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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Root ABA Accumulation Delays Lateral Root Emergence in Osmotically Stressed Barley Plants by Decreasing Root Primordial IAA Accumulation
AU - Akhiyarova, Guzel
AU - Veselov, Dmitriy
AU - Ivanov, Ruslan
AU - Sharipova, Guzel
AU - Ivanov, Igor
AU - Dodd, Ian C.
AU - Kudoyarova, Guzel
PY - 2023/1/9
Y1 - 2023/1/9
N2 - Increased auxin levels in root primordia are important in controlling root branching, while their interaction with abscisic acid (ABA) likely regulates lateral root development in water-deficient plants. The role of ABA accumulation in regulating root branching was investigated using immunolocalization to detect auxin (indoleacetic acid, IAA) and ABA (abscisic acid) in root primordia of the ABA-deficient barley mutant Az34 and its parental genotype (cv. Steptoe) barley plants. Osmotic stress strongly inhibited lateral root branching in Steptoe plants, but hardly affected Az34. Root primordial cells of Steptoe plants had increased immunostaining for ABA but diminished staining for IAA. ABA did not accumulate in root primordia of the Az34, and IAA levels and distribution were unaltered. Treating Az34 plants with exogenous ABA decreased root IAA concentration, while increasing root primordial ABA accumulation and decreasing root primordial IAA concentration. Although ABA treatment of Az34 plants increased the root primordial number, it decreased the number of visible emerged lateral roots. These effects were qualitatively similar to that of osmotic stress on the number of lateral root primordia and emerged lateral roots in Steptoe. Thus ABA accumulation (and its crosstalk with auxin) in root primordia seems important in regulating lateral root branching in response to water stress.
AB - Increased auxin levels in root primordia are important in controlling root branching, while their interaction with abscisic acid (ABA) likely regulates lateral root development in water-deficient plants. The role of ABA accumulation in regulating root branching was investigated using immunolocalization to detect auxin (indoleacetic acid, IAA) and ABA (abscisic acid) in root primordia of the ABA-deficient barley mutant Az34 and its parental genotype (cv. Steptoe) barley plants. Osmotic stress strongly inhibited lateral root branching in Steptoe plants, but hardly affected Az34. Root primordial cells of Steptoe plants had increased immunostaining for ABA but diminished staining for IAA. ABA did not accumulate in root primordia of the Az34, and IAA levels and distribution were unaltered. Treating Az34 plants with exogenous ABA decreased root IAA concentration, while increasing root primordial ABA accumulation and decreasing root primordial IAA concentration. Although ABA treatment of Az34 plants increased the root primordial number, it decreased the number of visible emerged lateral roots. These effects were qualitatively similar to that of osmotic stress on the number of lateral root primordia and emerged lateral roots in Steptoe. Thus ABA accumulation (and its crosstalk with auxin) in root primordia seems important in regulating lateral root branching in response to water stress.
KW - Hordeum vulgare L.
KW - ABA-deficient mutant Az34
KW - hormones
KW - immunolocalization
KW - primordia
KW - osmotic stress
U2 - 10.3390/ijpb14010007
DO - 10.3390/ijpb14010007
M3 - Journal article
VL - 14
SP - 77
EP - 90
JO - International Journal of Plant Biology
JF - International Journal of Plant Biology
SN - 2037-0164
IS - 1
ER -