Pulsed laser deposition of magnesium-doped calcium phosphate coatings on porous polycaprolactone scaffolds produced by rapid prototyping

School of Engineering

Text available via DOI:

https://doi.org/10.1016/j.matlet.2015.02.074
Final published version

Keywords

Biomaterials, composite materials, mineralization, osteoblasts, bone tissue engineering

View graph of relations

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Published

Standard

Pulsed laser deposition of magnesium-doped calcium phosphate coatings on porous polycaprolactone scaffolds produced by rapid prototyping. / Vandrovcova, Marta; Douglas, Timothy E.L.; Mróz, Waldemar et al.
In: Materials Letters, Vol. 148, 23.02.2015, p. 178-183.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Bibtex

@article{7e8fd2b5b3c04423848b01432bd0f079,

title = "Pulsed laser deposition of magnesium-doped calcium phosphate coatings on porous polycaprolactone scaffolds produced by rapid prototyping",

abstract = "Polycaprolactone (PCL) is a biodegradable and biocompatible polyester whose low melting point facilitates production of 3D porous scaffolds with precisely defined dimensions and internal architecture by rapid prototyping techniques. To improve the suitability of such PCL scaffolds for bone regeneration applications, they were coated with inorganic layers of calcium phosphate (CaP) and CaP doped with 0.6% w/v magnesium (CaP+Mg) using pulsed laser deposition (PLD) and characterized in vitro using osteoblast-like Saos-2 cells.Saos-2 cells were able to adhere to all scaffolds. CaP+Mg coatings significantly increased activity of alkaline phosphatase (ALP), an early differentiation marker, after 7 days. However, gene expression of ALP after 7 days was markedly lower on the same scaffolds.These data show the feasibility of coating PCL with CaP layers by PLD and the possibility of influencing osteoblastic differentiation by magnesium doping of the CaP coating.",

keywords = "Biomaterials, composite materials, mineralization, osteoblasts, bone tissue engineering",

author = "Marta Vandrovcova and Douglas, {Timothy E.L.} and Waldemar Mr{\'o}z and Olga Musial and David Schaubroeck and Boguslaw Budner and Renata Syroka and Peter Dubruel and Lucie Bacakova",

year = "2015",

month = feb,

day = "23",

doi = "10.1016/j.matlet.2015.02.074",

language = "English",

volume = "148",

pages = "178--183",

journal = "Materials Letters",

issn = "0167-577X",

publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Pulsed laser deposition of magnesium-doped calcium phosphate coatings on porous polycaprolactone scaffolds produced by rapid prototyping

AU - Vandrovcova, Marta

AU - Douglas, Timothy E.L.

AU - Mróz, Waldemar

AU - Musial, Olga

AU - Schaubroeck, David

AU - Budner, Boguslaw

AU - Syroka, Renata

AU - Dubruel, Peter

AU - Bacakova, Lucie

PY - 2015/2/23

Y1 - 2015/2/23

N2 - Polycaprolactone (PCL) is a biodegradable and biocompatible polyester whose low melting point facilitates production of 3D porous scaffolds with precisely defined dimensions and internal architecture by rapid prototyping techniques. To improve the suitability of such PCL scaffolds for bone regeneration applications, they were coated with inorganic layers of calcium phosphate (CaP) and CaP doped with 0.6% w/v magnesium (CaP+Mg) using pulsed laser deposition (PLD) and characterized in vitro using osteoblast-like Saos-2 cells.Saos-2 cells were able to adhere to all scaffolds. CaP+Mg coatings significantly increased activity of alkaline phosphatase (ALP), an early differentiation marker, after 7 days. However, gene expression of ALP after 7 days was markedly lower on the same scaffolds.These data show the feasibility of coating PCL with CaP layers by PLD and the possibility of influencing osteoblastic differentiation by magnesium doping of the CaP coating.

AB - Polycaprolactone (PCL) is a biodegradable and biocompatible polyester whose low melting point facilitates production of 3D porous scaffolds with precisely defined dimensions and internal architecture by rapid prototyping techniques. To improve the suitability of such PCL scaffolds for bone regeneration applications, they were coated with inorganic layers of calcium phosphate (CaP) and CaP doped with 0.6% w/v magnesium (CaP+Mg) using pulsed laser deposition (PLD) and characterized in vitro using osteoblast-like Saos-2 cells.Saos-2 cells were able to adhere to all scaffolds. CaP+Mg coatings significantly increased activity of alkaline phosphatase (ALP), an early differentiation marker, after 7 days. However, gene expression of ALP after 7 days was markedly lower on the same scaffolds.These data show the feasibility of coating PCL with CaP layers by PLD and the possibility of influencing osteoblastic differentiation by magnesium doping of the CaP coating.

KW - Biomaterials

KW - composite materials

KW - mineralization

KW - osteoblasts

KW - bone tissue engineering

U2 - 10.1016/j.matlet.2015.02.074

DO - 10.1016/j.matlet.2015.02.074

M3 - Journal article

VL - 148

SP - 178

EP - 183

JO - Materials Letters

JF - Materials Letters

SN - 0167-577X

ER -

Research

Links

Text available via DOI:

Keywords