Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Protein destabilisation by ruthenium(II) tris-bipyridine based protein-surface mimetics
AU - Wilson, Andrew J.
AU - Ault, James R.
AU - Filby, Maria H.
AU - Philips, Hazel I. A.
AU - Ashcroft, Alison E.
AU - Fletcher, Nicholas C.
PY - 2013/4/7
Y1 - 2013/4/7
N2 - Highly functionalised ruthenium(II) tris-bipyridine receptor 1 which acts as a selective sensor for equine cytochrome c (cyt c) is shown to destabilise the native protein conformation by around 25 degrees C. Receptors 2 and 3 do not exert this effect confirming the behaviour is a specific effect of molecular recognition between 1 and cyt c, whilst the absence of a destabilising effect on 60% acetylated cyt c demonstrates the behaviour of 1 to be protein specific. Molecular recognition also modifies the conformational properties of the target protein at room temperature as evidenced by ion-mobility spectrometry (IMS) and accelerated trypsin proteolysis.
AB - Highly functionalised ruthenium(II) tris-bipyridine receptor 1 which acts as a selective sensor for equine cytochrome c (cyt c) is shown to destabilise the native protein conformation by around 25 degrees C. Receptors 2 and 3 do not exert this effect confirming the behaviour is a specific effect of molecular recognition between 1 and cyt c, whilst the absence of a destabilising effect on 60% acetylated cyt c demonstrates the behaviour of 1 to be protein specific. Molecular recognition also modifies the conformational properties of the target protein at room temperature as evidenced by ion-mobility spectrometry (IMS) and accelerated trypsin proteolysis.
KW - SPECTROMETRY-MASS SPECTROMETRY
KW - CYTOCHROME-C
KW - FLUORESCENT PROTEIN
KW - DESIGNED MOLECULES
KW - DRUG DISCOVERY
KW - RECOGNITION
KW - RECEPTORS
KW - BINDING
KW - COMPLEXES
KW - PEPTIDE
U2 - 10.1039/c3ob26251k
DO - 10.1039/c3ob26251k
M3 - Journal article
VL - 11
SP - 2206
EP - 2212
JO - Organic and Biomolecular Chemistry
JF - Organic and Biomolecular Chemistry
SN - 1477-0520
IS - 13
ER -