Final published version
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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Redistribution of FLAgellar Member 8 during the trypanosome life cycle
T2 - Consequences for cell fate prediction
AU - Calvo-Álvarez, Estefanía
AU - Bonnefoy, Serge
AU - Salles, Audrey
AU - Benson, Fiona E
AU - McKean, Paul G
AU - Bastin, Philippe
AU - Rotureau, Brice
N1 - © 2021 Instuit Pasteur. Cellular Microbiology published by John Wiley & Sons Ltd.
PY - 2021/9/30
Y1 - 2021/9/30
N2 - The single flagellum of African trypanosomes is essential in multiple aspects of the parasites' development. The FLAgellar Member 8 protein (FLAM8), localised to the tip of the flagellum in cultured insect forms of Trypanosoma brucei, was identified as a marker of the locking event that controls flagellum length. Here, we investigated whether FLAM8 could also reflect the flagellum maturation state in other parasite cycle stages. We observed that FLAM8 distribution extended along the entire flagellar cytoskeleton in mammalian-infective forms. Then, a rapid FLAM8 concentration to the distal tip occurs during differentiation into early insect forms, illustrating the remodelling of an existing flagellum. In the tsetse cardia, FLAM8 further localises to the entire length of the new flagellum during an asymmetric division. Strikingly, in parasites dividing in the tsetse midgut and in the salivary glands, the amount and distribution of FLAM8 in the new flagellum were seen to predict the daughter cell fate. We propose and discuss how FLAM8 could be considered a meta-marker of the flagellum stage and maturation state in trypanosomes.
AB - The single flagellum of African trypanosomes is essential in multiple aspects of the parasites' development. The FLAgellar Member 8 protein (FLAM8), localised to the tip of the flagellum in cultured insect forms of Trypanosoma brucei, was identified as a marker of the locking event that controls flagellum length. Here, we investigated whether FLAM8 could also reflect the flagellum maturation state in other parasite cycle stages. We observed that FLAM8 distribution extended along the entire flagellar cytoskeleton in mammalian-infective forms. Then, a rapid FLAM8 concentration to the distal tip occurs during differentiation into early insect forms, illustrating the remodelling of an existing flagellum. In the tsetse cardia, FLAM8 further localises to the entire length of the new flagellum during an asymmetric division. Strikingly, in parasites dividing in the tsetse midgut and in the salivary glands, the amount and distribution of FLAM8 in the new flagellum were seen to predict the daughter cell fate. We propose and discuss how FLAM8 could be considered a meta-marker of the flagellum stage and maturation state in trypanosomes.
KW - differentiation
KW - division
KW - elongation
KW - flagellum
KW - FLAM8
KW - remodelling
KW - Trypanosoma brucei
U2 - 10.1111/cmi.13347
DO - 10.1111/cmi.13347
M3 - Journal article
C2 - 33896083
VL - 23
JO - Cellular Microbiology
JF - Cellular Microbiology
SN - 1462-5814
IS - 9
M1 - e13347
ER -