Final published version
Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSN › Chapter
Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSN › Chapter
}
TY - CHAP
T1 - Radiometric Determination of Rubisco Activation State and Quantity in Leaves
AU - Ashton, Catherine J.
AU - Page, Rhiannon
AU - Moreira Lobo, Ana
AU - Amaral, Joana
AU - Siqueira, Joao
AU - Orr, Douglas
AU - Carmo-Silva, Elizabete
PY - 2024/12/31
Y1 - 2024/12/31
N2 - Rubisco is the key enzyme in photosynthesis, catalyzing fixation of carbon dioxide from the atmosphere into energy storage molecules. Several inefficiencies in Rubisco limit the rate of photosynthesis, and, therefore, the growth of the plant. Rubisco is sensitive to light, making deactivation of the enzyme upon sampling likely. Moreover, the indirect methods often used to study its activity make obtaining reliable data difficult. In this Chapter, we describe an approach to generate reliable and repeatable data for Rubisco activities, activation state and abundance in plant leaves. We include methods to sample and extract proteins, minimizing Rubisco degradation and deactivation. We describe radiometric techniques to measure Rubisco activities and calculate its activation state at the time of sampling, and to quantify its abundance.
AB - Rubisco is the key enzyme in photosynthesis, catalyzing fixation of carbon dioxide from the atmosphere into energy storage molecules. Several inefficiencies in Rubisco limit the rate of photosynthesis, and, therefore, the growth of the plant. Rubisco is sensitive to light, making deactivation of the enzyme upon sampling likely. Moreover, the indirect methods often used to study its activity make obtaining reliable data difficult. In this Chapter, we describe an approach to generate reliable and repeatable data for Rubisco activities, activation state and abundance in plant leaves. We include methods to sample and extract proteins, minimizing Rubisco degradation and deactivation. We describe radiometric techniques to measure Rubisco activities and calculate its activation state at the time of sampling, and to quantify its abundance.
U2 - 10.1016/bs.mie.2024.10.018
DO - 10.1016/bs.mie.2024.10.018
M3 - Chapter
SN - 9780443313103
VL - 708
T3 - Methods in Enzymology
SP - 323
EP - 351
BT - Carboxylases
PB - Elsevier
ER -