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Rad18 Is Required for DNA Repair and Checkpoint Responses in Fission Yeast.

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Rad18 Is Required for DNA Repair and Checkpoint Responses in Fission Yeast. / Verkade, Heather M.; Bugg, Sarah J.; Lindsay, Howard D.; Carr, Anthony M.; O'Connell, Matthew J.

In: Molecular Biology of the Cell, Vol. 10, No. 9, 09.1999, p. 2905-2918.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Verkade, HM, Bugg, SJ, Lindsay, HD, Carr, AM & O'Connell, MJ 1999, 'Rad18 Is Required for DNA Repair and Checkpoint Responses in Fission Yeast.', Molecular Biology of the Cell, vol. 10, no. 9, pp. 2905-2918. <http://www.molbiolcell.org/cgi/content/abstract/10/9/2905>

APA

Verkade, H. M., Bugg, S. J., Lindsay, H. D., Carr, A. M., & O'Connell, M. J. (1999). Rad18 Is Required for DNA Repair and Checkpoint Responses in Fission Yeast. Molecular Biology of the Cell, 10(9), 2905-2918. http://www.molbiolcell.org/cgi/content/abstract/10/9/2905

Vancouver

Verkade HM, Bugg SJ, Lindsay HD, Carr AM, O'Connell MJ. Rad18 Is Required for DNA Repair and Checkpoint Responses in Fission Yeast. Molecular Biology of the Cell. 1999 Sep;10(9):2905-2918.

Author

Verkade, Heather M. ; Bugg, Sarah J. ; Lindsay, Howard D. ; Carr, Anthony M. ; O'Connell, Matthew J. / Rad18 Is Required for DNA Repair and Checkpoint Responses in Fission Yeast. In: Molecular Biology of the Cell. 1999 ; Vol. 10, No. 9. pp. 2905-2918.

Bibtex

@article{56fa1ee9eb2f4e2dba96aee25f7c9b55,
title = "Rad18 Is Required for DNA Repair and Checkpoint Responses in Fission Yeast.",
abstract = "To survive damage to the genome, cells must respond by activating both DNA repair and checkpoint responses. Using genetic screens in the fission yeast Schizosaccharomyces pombe, we recently isolated new genes required for DNA damage checkpoint control. We show here that one of these strains defines a new allele of the previously described rad18 gene, rad18-74. rad18 is an essential gene, even in the absence of extrinsic DNA damage. It encodes a conserved protein related to the structural maintenance of chromosomes proteins. Point mutations in rad18 lead to defective DNA repair pathways responding to both UV-induced lesions and, as we show here, double-stranded breaks. Furthermore, rad18p is required to maintain cell cycle arrest in the presence of DNA damage, and failure of this leads to highly aberrant mitoses. A gene encoding a BRCT-containing protein, brc1, was isolated as an allele-specific high-copy suppressor of rad18-74. brc1 is required for mitotic fidelity and for cellular viability in strains with rad18 mutations but is not essential for DNA damage responses. Mutations in rad18 and brc1 are synthetically lethal with a topoisomerase II mutant (top2-191), indicating that these proteins play a role in chromatin organization. These studies show a role for chromatin organization in the maintenance or activation of responses to DNA damage.",
author = "Verkade, {Heather M.} and Bugg, {Sarah J.} and Lindsay, {Howard D.} and Carr, {Anthony M.} and O'Connell, {Matthew J.}",
year = "1999",
month = sep,
language = "English",
volume = "10",
pages = "2905--2918",
journal = "Molecular Biology of the Cell",
issn = "1059-1524",
publisher = "American Society for Cell Biology",
number = "9",

}

RIS

TY - JOUR

T1 - Rad18 Is Required for DNA Repair and Checkpoint Responses in Fission Yeast.

AU - Verkade, Heather M.

AU - Bugg, Sarah J.

AU - Lindsay, Howard D.

AU - Carr, Anthony M.

AU - O'Connell, Matthew J.

PY - 1999/9

Y1 - 1999/9

N2 - To survive damage to the genome, cells must respond by activating both DNA repair and checkpoint responses. Using genetic screens in the fission yeast Schizosaccharomyces pombe, we recently isolated new genes required for DNA damage checkpoint control. We show here that one of these strains defines a new allele of the previously described rad18 gene, rad18-74. rad18 is an essential gene, even in the absence of extrinsic DNA damage. It encodes a conserved protein related to the structural maintenance of chromosomes proteins. Point mutations in rad18 lead to defective DNA repair pathways responding to both UV-induced lesions and, as we show here, double-stranded breaks. Furthermore, rad18p is required to maintain cell cycle arrest in the presence of DNA damage, and failure of this leads to highly aberrant mitoses. A gene encoding a BRCT-containing protein, brc1, was isolated as an allele-specific high-copy suppressor of rad18-74. brc1 is required for mitotic fidelity and for cellular viability in strains with rad18 mutations but is not essential for DNA damage responses. Mutations in rad18 and brc1 are synthetically lethal with a topoisomerase II mutant (top2-191), indicating that these proteins play a role in chromatin organization. These studies show a role for chromatin organization in the maintenance or activation of responses to DNA damage.

AB - To survive damage to the genome, cells must respond by activating both DNA repair and checkpoint responses. Using genetic screens in the fission yeast Schizosaccharomyces pombe, we recently isolated new genes required for DNA damage checkpoint control. We show here that one of these strains defines a new allele of the previously described rad18 gene, rad18-74. rad18 is an essential gene, even in the absence of extrinsic DNA damage. It encodes a conserved protein related to the structural maintenance of chromosomes proteins. Point mutations in rad18 lead to defective DNA repair pathways responding to both UV-induced lesions and, as we show here, double-stranded breaks. Furthermore, rad18p is required to maintain cell cycle arrest in the presence of DNA damage, and failure of this leads to highly aberrant mitoses. A gene encoding a BRCT-containing protein, brc1, was isolated as an allele-specific high-copy suppressor of rad18-74. brc1 is required for mitotic fidelity and for cellular viability in strains with rad18 mutations but is not essential for DNA damage responses. Mutations in rad18 and brc1 are synthetically lethal with a topoisomerase II mutant (top2-191), indicating that these proteins play a role in chromatin organization. These studies show a role for chromatin organization in the maintenance or activation of responses to DNA damage.

M3 - Journal article

VL - 10

SP - 2905

EP - 2918

JO - Molecular Biology of the Cell

JF - Molecular Biology of the Cell

SN - 1059-1524

IS - 9

ER -