Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Response of female Lutzomyia longipalpis to host odour kairomones from human skin
AU - Rebollar-Tellez, E. A.
AU - Hamilton, J. G.C.
AU - Ward, R. D.
PY - 1999/9/1
Y1 - 1999/9/1
N2 - Lutzomyia longipalpis Lute and Neiva (Diptera: Psychodidae) is the vector of Leishmania chagasi, the aetiologic agent of visceral leishmaniasis in the New World. In the present study, the response of female sandflies from Jacobina, Brazil, to human odours from six different volunteers was investigated. Glass Petri dishes were handled by different volunteers and then exposed to female sand flies. There was a significant difference between subjects in that some individuals were more attractive or less repellent to sandflies. Response of flies to handled Petri dishes was higher during the first minutes of observation, suggesting the presence of volatile compounds in hand odours. Extracts of glass Petri dishes that had been handled by the volunteers were made with organic solvents such as acetone, methanol, pentane and ether. These were then concentrated and tested for sandfly response. Only extracts carried out with non-polar solvents such as pentane and ether were able to transfer odours from handled glass Petri dishes onto clean dishes. The attractivity of male and female human subjects was monitored for 80 days, and minor fluctuations in attractiveness were observed.
AB - Lutzomyia longipalpis Lute and Neiva (Diptera: Psychodidae) is the vector of Leishmania chagasi, the aetiologic agent of visceral leishmaniasis in the New World. In the present study, the response of female sandflies from Jacobina, Brazil, to human odours from six different volunteers was investigated. Glass Petri dishes were handled by different volunteers and then exposed to female sand flies. There was a significant difference between subjects in that some individuals were more attractive or less repellent to sandflies. Response of flies to handled Petri dishes was higher during the first minutes of observation, suggesting the presence of volatile compounds in hand odours. Extracts of glass Petri dishes that had been handled by the volunteers were made with organic solvents such as acetone, methanol, pentane and ether. These were then concentrated and tested for sandfly response. Only extracts carried out with non-polar solvents such as pentane and ether were able to transfer odours from handled glass Petri dishes onto clean dishes. The attractivity of male and female human subjects was monitored for 80 days, and minor fluctuations in attractiveness were observed.
KW - Attractant
KW - Host-preferences
KW - Human skin odours
KW - Kairomones
KW - Lutzomyia longipalpis
KW - Non-polar compounds
KW - Sandflies
U2 - 10.1046/j.1365-3032.1999.00133.x
DO - 10.1046/j.1365-3032.1999.00133.x
M3 - Journal article
AN - SCOPUS:0032873843
VL - 24
SP - 220
EP - 226
JO - Physiological Entomology
JF - Physiological Entomology
SN - 0307-6962
IS - 3
ER -