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Recovery of a bacterial sub-population from sewage using immunofluorescent flow cytometry and cell sorting

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Recovery of a bacterial sub-population from sewage using immunofluorescent flow cytometry and cell sorting. / Porter, Jonathan; Robinson, Joan; Pickup, Roger et al.
In: FEMS Microbiology Letters, Vol. 133, No. 1-2, 01.11.1995, p. 195-199.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Porter, J, Robinson, J, Pickup, R & Edwards, C 1995, 'Recovery of a bacterial sub-population from sewage using immunofluorescent flow cytometry and cell sorting', FEMS Microbiology Letters, vol. 133, no. 1-2, pp. 195-199. https://doi.org/10.1016/0378-1097(95)00371-B

APA

Porter, J., Robinson, J., Pickup, R., & Edwards, C. (1995). Recovery of a bacterial sub-population from sewage using immunofluorescent flow cytometry and cell sorting. FEMS Microbiology Letters, 133(1-2), 195-199. https://doi.org/10.1016/0378-1097(95)00371-B

Vancouver

Porter J, Robinson J, Pickup R, Edwards C. Recovery of a bacterial sub-population from sewage using immunofluorescent flow cytometry and cell sorting. FEMS Microbiology Letters. 1995 Nov 1;133(1-2):195-199. doi: 10.1016/0378-1097(95)00371-B

Author

Porter, Jonathan ; Robinson, Joan ; Pickup, Roger et al. / Recovery of a bacterial sub-population from sewage using immunofluorescent flow cytometry and cell sorting. In: FEMS Microbiology Letters. 1995 ; Vol. 133, No. 1-2. pp. 195-199.

Bibtex

@article{0d1b028990504668a65f812724dd4945,
title = "Recovery of a bacterial sub-population from sewage using immunofluorescent flow cytometry and cell sorting",
abstract = "The effectiveness of immunofluorescence flow cytometry and cell sorting to detect, quantify and separate indigenous bacterial populations present in low concentrations in sewage outflow was investigated. Preparatory experiments for targeted recovery revealed indigenous, immunoglobulin-G-binding particles present at low levels in sewage outflow samples taken from Coniston Water. Fluorescence-activated cell sorting of this population was employed to enrich for these particles, which were confirmed as bacterial cells. This cell population comprised approximately 23% of the total plate count on MacConkey agar before cell sorting, rising to approximately 95% after sorting. These results corresponded to cell densities of less than 5% of the total plate count on R2A agar. Taxonomic tests suggested the bacterium to be Ochrobactrum anthropi.",
keywords = "Cell sorting, Flow cytometry, Ochrobactrum anthropi",
author = "Jonathan Porter and Joan Robinson and Roger Pickup and Clive Edwards",
year = "1995",
month = nov,
day = "1",
doi = "10.1016/0378-1097(95)00371-B",
language = "English",
volume = "133",
pages = "195--199",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Wiley-Blackwell",
number = "1-2",

}

RIS

TY - JOUR

T1 - Recovery of a bacterial sub-population from sewage using immunofluorescent flow cytometry and cell sorting

AU - Porter, Jonathan

AU - Robinson, Joan

AU - Pickup, Roger

AU - Edwards, Clive

PY - 1995/11/1

Y1 - 1995/11/1

N2 - The effectiveness of immunofluorescence flow cytometry and cell sorting to detect, quantify and separate indigenous bacterial populations present in low concentrations in sewage outflow was investigated. Preparatory experiments for targeted recovery revealed indigenous, immunoglobulin-G-binding particles present at low levels in sewage outflow samples taken from Coniston Water. Fluorescence-activated cell sorting of this population was employed to enrich for these particles, which were confirmed as bacterial cells. This cell population comprised approximately 23% of the total plate count on MacConkey agar before cell sorting, rising to approximately 95% after sorting. These results corresponded to cell densities of less than 5% of the total plate count on R2A agar. Taxonomic tests suggested the bacterium to be Ochrobactrum anthropi.

AB - The effectiveness of immunofluorescence flow cytometry and cell sorting to detect, quantify and separate indigenous bacterial populations present in low concentrations in sewage outflow was investigated. Preparatory experiments for targeted recovery revealed indigenous, immunoglobulin-G-binding particles present at low levels in sewage outflow samples taken from Coniston Water. Fluorescence-activated cell sorting of this population was employed to enrich for these particles, which were confirmed as bacterial cells. This cell population comprised approximately 23% of the total plate count on MacConkey agar before cell sorting, rising to approximately 95% after sorting. These results corresponded to cell densities of less than 5% of the total plate count on R2A agar. Taxonomic tests suggested the bacterium to be Ochrobactrum anthropi.

KW - Cell sorting

KW - Flow cytometry

KW - Ochrobactrum anthropi

U2 - 10.1016/0378-1097(95)00371-B

DO - 10.1016/0378-1097(95)00371-B

M3 - Journal article

AN - SCOPUS:0028826315

VL - 133

SP - 195

EP - 199

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 1-2

ER -