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  • IJPara16_030R1

    Rights statement: This is the author’s version of a work that was accepted for publication in International Journal for Parasitology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in International Journal for Parasitology, 46 (10), 2016 DOI: 10.1016/j.ijpara.2016.04.006

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Roles of the Nfu Fe-S targeting factors in the trypanosome mitochondrion

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Roles of the Nfu Fe-S targeting factors in the trypanosome mitochondrion. / Benz, Corinna; Kovárová, Julie; Králová-Hromadová, Ivica et al.
In: International Journal for Parasitology, Vol. 46, No. 10, 09.2016, p. 641-651.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Benz, C, Kovárová, J, Králová-Hromadová, I, Pierlik, AJ & Lukeš, J 2016, 'Roles of the Nfu Fe-S targeting factors in the trypanosome mitochondrion', International Journal for Parasitology, vol. 46, no. 10, pp. 641-651. https://doi.org/10.1016/j.ijpara.2016.04.006

APA

Benz, C., Kovárová, J., Králová-Hromadová, I., Pierlik, A. J., & Lukeš, J. (2016). Roles of the Nfu Fe-S targeting factors in the trypanosome mitochondrion. International Journal for Parasitology, 46(10), 641-651. https://doi.org/10.1016/j.ijpara.2016.04.006

Vancouver

Benz C, Kovárová J, Králová-Hromadová I, Pierlik AJ, Lukeš J. Roles of the Nfu Fe-S targeting factors in the trypanosome mitochondrion. International Journal for Parasitology. 2016 Sept;46(10):641-651. Epub 2016 May 12. doi: 10.1016/j.ijpara.2016.04.006

Author

Benz, Corinna ; Kovárová, Julie ; Králová-Hromadová, Ivica et al. / Roles of the Nfu Fe-S targeting factors in the trypanosome mitochondrion. In: International Journal for Parasitology. 2016 ; Vol. 46, No. 10. pp. 641-651.

Bibtex

@article{a6fda3292421486a88183ee8224bd176,
title = "Roles of the Nfu Fe-S targeting factors in the trypanosome mitochondrion",
abstract = "Iron–sulphur clusters (ISCs) are protein co-factors essential for a wide range of cellular functions. The core iron–sulphur cluster assembly machinery resides in the mitochondrion, yet due to export of an essential precursor from the organelle, it is also needed for cytosolic and nuclear iron–sulphur cluster assembly. In mitochondria all [4Fe–4S] iron–sulphur clusters are synthesised and transferred to specific apoproteins by so-called iron–sulphur cluster targeting factors. One of these factors is the universally present mitochondrial Nfu1, which in humans is required for the proper assembly of a subset of mitochondrial [4Fe–4S] proteins. Although most eukaryotes harbour a single Nfu1, the genomes of Trypanosoma brucei and related flagellates encode three Nfu genes. All three Nfu proteins localise to the mitochondrion in the procyclic form of T. brucei, and TbNfu2 and TbNfu3 are both individually essential for growth in bloodstream and procyclic forms, suggesting highly specific functions for each of these proteins in the trypanosome cell. Moreover, these two proteins are functional in the iron–sulphur cluster assembly in a heterologous system and rescue the growth defect of a yeast deletion mutant.",
keywords = "Trypanosoma brucei, Nfu1, Iron–sulphur cluster, Fe–S, Mitochondrion",
author = "Corinna Benz and Julie Kov{\'a}rov{\'a} and Ivica Kr{\'a}lov{\'a}-Hromadov{\'a} and Pierlik, {Antonio J.} and Julius Luke{\v s}",
note = "This is the author{\textquoteright}s version of a work that was accepted for publication in International Journal for Parasitology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in International Journal for Parasitology, 46 (10), 2016 DOI: 10.1016/j.ijpara.2016.04.006",
year = "2016",
month = sep,
doi = "10.1016/j.ijpara.2016.04.006",
language = "English",
volume = "46",
pages = "641--651",
journal = "International Journal for Parasitology",
issn = "0020-7519",
publisher = "Elsevier Limited",
number = "10",

}

RIS

TY - JOUR

T1 - Roles of the Nfu Fe-S targeting factors in the trypanosome mitochondrion

AU - Benz, Corinna

AU - Kovárová, Julie

AU - Králová-Hromadová, Ivica

AU - Pierlik, Antonio J.

AU - Lukeš, Julius

N1 - This is the author’s version of a work that was accepted for publication in International Journal for Parasitology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in International Journal for Parasitology, 46 (10), 2016 DOI: 10.1016/j.ijpara.2016.04.006

PY - 2016/9

Y1 - 2016/9

N2 - Iron–sulphur clusters (ISCs) are protein co-factors essential for a wide range of cellular functions. The core iron–sulphur cluster assembly machinery resides in the mitochondrion, yet due to export of an essential precursor from the organelle, it is also needed for cytosolic and nuclear iron–sulphur cluster assembly. In mitochondria all [4Fe–4S] iron–sulphur clusters are synthesised and transferred to specific apoproteins by so-called iron–sulphur cluster targeting factors. One of these factors is the universally present mitochondrial Nfu1, which in humans is required for the proper assembly of a subset of mitochondrial [4Fe–4S] proteins. Although most eukaryotes harbour a single Nfu1, the genomes of Trypanosoma brucei and related flagellates encode three Nfu genes. All three Nfu proteins localise to the mitochondrion in the procyclic form of T. brucei, and TbNfu2 and TbNfu3 are both individually essential for growth in bloodstream and procyclic forms, suggesting highly specific functions for each of these proteins in the trypanosome cell. Moreover, these two proteins are functional in the iron–sulphur cluster assembly in a heterologous system and rescue the growth defect of a yeast deletion mutant.

AB - Iron–sulphur clusters (ISCs) are protein co-factors essential for a wide range of cellular functions. The core iron–sulphur cluster assembly machinery resides in the mitochondrion, yet due to export of an essential precursor from the organelle, it is also needed for cytosolic and nuclear iron–sulphur cluster assembly. In mitochondria all [4Fe–4S] iron–sulphur clusters are synthesised and transferred to specific apoproteins by so-called iron–sulphur cluster targeting factors. One of these factors is the universally present mitochondrial Nfu1, which in humans is required for the proper assembly of a subset of mitochondrial [4Fe–4S] proteins. Although most eukaryotes harbour a single Nfu1, the genomes of Trypanosoma brucei and related flagellates encode three Nfu genes. All three Nfu proteins localise to the mitochondrion in the procyclic form of T. brucei, and TbNfu2 and TbNfu3 are both individually essential for growth in bloodstream and procyclic forms, suggesting highly specific functions for each of these proteins in the trypanosome cell. Moreover, these two proteins are functional in the iron–sulphur cluster assembly in a heterologous system and rescue the growth defect of a yeast deletion mutant.

KW - Trypanosoma brucei

KW - Nfu1

KW - Iron–sulphur cluster

KW - Fe–S

KW - Mitochondrion

U2 - 10.1016/j.ijpara.2016.04.006

DO - 10.1016/j.ijpara.2016.04.006

M3 - Journal article

VL - 46

SP - 641

EP - 651

JO - International Journal for Parasitology

JF - International Journal for Parasitology

SN - 0020-7519

IS - 10

ER -