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Protein-small molecule interactions in neocarzinostatin, the prototypical enediyne chromoprotein antibiotic

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Protein-small molecule interactions in neocarzinostatin, the prototypical enediyne chromoprotein antibiotic. / Baker, James R.; Woolfson, Derek N.; Muskett, Frederick W. et al.
In: ChemBioChem, Vol. 8, No. 7, 07.05.2007, p. 704-717.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Baker, JR, Woolfson, DN, Muskett, FW, Stoneman, RG, Urbaniak, MD & Caddick, S 2007, 'Protein-small molecule interactions in neocarzinostatin, the prototypical enediyne chromoprotein antibiotic', ChemBioChem, vol. 8, no. 7, pp. 704-717. https://doi.org/10.1002/cbic.200600534

APA

Baker, J. R., Woolfson, D. N., Muskett, F. W., Stoneman, R. G., Urbaniak, M. D., & Caddick, S. (2007). Protein-small molecule interactions in neocarzinostatin, the prototypical enediyne chromoprotein antibiotic. ChemBioChem, 8(7), 704-717. https://doi.org/10.1002/cbic.200600534

Vancouver

Baker JR, Woolfson DN, Muskett FW, Stoneman RG, Urbaniak MD, Caddick S. Protein-small molecule interactions in neocarzinostatin, the prototypical enediyne chromoprotein antibiotic. ChemBioChem. 2007 May 7;8(7):704-717. doi: 10.1002/cbic.200600534

Author

Baker, James R. ; Woolfson, Derek N. ; Muskett, Frederick W. et al. / Protein-small molecule interactions in neocarzinostatin, the prototypical enediyne chromoprotein antibiotic. In: ChemBioChem. 2007 ; Vol. 8, No. 7. pp. 704-717.

Bibtex

@article{d5903c0a08df49a4830332a9cc4b0997,
title = "Protein-small molecule interactions in neocarzinostatin, the prototypical enediyne chromoprotein antibiotic",
abstract = "The enediyne chromoproteins are a class of potent antitumour antibiotics comprising a 1:1 complex of a protein and a noncovalently bound chromophore. The protein is required to protect and transport the highly labile chromophore, which acts as the cytotoxic component by reacting with DNA leading to strand cleavage. A derivative of the best-studied member of this class, neocarzinostatin (NCS), is currently in use as a chemotherapeutic in Japan. The application of the chromoproteins as therapeutics along with their unique mode of action has prompted widespread interest in this area. Notable developments include the discovery of non-natural ligands for the apoproteins and the observation that multiple binding modes are available for these ligands in the binding site. Mutation studies on the apoproteins have revealed much about their stability and variability, and the application of an in vitro evolution method has conferred new binding specificity for unrelated ligands. These investigations hold great promise for the application of the apoproteins for drug-delivery, transport and stabilisation systems.",
keywords = "Anti-Bacterial Agents, Ligands, Models, Molecular, Molecular Probes, Mutation, Proteins, Zinostatin",
author = "Baker, {James R.} and Woolfson, {Derek N.} and Muskett, {Frederick W.} and Stoneman, {Rhys G.} and Urbaniak, {Michael D.} and Stephen Caddick",
year = "2007",
month = may,
day = "7",
doi = "10.1002/cbic.200600534",
language = "English",
volume = "8",
pages = "704--717",
journal = "ChemBioChem",
issn = "1439-4227",
publisher = "Wiley-VCH Verlag",
number = "7",

}

RIS

TY - JOUR

T1 - Protein-small molecule interactions in neocarzinostatin, the prototypical enediyne chromoprotein antibiotic

AU - Baker, James R.

AU - Woolfson, Derek N.

AU - Muskett, Frederick W.

AU - Stoneman, Rhys G.

AU - Urbaniak, Michael D.

AU - Caddick, Stephen

PY - 2007/5/7

Y1 - 2007/5/7

N2 - The enediyne chromoproteins are a class of potent antitumour antibiotics comprising a 1:1 complex of a protein and a noncovalently bound chromophore. The protein is required to protect and transport the highly labile chromophore, which acts as the cytotoxic component by reacting with DNA leading to strand cleavage. A derivative of the best-studied member of this class, neocarzinostatin (NCS), is currently in use as a chemotherapeutic in Japan. The application of the chromoproteins as therapeutics along with their unique mode of action has prompted widespread interest in this area. Notable developments include the discovery of non-natural ligands for the apoproteins and the observation that multiple binding modes are available for these ligands in the binding site. Mutation studies on the apoproteins have revealed much about their stability and variability, and the application of an in vitro evolution method has conferred new binding specificity for unrelated ligands. These investigations hold great promise for the application of the apoproteins for drug-delivery, transport and stabilisation systems.

AB - The enediyne chromoproteins are a class of potent antitumour antibiotics comprising a 1:1 complex of a protein and a noncovalently bound chromophore. The protein is required to protect and transport the highly labile chromophore, which acts as the cytotoxic component by reacting with DNA leading to strand cleavage. A derivative of the best-studied member of this class, neocarzinostatin (NCS), is currently in use as a chemotherapeutic in Japan. The application of the chromoproteins as therapeutics along with their unique mode of action has prompted widespread interest in this area. Notable developments include the discovery of non-natural ligands for the apoproteins and the observation that multiple binding modes are available for these ligands in the binding site. Mutation studies on the apoproteins have revealed much about their stability and variability, and the application of an in vitro evolution method has conferred new binding specificity for unrelated ligands. These investigations hold great promise for the application of the apoproteins for drug-delivery, transport and stabilisation systems.

KW - Anti-Bacterial Agents

KW - Ligands

KW - Models, Molecular

KW - Molecular Probes

KW - Mutation

KW - Proteins

KW - Zinostatin

U2 - 10.1002/cbic.200600534

DO - 10.1002/cbic.200600534

M3 - Journal article

C2 - 17451164

VL - 8

SP - 704

EP - 717

JO - ChemBioChem

JF - ChemBioChem

SN - 1439-4227

IS - 7

ER -