Rights statement: This is the peer reviewed version of the following article: Atkinson, N., Leitão, N., Orr, D. J., Meyer, M. T., Carmo-Silva, E., Griffiths, H., Smith, A. M. and McCormick, A. J. (2017), Rubisco small subunits from the unicellular green alga Chlamydomonas complement Rubisco-deficient mutants of Arabidopsis. New Phytol, 214: 655–667. doi:10.1111/nph.14414 which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/nph.14414/abstract This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Rubisco small subunits from the unicellular green alga Chlamydomonas complement Rubisco-deficient mutants of Arabidopsis
AU - Atkinson, Nicky
AU - Leitão, Nuno
AU - Orr, Douglas John
AU - Meyer, Moritz
AU - Carmo-Silva, Ana Elizabete
AU - Griffiths, Howard
AU - Smith, Alison
AU - McCormick, Alistair
N1 - This is the peer reviewed version of the following article: Atkinson, N., Leitão, N., Orr, D. J., Meyer, M. T., Carmo-Silva, E., Griffiths, H., Smith, A. M. and McCormick, A. J. (2017), Rubisco small subunits from the unicellular green alga Chlamydomonas complement Rubisco-deficient mutants of Arabidopsis. New Phytol, 214: 655–667. doi:10.1111/nph.14414 which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/nph.14414/abstract This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
PY - 2017/4
Y1 - 2017/4
N2 - Introducing components of algal carbon concentrating mechanisms (CCMs) into higher plant chloroplasts could increase photosynthetic productivity. A key component is the Rubisco-containing pyrenoid that is needed to minimise CO2 retro-diffusion for CCM operating efficiency.Rubisco in Arabidopsis was re-engineered to incorporate sequence elements that are thought to be essential for recruitment of Rubisco to the pyrenoid, namely the algal Rubisco small subunit (SSU, encoded by rbcS) or only the surface-exposed algal SSU α-helices.Leaves of Arabidopsis rbcs mutants expressing ‘pyrenoid-competent’ chimeric Arabidopsis SSUs containing the SSU α-helices from Chlamydomonas reinhardtii can form hybrid Rubisco complexes with catalytic properties similar to those of native Rubisco, suggesting that the α-helices are catalytically neutral.The growth and photosynthetic performance of complemented Arabidopsis rbcs mutants producing near wild-type levels of the hybrid Rubisco were similar to those of wild-type controls. Arabidopsis rbcs mutants expressing a Chlamydomonas SSU differed from wild-type plants with respect to Rubisco catalysis, photosynthesis and growth. This confirms a role for the SSU in influencing Rubisco catalytic properties.
AB - Introducing components of algal carbon concentrating mechanisms (CCMs) into higher plant chloroplasts could increase photosynthetic productivity. A key component is the Rubisco-containing pyrenoid that is needed to minimise CO2 retro-diffusion for CCM operating efficiency.Rubisco in Arabidopsis was re-engineered to incorporate sequence elements that are thought to be essential for recruitment of Rubisco to the pyrenoid, namely the algal Rubisco small subunit (SSU, encoded by rbcS) or only the surface-exposed algal SSU α-helices.Leaves of Arabidopsis rbcs mutants expressing ‘pyrenoid-competent’ chimeric Arabidopsis SSUs containing the SSU α-helices from Chlamydomonas reinhardtii can form hybrid Rubisco complexes with catalytic properties similar to those of native Rubisco, suggesting that the α-helices are catalytically neutral.The growth and photosynthetic performance of complemented Arabidopsis rbcs mutants producing near wild-type levels of the hybrid Rubisco were similar to those of wild-type controls. Arabidopsis rbcs mutants expressing a Chlamydomonas SSU differed from wild-type plants with respect to Rubisco catalysis, photosynthesis and growth. This confirms a role for the SSU in influencing Rubisco catalytic properties.
KW - Arabidopsis thaliana
KW - carbon concentrating mechanism (CCM)
KW - Chlamydomonas reinhardtii
KW - chloroplast
KW - photosynthesis
KW - pyrenoid
KW - Rubisco
KW - tobacco
U2 - 10.1111/nph.14414
DO - 10.1111/nph.14414
M3 - Journal article
VL - 214
SP - 655
EP - 667
JO - New Phytologist
JF - New Phytologist
SN - 0028-646X
IS - 2
ER -