Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Reduced CD4 T Lymphocytes in Lymph Nodes of the Mouse Model of Autism Induced by Valproic Acid
AU - Baronio, Diego
AU - Bauer-Negrini, Guilherme
AU - Castro, Kamila
AU - Della-Flora Nunes, Gustavo
AU - Riesgo, Rudimar
AU - Mendes-da-Cruz, Daniella Arêas
AU - Savino, Wilson
AU - Gottfried, Carmem
AU - Bambini-Junior, Victorio
N1 - © 2018 S. Karger AG, Basel.
PY - 2019/2/28
Y1 - 2019/2/28
N2 - OBJECTIVE: Considering the potential role of lymphocytes in the pathophysiology of autism spectrum disorder (ASD), we aimed to evaluate possible alterations of T cell pools in the lymphoid organs of an animal model of autism induced by valproic acid (VPA). Pregnant Swiss mice received a single intraperitoneal injection of 600 mg/kg of VPA (VPA group) or saline (control group) on day 11 of gestation. Male offspring were euthanized on postnatal day 60 for removal of thy-muses, spleens, and a pool of inguinal, axillary and brachial lymph nodes. Cellularity was evaluated, and flow cytometry analysis was performed on cell suspensions incubated with the mouse antibodies anti-CD3-FITC, anti-CD4-PE, and anti-CD8-PE-Cy7. We observed that the prenatal exposure to VPA induced a reduction in the numbers of CD3+CD4+ T cells in their lymph nodes when compared to the control animals. This was specific since it was not seen in the thymus or spleen. The consistent decrease in the number of CD4+ T cells in subcutaneous lymph nodes of mice from the animal model of autism may be related to the allergic symptoms frequently observed in ASD. Further research is necessary to characterize the immunological patterns in ASD and the connection with the pathophysiology of this disorder.
AB - OBJECTIVE: Considering the potential role of lymphocytes in the pathophysiology of autism spectrum disorder (ASD), we aimed to evaluate possible alterations of T cell pools in the lymphoid organs of an animal model of autism induced by valproic acid (VPA). Pregnant Swiss mice received a single intraperitoneal injection of 600 mg/kg of VPA (VPA group) or saline (control group) on day 11 of gestation. Male offspring were euthanized on postnatal day 60 for removal of thy-muses, spleens, and a pool of inguinal, axillary and brachial lymph nodes. Cellularity was evaluated, and flow cytometry analysis was performed on cell suspensions incubated with the mouse antibodies anti-CD3-FITC, anti-CD4-PE, and anti-CD8-PE-Cy7. We observed that the prenatal exposure to VPA induced a reduction in the numbers of CD3+CD4+ T cells in their lymph nodes when compared to the control animals. This was specific since it was not seen in the thymus or spleen. The consistent decrease in the number of CD4+ T cells in subcutaneous lymph nodes of mice from the animal model of autism may be related to the allergic symptoms frequently observed in ASD. Further research is necessary to characterize the immunological patterns in ASD and the connection with the pathophysiology of this disorder.
KW - Animals
KW - Autistic Disorder/chemically induced
KW - CD4-Positive T-Lymphocytes/immunology
KW - Disease Models, Animal
KW - Enzyme Inhibitors/toxicity
KW - Female
KW - Lymph Nodes/immunology
KW - Male
KW - Mice
KW - Pregnancy
KW - Prenatal Exposure Delayed Effects/chemically induced
KW - Valproic Acid/toxicity
U2 - 10.1159/000491395
DO - 10.1159/000491395
M3 - Journal article
C2 - 30121669
VL - 25
SP - 280
EP - 284
JO - Neuroimmunomodulation
JF - Neuroimmunomodulation
SN - 1021-7401
IS - 5-6
ER -