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    Rights statement: This is the peer reviewed version of the following article: Atkinson, N., Leitão, N., Orr, D. J., Meyer, M. T., Carmo-Silva, E., Griffiths, H., Smith, A. M. and McCormick, A. J. (2017), Rubisco small subunits from the unicellular green alga Chlamydomonas complement Rubisco-deficient mutants of Arabidopsis. New Phytol, 214: 655–667. doi:10.1111/nph.14414 which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/nph.14414/abstract This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.

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Rubisco small subunits from the unicellular green alga Chlamydomonas complement Rubisco-deficient mutants of Arabidopsis

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Rubisco small subunits from the unicellular green alga Chlamydomonas complement Rubisco-deficient mutants of Arabidopsis. / Atkinson, Nicky; Leitão, Nuno; Orr, Douglas John et al.
In: New Phytologist, Vol. 214, No. 2, 04.2017, p. 655-667.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Atkinson, N, Leitão, N, Orr, DJ, Meyer, M, Carmo-Silva, AE, Griffiths, H, Smith, A & McCormick, A 2017, 'Rubisco small subunits from the unicellular green alga Chlamydomonas complement Rubisco-deficient mutants of Arabidopsis', New Phytologist, vol. 214, no. 2, pp. 655-667. https://doi.org/10.1111/nph.14414

APA

Atkinson, N., Leitão, N., Orr, D. J., Meyer, M., Carmo-Silva, A. E., Griffiths, H., Smith, A., & McCormick, A. (2017). Rubisco small subunits from the unicellular green alga Chlamydomonas complement Rubisco-deficient mutants of Arabidopsis. New Phytologist, 214(2), 655-667. https://doi.org/10.1111/nph.14414

Vancouver

Atkinson N, Leitão N, Orr DJ, Meyer M, Carmo-Silva AE, Griffiths H et al. Rubisco small subunits from the unicellular green alga Chlamydomonas complement Rubisco-deficient mutants of Arabidopsis. New Phytologist. 2017 Apr;214(2):655-667. Epub 2017 Jan 13. doi: 10.1111/nph.14414

Author

Atkinson, Nicky ; Leitão, Nuno ; Orr, Douglas John et al. / Rubisco small subunits from the unicellular green alga Chlamydomonas complement Rubisco-deficient mutants of Arabidopsis. In: New Phytologist. 2017 ; Vol. 214, No. 2. pp. 655-667.

Bibtex

@article{03b1f63b8f0d46dfb3eed23444fefb18,
title = "Rubisco small subunits from the unicellular green alga Chlamydomonas complement Rubisco-deficient mutants of Arabidopsis",
abstract = "Introducing components of algal carbon concentrating mechanisms (CCMs) into higher plant chloroplasts could increase photosynthetic productivity. A key component is the Rubisco-containing pyrenoid that is needed to minimise CO2 retro-diffusion for CCM operating efficiency.Rubisco in Arabidopsis was re-engineered to incorporate sequence elements that are thought to be essential for recruitment of Rubisco to the pyrenoid, namely the algal Rubisco small subunit (SSU, encoded by rbcS) or only the surface-exposed algal SSU α-helices.Leaves of Arabidopsis rbcs mutants expressing {\textquoteleft}pyrenoid-competent{\textquoteright} chimeric Arabidopsis SSUs containing the SSU α-helices from Chlamydomonas reinhardtii can form hybrid Rubisco complexes with catalytic properties similar to those of native Rubisco, suggesting that the α-helices are catalytically neutral.The growth and photosynthetic performance of complemented Arabidopsis rbcs mutants producing near wild-type levels of the hybrid Rubisco were similar to those of wild-type controls. Arabidopsis rbcs mutants expressing a Chlamydomonas SSU differed from wild-type plants with respect to Rubisco catalysis, photosynthesis and growth. This confirms a role for the SSU in influencing Rubisco catalytic properties.",
keywords = "Arabidopsis thaliana , carbon concentrating mechanism (CCM), Chlamydomonas reinhardtii , chloroplast, photosynthesis, pyrenoid, Rubisco, tobacco",
author = "Nicky Atkinson and Nuno Leit{\~a}o and Orr, {Douglas John} and Moritz Meyer and Carmo-Silva, {Ana Elizabete} and Howard Griffiths and Alison Smith and Alistair McCormick",
note = "This is the peer reviewed version of the following article: Atkinson, N., Leit{\~a}o, N., Orr, D. J., Meyer, M. T., Carmo-Silva, E., Griffiths, H., Smith, A. M. and McCormick, A. J. (2017), Rubisco small subunits from the unicellular green alga Chlamydomonas complement Rubisco-deficient mutants of Arabidopsis. New Phytol, 214: 655–667. doi:10.1111/nph.14414 which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/nph.14414/abstract This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.",
year = "2017",
month = apr,
doi = "10.1111/nph.14414",
language = "English",
volume = "214",
pages = "655--667",
journal = "New Phytologist",
issn = "0028-646X",
publisher = "Wiley",
number = "2",

