TY - JOUR

T1 - Salt-induced oxidative stress in rosemary plants

T2 - Damage or protection?

AU - Tounekti, Taieb

AU - Vadel, Ahmedou M.

AU - Onate Gutierrez, Marta

AU - Khemira, Habib

AU - Munne-Bosch, Sergi

PY - 2011/6

Y1 - 2011/6

N2 - Mechanisms of photoprotection and antioxidant protection, including changes in chlorophylls, xanthophyll cycle components and levels of low-molecular-weight chloroplastic antioxidants (lutein, beta-carotene and alpha-tocopherol) were studied together with levels of malondialdehyde, a product of lipid peroxidation, in the response of rosemary (Rosmarinus officinalis L) plants to salt stress. Plants were exposed to increasing NaCl concentrations (50, 100 and 150 mM) for 6 weeks, and two concentrations of the following chloride salts: KCl, CaCl(2), MgCl(2) and FeCl(3), were used together with 100 mM NaCl to explore the extent to which these salts can alter the mechanisms of photoprotection, antioxidant protection and malondialdehyde accumulation in leaves. Increasing concentrations of NaCl decreased leaf water contents and photosynthetic pigment levels, while the contents of alpha-tocopherol and malondialdehyde increased. but with completely different kinetics. alpha-Tocopherol levels increased in a dose-dependent manner as stress progressed, while malondialdehyde levels increased at the highest dose (150 mM NaCl) but only during early phases of stress. Furthermore, although the addition of chloride salts to NaCl-treated plants apparently improved leaf physiological status, in terms of water and chlorophyll contents, plants showed an increased photoprotective demand and increased oxidative stress, particularly in FeCl(3)-treated plants. It is concluded that (i) rosemary plants can withstand moderate doses of NaCl in the medium (at least 150 mM NaCl for 6 weeks), (ii) oxidative stress may be a mechanism for protecting plants from moderate doses of salt stress rather than causing damage to plants, and (iii) the addition of chloride salts to NaCl-treated plants may dramatically increase the photoprotective demand and oxidative stress of leaves, while plant growth is not negatively affected. (C) 2010 Elsevier B.V. All rights reserved.

AB - Mechanisms of photoprotection and antioxidant protection, including changes in chlorophylls, xanthophyll cycle components and levels of low-molecular-weight chloroplastic antioxidants (lutein, beta-carotene and alpha-tocopherol) were studied together with levels of malondialdehyde, a product of lipid peroxidation, in the response of rosemary (Rosmarinus officinalis L) plants to salt stress. Plants were exposed to increasing NaCl concentrations (50, 100 and 150 mM) for 6 weeks, and two concentrations of the following chloride salts: KCl, CaCl(2), MgCl(2) and FeCl(3), were used together with 100 mM NaCl to explore the extent to which these salts can alter the mechanisms of photoprotection, antioxidant protection and malondialdehyde accumulation in leaves. Increasing concentrations of NaCl decreased leaf water contents and photosynthetic pigment levels, while the contents of alpha-tocopherol and malondialdehyde increased. but with completely different kinetics. alpha-Tocopherol levels increased in a dose-dependent manner as stress progressed, while malondialdehyde levels increased at the highest dose (150 mM NaCl) but only during early phases of stress. Furthermore, although the addition of chloride salts to NaCl-treated plants apparently improved leaf physiological status, in terms of water and chlorophyll contents, plants showed an increased photoprotective demand and increased oxidative stress, particularly in FeCl(3)-treated plants. It is concluded that (i) rosemary plants can withstand moderate doses of NaCl in the medium (at least 150 mM NaCl for 6 weeks), (ii) oxidative stress may be a mechanism for protecting plants from moderate doses of salt stress rather than causing damage to plants, and (iii) the addition of chloride salts to NaCl-treated plants may dramatically increase the photoprotective demand and oxidative stress of leaves, while plant growth is not negatively affected. (C) 2010 Elsevier B.V. All rights reserved.

KW - Antioxidants

KW - Carotenoids

KW - Chloroplasts

KW - Ionic interactions

KW - Photoprotection

KW - Salt stress

KW - Tocopherols

KW - Xanthophyll cycle

KW - LEAF WATER RELATIONS

KW - ALPHA-TOCOPHEROL

KW - SALINITY TOLERANCE

KW - LIPID-PEROXIDATION

KW - CO2 ASSIMILATION

KW - CALCIUM-CHLORIDE

KW - CITRUS PLANTS

KW - GAS-EXCHANGE

KW - ION CONTENT

KW - LEAVES

U2 - 10.1016/j.envexpbot.2010.12.016

DO - 10.1016/j.envexpbot.2010.12.016

M3 - Journal article

VL - 71

SP - 298

EP - 305

JO - Environmental and Experimental Botany

JF - Environmental and Experimental Botany

SN - 0098-8472

IS - 2

ER -