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Raman spectroscopy for intracellular monitoring of carotenoid in blakeslea trispora

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Raman spectroscopy for intracellular monitoring of carotenoid in blakeslea trispora. / Papaioannou, Emmanouil H.; Liakopoulou-Kyriakides, Maria; Christofilos, Dimitrios et al.
In: Applied Biochemistry and Biotechnology, Vol. 159, No. 2, 11.2009, p. 478-487.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Papaioannou, EH, Liakopoulou-Kyriakides, M, Christofilos, D, Arvanitidis, I & Kourouklis, G 2009, 'Raman spectroscopy for intracellular monitoring of carotenoid in blakeslea trispora', Applied Biochemistry and Biotechnology, vol. 159, no. 2, pp. 478-487. https://doi.org/10.1007/s12010-008-8472-0

APA

Papaioannou, E. H., Liakopoulou-Kyriakides, M., Christofilos, D., Arvanitidis, I., & Kourouklis, G. (2009). Raman spectroscopy for intracellular monitoring of carotenoid in blakeslea trispora. Applied Biochemistry and Biotechnology, 159(2), 478-487. https://doi.org/10.1007/s12010-008-8472-0

Vancouver

Papaioannou EH, Liakopoulou-Kyriakides M, Christofilos D, Arvanitidis I, Kourouklis G. Raman spectroscopy for intracellular monitoring of carotenoid in blakeslea trispora. Applied Biochemistry and Biotechnology. 2009 Nov;159(2):478-487. Epub 2009 Jan 6. doi: 10.1007/s12010-008-8472-0

Author

Papaioannou, Emmanouil H. ; Liakopoulou-Kyriakides, Maria ; Christofilos, Dimitrios et al. / Raman spectroscopy for intracellular monitoring of carotenoid in blakeslea trispora. In: Applied Biochemistry and Biotechnology. 2009 ; Vol. 159, No. 2. pp. 478-487.

Bibtex

@article{6c02c5d28df34634a0ae17b831a2012e,
title = "Raman spectroscopy for intracellular monitoring of carotenoid in blakeslea trispora",
abstract = "In the present study, we explore the feasibility of Raman spectroscopy for intracellular monitoring of carotenoid in filamentous fungi Blakeslea trispora. Although carotenoid production from this fungus has been extensively studied through various chromatographic methods and ultraviolet-visible spectroscopy, no intracellular monitoring has been demonstrated until now. The intensity of the Raman spectrum, and more conveniently that of the strongest ν 1 carotenoid band at ∼1,519 cm-1, exhibits a good linear correlation with the carotenoid content of the sample as determined by high-performance liquid chromatography (HPLC) and ultraviolet-visible (UV-Vis) spectroscopy. Our results suggest that Raman spectroscopy can serve as an alternative method for the study and quantification of carotenoid in batch-mated submerged cultivations of B. trispora and similar organisms. Although not as accurate as HPLC, it allows a rapid sampling and analysis, avoiding the prolonged and tedious classical isolation procedures required for carotenoid determination by HPLC and UV-Vis spectroscopy.",
keywords = "β-carotene, Blakeslea trispora, Carotenoid, Lycopene, Raman spectroscopy, RP-HPLC-DAD",
author = "Papaioannou, {Emmanouil H.} and Maria Liakopoulou-Kyriakides and Dimitrios Christofilos and Ioannis Arvanitidis and Gerasimos Kourouklis",
year = "2009",
month = nov,
doi = "10.1007/s12010-008-8472-0",
language = "English",
volume = "159",
pages = "478--487",
journal = "Applied Biochemistry and Biotechnology",
issn = "0273-2289",
publisher = "Humana Press",
number = "2",

}

RIS

TY - JOUR

T1 - Raman spectroscopy for intracellular monitoring of carotenoid in blakeslea trispora

AU - Papaioannou, Emmanouil H.

AU - Liakopoulou-Kyriakides, Maria

AU - Christofilos, Dimitrios

AU - Arvanitidis, Ioannis

AU - Kourouklis, Gerasimos

PY - 2009/11

Y1 - 2009/11

N2 - In the present study, we explore the feasibility of Raman spectroscopy for intracellular monitoring of carotenoid in filamentous fungi Blakeslea trispora. Although carotenoid production from this fungus has been extensively studied through various chromatographic methods and ultraviolet-visible spectroscopy, no intracellular monitoring has been demonstrated until now. The intensity of the Raman spectrum, and more conveniently that of the strongest ν 1 carotenoid band at ∼1,519 cm-1, exhibits a good linear correlation with the carotenoid content of the sample as determined by high-performance liquid chromatography (HPLC) and ultraviolet-visible (UV-Vis) spectroscopy. Our results suggest that Raman spectroscopy can serve as an alternative method for the study and quantification of carotenoid in batch-mated submerged cultivations of B. trispora and similar organisms. Although not as accurate as HPLC, it allows a rapid sampling and analysis, avoiding the prolonged and tedious classical isolation procedures required for carotenoid determination by HPLC and UV-Vis spectroscopy.

AB - In the present study, we explore the feasibility of Raman spectroscopy for intracellular monitoring of carotenoid in filamentous fungi Blakeslea trispora. Although carotenoid production from this fungus has been extensively studied through various chromatographic methods and ultraviolet-visible spectroscopy, no intracellular monitoring has been demonstrated until now. The intensity of the Raman spectrum, and more conveniently that of the strongest ν 1 carotenoid band at ∼1,519 cm-1, exhibits a good linear correlation with the carotenoid content of the sample as determined by high-performance liquid chromatography (HPLC) and ultraviolet-visible (UV-Vis) spectroscopy. Our results suggest that Raman spectroscopy can serve as an alternative method for the study and quantification of carotenoid in batch-mated submerged cultivations of B. trispora and similar organisms. Although not as accurate as HPLC, it allows a rapid sampling and analysis, avoiding the prolonged and tedious classical isolation procedures required for carotenoid determination by HPLC and UV-Vis spectroscopy.

KW - β-carotene

KW - Blakeslea trispora

KW - Carotenoid

KW - Lycopene

KW - Raman spectroscopy

KW - RP-HPLC-DAD

U2 - 10.1007/s12010-008-8472-0

DO - 10.1007/s12010-008-8472-0

M3 - Journal article

C2 - 19130307

AN - SCOPUS:70350662499

VL - 159

SP - 478

EP - 487

JO - Applied Biochemistry and Biotechnology

JF - Applied Biochemistry and Biotechnology

SN - 0273-2289

IS - 2

ER -