}

RIS

TY - JOUR

T1 - Rubisco small subunits from the unicellular green alga Chlamydomonas complement Rubisco-deficient mutants of Arabidopsis

AU - Atkinson, Nicky

AU - Leitão, Nuno

AU - Orr, Douglas John

AU - Meyer, Moritz

AU - Carmo-Silva, Ana Elizabete

AU - Griffiths, Howard

AU - Smith, Alison

AU - McCormick, Alistair

N1 - This is the peer reviewed version of the following article: Atkinson, N., Leitão, N., Orr, D. J., Meyer, M. T., Carmo-Silva, E., Griffiths, H., Smith, A. M. and McCormick, A. J. (2017), Rubisco small subunits from the unicellular green alga Chlamydomonas complement Rubisco-deficient mutants of Arabidopsis. New Phytol, 214: 655–667. doi:10.1111/nph.14414 which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/nph.14414/abstract This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.

PY - 2017/4

Y1 - 2017/4

N2 - Introducing components of algal carbon concentrating mechanisms (CCMs) into higher plant chloroplasts could increase photosynthetic productivity. A key component is the Rubisco-containing pyrenoid that is needed to minimise CO2 retro-diffusion for CCM operating efficiency.Rubisco in Arabidopsis was re-engineered to incorporate sequence elements that are thought to be essential for recruitment of Rubisco to the pyrenoid, namely the algal Rubisco small subunit (SSU, encoded by rbcS) or only the surface-exposed algal SSU α-helices.Leaves of Arabidopsis rbcs mutants expressing ‘pyrenoid-competent’ chimeric Arabidopsis SSUs containing the SSU α-helices from Chlamydomonas reinhardtii can form hybrid Rubisco complexes with catalytic properties similar to those of native Rubisco, suggesting that the α-helices are catalytically neutral.The growth and photosynthetic performance of complemented Arabidopsis rbcs mutants producing near wild-type levels of the hybrid Rubisco were similar to those of wild-type controls. Arabidopsis rbcs mutants expressing a Chlamydomonas SSU differed from wild-type plants with respect to Rubisco catalysis, photosynthesis and growth. This confirms a role for the SSU in influencing Rubisco catalytic properties.

AB - Introducing components of algal carbon concentrating mechanisms (CCMs) into higher plant chloroplasts could increase photosynthetic productivity. A key component is the Rubisco-containing pyrenoid that is needed to minimise CO2 retro-diffusion for CCM operating efficiency.Rubisco in Arabidopsis was re-engineered to incorporate sequence elements that are thought to be essential for recruitment of Rubisco to the pyrenoid, namely the algal Rubisco small subunit (SSU, encoded by rbcS) or only the surface-exposed algal SSU α-helices.Leaves of Arabidopsis rbcs mutants expressing ‘pyrenoid-competent’ chimeric Arabidopsis SSUs containing the SSU α-helices from Chlamydomonas reinhardtii can form hybrid Rubisco complexes with catalytic properties similar to those of native Rubisco, suggesting that the α-helices are catalytically neutral.The growth and photosynthetic performance of complemented Arabidopsis rbcs mutants producing near wild-type levels of the hybrid Rubisco were similar to those of wild-type controls. Arabidopsis rbcs mutants expressing a Chlamydomonas SSU differed from wild-type plants with respect to Rubisco catalysis, photosynthesis and growth. This confirms a role for the SSU in influencing Rubisco catalytic properties.

KW - Arabidopsis thaliana

KW - carbon concentrating mechanism (CCM)

KW - Chlamydomonas reinhardtii

KW - chloroplast

KW - photosynthesis

KW - pyrenoid

KW - Rubisco

KW - tobacco

U2 - 10.1111/nph.14414

DO - 10.1111/nph.14414

M3 - Journal article

VL - 214

SP - 655

EP - 667

JO - New Phytologist

JF - New Phytologist

SN - 0028-646X

IS - 2

ER -