Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Relationship between DNA damage, rejoining and cell killing by radiation in mammalian cells.
AU - Nunez, M. I.
AU - McMillan, T. J.
AU - Valenzuela, M. T.
AU - Ruiz de Almodovar, J. M.
AU - Pedraza, V.
PY - 1996/5
Y1 - 1996/5
N2 - The prevailing hypothesis on the mechanism of radiation-induced cell killing identifies the genetic material deoxyribonucleic acid (DNA) as the most important subcellular target at biologically relevant doses. In this review we present new data and summarize the role of the DNA double-strand breaks (dsb) induced by ionizing radiation and DNA dsb rejoining as determinants of cellular radiosensitivity. When cells were irradiated at high dose-rate, two molecular end-points were identified which often correlated with radiosensitivity: (1) the apparent number of DNA dsb induced per Gy per DNA unit and (2) the half-time of the fast component of the DNA dsb rejoining kinetics. These two molecular determinants, not mutually exclusive, may be linked through a common factor such as the conformation of DNA.
AB - The prevailing hypothesis on the mechanism of radiation-induced cell killing identifies the genetic material deoxyribonucleic acid (DNA) as the most important subcellular target at biologically relevant doses. In this review we present new data and summarize the role of the DNA double-strand breaks (dsb) induced by ionizing radiation and DNA dsb rejoining as determinants of cellular radiosensitivity. When cells were irradiated at high dose-rate, two molecular end-points were identified which often correlated with radiosensitivity: (1) the apparent number of DNA dsb induced per Gy per DNA unit and (2) the half-time of the fast component of the DNA dsb rejoining kinetics. These two molecular determinants, not mutually exclusive, may be linked through a common factor such as the conformation of DNA.
KW - Author Keywords
KW - Radiosensitivity
KW - DNA double-strand breaks
KW - Initial damage
KW - Rejoining
U2 - 10.1016/0167-8140(96)01732-X
DO - 10.1016/0167-8140(96)01732-X
M3 - Journal article
VL - 39
SP - 155
EP - 165
JO - Radiotherapy and Oncology
JF - Radiotherapy and Oncology
SN - 1879-0887
IS - 2
ER -