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Regulation of GmNRT2 expression and nitrate transport activity in roots of soybean (Glycine max)

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Regulation of GmNRT2 expression and nitrate transport activity in roots of soybean (Glycine max). / Ranamalie Amarasinghe, B. H.R.; De Bruxelles, Guy L.; Braddon, Marysha et al.
In: Planta, Vol. 206, No. 1, 30.07.1998, p. 44-52.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Ranamalie Amarasinghe, BHR, De Bruxelles, GL, Braddon, M, Onyeocha, I, Forde, BG & Udvardi, MK 1998, 'Regulation of GmNRT2 expression and nitrate transport activity in roots of soybean (Glycine max)', Planta, vol. 206, no. 1, pp. 44-52. https://doi.org/10.1007/s004250050372

APA

Ranamalie Amarasinghe, B. H. R., De Bruxelles, G. L., Braddon, M., Onyeocha, I., Forde, B. G., & Udvardi, M. K. (1998). Regulation of GmNRT2 expression and nitrate transport activity in roots of soybean (Glycine max). Planta, 206(1), 44-52. https://doi.org/10.1007/s004250050372

Vancouver

Ranamalie Amarasinghe BHR, De Bruxelles GL, Braddon M, Onyeocha I, Forde BG, Udvardi MK. Regulation of GmNRT2 expression and nitrate transport activity in roots of soybean (Glycine max). Planta. 1998 Jul 30;206(1):44-52. doi: 10.1007/s004250050372

Author

Ranamalie Amarasinghe, B. H.R. ; De Bruxelles, Guy L. ; Braddon, Marysha et al. / Regulation of GmNRT2 expression and nitrate transport activity in roots of soybean (Glycine max). In: Planta. 1998 ; Vol. 206, No. 1. pp. 44-52.

Bibtex

@article{e5452e96185240cb9473ece739a6ca33,
title = "Regulation of GmNRT2 expression and nitrate transport activity in roots of soybean (Glycine max)",
abstract = "A full-length cDNA, GmNRT2, encoding a putative high-affinity nitrate transporter was isolated from a Glycine max (L.) root cDNA library and sequenced. The deduced GmNRT2 protein is 530 amino acids in length and contains 12 putative membrane-spanning domains and a long, hydrophilic C-terminal domain. GmNRT2 is related to high-affinity nitrate transporters in the eukaryotes Chlamydomonas reinhardtii and Aspergillus nidulans, and to putative high-affinity nitrate transporters in barley and tobacco. Southern blot analysis indicated that GmNRT2 is part of a small, multigene family in soybean. Expression of GmNRT2 in roots was regulated by the type of nitrogen source provided to plants: GmNRT2 mRNA levels were barely detectable in ammonium-grown plants, higher in nitrogen-deprived plants, and highest in nitrate-grown plants. Induction of GmNRT2 mRNA levels in roots occurred within 1 h after exposure of plants to nitrate. Nitrate induction of GmNRT2 mRNA levels was accompanied by a fourfold increase in net nitrate uptake by soybean roots at 100 μM external nitrate. The molecular and physiological evidence indicates that GmNRT2 is probably a high-affinity nitrate transporter involved in nitrate uptake by soybean roots.",
keywords = "Glycine (nitrate transport), High-affinity nitrate transporter, Major facilitator superfamily, Nitrate induction, Nutrient uptake",
author = "{Ranamalie Amarasinghe}, {B. H.R.} and {De Bruxelles}, {Guy L.} and Marysha Braddon and Ignatius Onyeocha and Forde, {Brian G.} and Udvardi, {Michael K.}",
year = "1998",
month = jul,
day = "30",
doi = "10.1007/s004250050372",
language = "English",
volume = "206",
pages = "44--52",
journal = "Planta",
issn = "0032-0935",
publisher = "Springer",
number = "1",

}

RIS

TY - JOUR

T1 - Regulation of GmNRT2 expression and nitrate transport activity in roots of soybean (Glycine max)

AU - Ranamalie Amarasinghe, B. H.R.

AU - De Bruxelles, Guy L.

AU - Braddon, Marysha

AU - Onyeocha, Ignatius

AU - Forde, Brian G.

AU - Udvardi, Michael K.

PY - 1998/7/30

Y1 - 1998/7/30

N2 - A full-length cDNA, GmNRT2, encoding a putative high-affinity nitrate transporter was isolated from a Glycine max (L.) root cDNA library and sequenced. The deduced GmNRT2 protein is 530 amino acids in length and contains 12 putative membrane-spanning domains and a long, hydrophilic C-terminal domain. GmNRT2 is related to high-affinity nitrate transporters in the eukaryotes Chlamydomonas reinhardtii and Aspergillus nidulans, and to putative high-affinity nitrate transporters in barley and tobacco. Southern blot analysis indicated that GmNRT2 is part of a small, multigene family in soybean. Expression of GmNRT2 in roots was regulated by the type of nitrogen source provided to plants: GmNRT2 mRNA levels were barely detectable in ammonium-grown plants, higher in nitrogen-deprived plants, and highest in nitrate-grown plants. Induction of GmNRT2 mRNA levels in roots occurred within 1 h after exposure of plants to nitrate. Nitrate induction of GmNRT2 mRNA levels was accompanied by a fourfold increase in net nitrate uptake by soybean roots at 100 μM external nitrate. The molecular and physiological evidence indicates that GmNRT2 is probably a high-affinity nitrate transporter involved in nitrate uptake by soybean roots.

AB - A full-length cDNA, GmNRT2, encoding a putative high-affinity nitrate transporter was isolated from a Glycine max (L.) root cDNA library and sequenced. The deduced GmNRT2 protein is 530 amino acids in length and contains 12 putative membrane-spanning domains and a long, hydrophilic C-terminal domain. GmNRT2 is related to high-affinity nitrate transporters in the eukaryotes Chlamydomonas reinhardtii and Aspergillus nidulans, and to putative high-affinity nitrate transporters in barley and tobacco. Southern blot analysis indicated that GmNRT2 is part of a small, multigene family in soybean. Expression of GmNRT2 in roots was regulated by the type of nitrogen source provided to plants: GmNRT2 mRNA levels were barely detectable in ammonium-grown plants, higher in nitrogen-deprived plants, and highest in nitrate-grown plants. Induction of GmNRT2 mRNA levels in roots occurred within 1 h after exposure of plants to nitrate. Nitrate induction of GmNRT2 mRNA levels was accompanied by a fourfold increase in net nitrate uptake by soybean roots at 100 μM external nitrate. The molecular and physiological evidence indicates that GmNRT2 is probably a high-affinity nitrate transporter involved in nitrate uptake by soybean roots.

KW - Glycine (nitrate transport)

KW - High-affinity nitrate transporter

KW - Major facilitator superfamily

KW - Nitrate induction

KW - Nutrient uptake

U2 - 10.1007/s004250050372

DO - 10.1007/s004250050372

M3 - Journal article

C2 - 9715532

AN - SCOPUS:3543001606

VL - 206

SP - 44

EP - 52

JO - Planta

JF - Planta

SN - 0032-0935

IS - 1

ER -