Redirection of T cell-mediated cytotoxicity by a recombinant single-chain Fv molecule.

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http://www.jimmunol.org/cgi/content/abstract/152/4/1802

Research output: Contribution to Journal/Magazine › Journal article

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Redirection of T cell-mediated cytotoxicity by a recombinant single-chain Fv molecule. / George, A. J. T.; Titus, J. A.; Jost, C. R. et al.
In: Journal of Immunology, Vol. 152, No. 4, 02.1994, p. 1802-1811.

Research output: Contribution to Journal/Magazine › Journal article

Harvard

George, AJT, Titus, JA, Jost, CR, Kurucz, I, Perez, P, Andrew, SM, Nicholls, PJ, Huston, JS & Segal, DM 1994, 'Redirection of T cell-mediated cytotoxicity by a recombinant single-chain Fv molecule.', Journal of Immunology, vol. 152, no. 4, pp. 1802-1811. <http://www.jimmunol.org/cgi/content/abstract/152/4/1802>

APA

George, A. J. T., Titus, J. A., Jost, C. R., Kurucz, I., Perez, P., Andrew, S. M., Nicholls, P. J., Huston, J. S., & Segal, D. M. (1994). Redirection of T cell-mediated cytotoxicity by a recombinant single-chain Fv molecule. Journal of Immunology, 152(4), 1802-1811. http://www.jimmunol.org/cgi/content/abstract/152/4/1802

Vancouver

George AJT, Titus JA, Jost CR, Kurucz I, Perez P, Andrew SM et al. Redirection of T cell-mediated cytotoxicity by a recombinant single-chain Fv molecule. Journal of Immunology. 1994 Feb;152(4):1802-1811.

Author

George, A. J. T. ; Titus, J. A. ; Jost, C. R. et al. / Redirection of T cell-mediated cytotoxicity by a recombinant single-chain Fv molecule. In: Journal of Immunology. 1994 ; Vol. 152, No. 4. pp. 1802-1811.

Bibtex

@article{9695f7b6eb2742d3a733b43e64cb922d,

title = "Redirection of T cell-mediated cytotoxicity by a recombinant single-chain Fv molecule.",

abstract = "We have produced two single-chain Fv (sFv) proteins by bacterial periplasmic secretion, one sFv with specificity for the hapten DNP, and the other for the human transferrin receptor. After solubilization and refolding, we recovered several mg of active sFv per liter of bacterial culture. Each sFv bound to cells bearing the appropriate Ag and could be used to direct targeted cellular cytotoxicity. Targeting relied on a universal bispecific antibody designed to cross-link CD3 on the cytotoxic T cell with a peptide fused to the sFv carboxyl-terminus. The universal bispecific antibody was used in combination with the Ag- specific sFv to redirect human cytotoxic T cells to kill a variety of target cells. Such an approach has a number of advantages that may make it useful for the immunotherapy of cancer and other diseases.",

author = "George, {A. J. T.} and Titus, {J. A.} and Jost, {C. R.} and I. Kurucz and P. Perez and Andrew, {S. M.} and Nicholls, {P. J.} and Huston, {J. S.} and Segal, {D. M.}",

year = "1994",

month = feb,

language = "English",

volume = "152",

pages = "1802--1811",

journal = "Journal of Immunology",

issn = "0022-1767",

publisher = "American Association of Immunologists",

number = "4",

}

RIS

TY - JOUR

T1 - Redirection of T cell-mediated cytotoxicity by a recombinant single-chain Fv molecule.

AU - George, A. J. T.

AU - Titus, J. A.

AU - Jost, C. R.

AU - Kurucz, I.

AU - Perez, P.

AU - Andrew, S. M.

AU - Nicholls, P. J.

AU - Huston, J. S.

AU - Segal, D. M.

PY - 1994/2

Y1 - 1994/2

N2 - We have produced two single-chain Fv (sFv) proteins by bacterial periplasmic secretion, one sFv with specificity for the hapten DNP, and the other for the human transferrin receptor. After solubilization and refolding, we recovered several mg of active sFv per liter of bacterial culture. Each sFv bound to cells bearing the appropriate Ag and could be used to direct targeted cellular cytotoxicity. Targeting relied on a universal bispecific antibody designed to cross-link CD3 on the cytotoxic T cell with a peptide fused to the sFv carboxyl-terminus. The universal bispecific antibody was used in combination with the Ag- specific sFv to redirect human cytotoxic T cells to kill a variety of target cells. Such an approach has a number of advantages that may make it useful for the immunotherapy of cancer and other diseases.

AB - We have produced two single-chain Fv (sFv) proteins by bacterial periplasmic secretion, one sFv with specificity for the hapten DNP, and the other for the human transferrin receptor. After solubilization and refolding, we recovered several mg of active sFv per liter of bacterial culture. Each sFv bound to cells bearing the appropriate Ag and could be used to direct targeted cellular cytotoxicity. Targeting relied on a universal bispecific antibody designed to cross-link CD3 on the cytotoxic T cell with a peptide fused to the sFv carboxyl-terminus. The universal bispecific antibody was used in combination with the Ag- specific sFv to redirect human cytotoxic T cells to kill a variety of target cells. Such an approach has a number of advantages that may make it useful for the immunotherapy of cancer and other diseases.

M3 - Journal article

VL - 152

SP - 1802

EP - 1811

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 4

ER -

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