyophilization method. Different polymers, natural gelatin (G), chitosan (CS) and polyvinyl alcohol (PVA), alone or in combination, were used to make their compositcs with nCHA. All the samples were characterized by (XRD), (SEM), (FT-IR) techniques. P3rticlc size and surface area were determined by BET surfacc area analysis. The nCHA containing scaffolds mediated cytotoxicity (MTT assay) and cell attachment studies (SEM) were carried out on rat bone marrow derived mesenchymal stem cell (MSC). SEM studies revealed varying microstmctures for pore sizes, shapes and pore interconnectivicy. All the scaffolds were found to be highly porous, porosities beyond 70%. It is observed that the scafTold morphology changes significantly for one nCHA-'polymer combination to the other. Furthermore we investigated the biocompatibility of these scaffolds by performing cell viability and cell attachment tests. Our results indicated evident cell attachment on all the materials and also no significant cytotoxicity was observed in cells grown with these materials compared with control. The preparation of spongy scaffolds and their biocompatibility augment their potential use in tissue engineering and biomedical applications. © 2015 by TechConnect. All rights reserved.
AB - Spongy scaffolds containing nano-carbonatcd hydroxyapatitc (nCHA), with composition Ca10(P04)6- y(C03)y(0H)2,with y=2,4, were prepared by the Iyophilization method. Different polymers, natural gelatin (G), chitosan (CS) and polyvinyl alcohol (PVA), alone or in combination, were used to make their compositcs with nCHA. All the samples were characterized by (XRD), (SEM), (FT-IR) techniques. P3rticlc size and surface area were determined by BET surfacc area analysis. The nCHA containing scaffolds mediated cytotoxicity (MTT assay) and cell attachment studies (SEM) were carried out on rat bone marrow derived mesenchymal stem cell (MSC). SEM studies revealed varying microstmctures for pore sizes, shapes and pore interconnectivicy. All the scaffolds were found to be highly porous, porosities beyond 70%. It is observed that the scafTold morphology changes significantly for one nCHA-'polymer combination to the other. Furthermore we investigated the biocompatibility of these scaffolds by performing cell viability and cell attachment tests. Our results indicated evident cell attachment on all the materials and also no significant cytotoxicity was observed in cells grown with these materials compared with control. The preparation of spongy scaffolds and their biocompatibility augment their potential use in tissue engineering and biomedical applications. © 2015 by TechConnect. All rights reserved.
KW - Carbonated hydroxyapatue
KW - Cell attachment
KW - Freeze drying
KW - Lyophilization
KW - Polymer scaffolds
KW - Spongy scaffolds
KW - Biocompatibility
KW - Cell culture
KW - Cells
KW - Cytology
KW - Medical applications
KW - Natural polymers
KW - Phosphate minerals
KW - Polymers
KW - Pore size
KW - Stem cells
KW - Tissue engineering
KW - Cell attachments
KW - Scaffolds (biology)
M3 - Conference contribution/Paper
SN - 9781498747295
SP - 270
EP - 273
BT - TechConnect World Innovation Conference & Expo 2015
PB - TechConnect
ER -