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Regulation of Rubisco by inhibitors in the light

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Regulation of Rubisco by inhibitors in the light. / Parry, M. A J; Andralojc, P. J.; Parmar, S. et al.
In: Plant, Cell and Environment, Vol. 20, No. 4, 01.04.1997, p. 528-534.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Parry, MAJ, Andralojc, PJ, Parmar, S, Keys, AJ, Habash, D, Paul, MJ, Alred, R, Quick, WP & Servaites, JC 1997, 'Regulation of Rubisco by inhibitors in the light', Plant, Cell and Environment, vol. 20, no. 4, pp. 528-534. https://doi.org/10.1046/j.1365-3040.1997.d01-85.x

APA

Parry, M. A. J., Andralojc, P. J., Parmar, S., Keys, A. J., Habash, D., Paul, M. J., Alred, R., Quick, W. P., & Servaites, J. C. (1997). Regulation of Rubisco by inhibitors in the light. Plant, Cell and Environment, 20(4), 528-534. https://doi.org/10.1046/j.1365-3040.1997.d01-85.x

Vancouver

Parry MAJ, Andralojc PJ, Parmar S, Keys AJ, Habash D, Paul MJ et al. Regulation of Rubisco by inhibitors in the light. Plant, Cell and Environment. 1997 Apr 1;20(4):528-534. doi: 10.1046/j.1365-3040.1997.d01-85.x

Author

Parry, M. A J ; Andralojc, P. J. ; Parmar, S. et al. / Regulation of Rubisco by inhibitors in the light. In: Plant, Cell and Environment. 1997 ; Vol. 20, No. 4. pp. 528-534.

Bibtex

@article{eaff0fc3989045f39852df5b2a752569,
title = "Regulation of Rubisco by inhibitors in the light",
abstract = "2-carboxy-D-arabinitol-1-phosphate (CA1P) bound to Rubisco either in leaf extracts or after purification can be displaced by SO42- ions. Thus, treatment of leaf extracts with a buffer containing 200 mol m-3 SO42- displaces any bound CA1P and enables measurement of maximum carboxylation potential. In tobacco leaves, the activity following treatment with SO42- ions ('maximal activity') is greater than the total Rubisco activity. The ratio of the two activities altered in a dynamic way with fluctuations in irradiance. Even in species which do not produce significant amounts of CA1P, the maximal activity greatly exceeded the total activity. Anion exchange separation of components in acid extracts confirmed the absence of CA1P in tobacco leaves harvested above an irradiance of 300 μmol quanta m-2 s-1, but the presence of another inhibitor of Rubisco. These results are consistent with the regulation of Rubisco activity by inhibitors other than CA1P which, like CA1P, can be displaced by SO42-ions.",
keywords = "2 carboxy-D-arabinitol-1-phosphate, maximal activity, regulation, ribulose-1,5-bisphosphate, ribulose-1,5-bisphosphate carboxylase/oxygenase, total activity",
author = "Parry, {M. A J} and Andralojc, {P. J.} and S. Parmar and Keys, {A. J.} and D. Habash and Paul, {M. J.} and R. Alred and Quick, {W. P.} and Servaites, {J. C.}",
year = "1997",
month = apr,
day = "1",
doi = "10.1046/j.1365-3040.1997.d01-85.x",
language = "English",
volume = "20",
pages = "528--534",
journal = "Plant, Cell and Environment",
issn = "0140-7791",
publisher = "Wiley",
number = "4",

}

RIS

TY - JOUR

T1 - Regulation of Rubisco by inhibitors in the light

AU - Parry, M. A J

AU - Andralojc, P. J.

AU - Parmar, S.

AU - Keys, A. J.

AU - Habash, D.

AU - Paul, M. J.

AU - Alred, R.

AU - Quick, W. P.

AU - Servaites, J. C.

PY - 1997/4/1

Y1 - 1997/4/1

N2 - 2-carboxy-D-arabinitol-1-phosphate (CA1P) bound to Rubisco either in leaf extracts or after purification can be displaced by SO42- ions. Thus, treatment of leaf extracts with a buffer containing 200 mol m-3 SO42- displaces any bound CA1P and enables measurement of maximum carboxylation potential. In tobacco leaves, the activity following treatment with SO42- ions ('maximal activity') is greater than the total Rubisco activity. The ratio of the two activities altered in a dynamic way with fluctuations in irradiance. Even in species which do not produce significant amounts of CA1P, the maximal activity greatly exceeded the total activity. Anion exchange separation of components in acid extracts confirmed the absence of CA1P in tobacco leaves harvested above an irradiance of 300 μmol quanta m-2 s-1, but the presence of another inhibitor of Rubisco. These results are consistent with the regulation of Rubisco activity by inhibitors other than CA1P which, like CA1P, can be displaced by SO42-ions.

AB - 2-carboxy-D-arabinitol-1-phosphate (CA1P) bound to Rubisco either in leaf extracts or after purification can be displaced by SO42- ions. Thus, treatment of leaf extracts with a buffer containing 200 mol m-3 SO42- displaces any bound CA1P and enables measurement of maximum carboxylation potential. In tobacco leaves, the activity following treatment with SO42- ions ('maximal activity') is greater than the total Rubisco activity. The ratio of the two activities altered in a dynamic way with fluctuations in irradiance. Even in species which do not produce significant amounts of CA1P, the maximal activity greatly exceeded the total activity. Anion exchange separation of components in acid extracts confirmed the absence of CA1P in tobacco leaves harvested above an irradiance of 300 μmol quanta m-2 s-1, but the presence of another inhibitor of Rubisco. These results are consistent with the regulation of Rubisco activity by inhibitors other than CA1P which, like CA1P, can be displaced by SO42-ions.

KW - 2 carboxy-D-arabinitol-1-phosphate

KW - maximal activity

KW - regulation

KW - ribulose-1,5-bisphosphate

KW - ribulose-1,5-bisphosphate carboxylase/oxygenase

KW - total activity

UR - http://www.scopus.com/inward/record.url?scp=0030960912&partnerID=8YFLogxK

U2 - 10.1046/j.1365-3040.1997.d01-85.x

DO - 10.1046/j.1365-3040.1997.d01-85.x

M3 - Journal article

AN - SCOPUS:0030960912

VL - 20

SP - 528

EP - 534

JO - Plant, Cell and Environment

JF - Plant, Cell and Environment

SN - 0140-7791

IS - 4

ER -