Home > Research > Publications & Outputs > Separating and characterizing functional alkane...

Electronic data

  • 1-s2.0-S0923250816300778-main

    Rights statement: This is the author’s version of a work that was accepted for publication in Research in Microbiology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Research in Microbiology, 167, 9-10, 2016 DOI: 10.1016/j.resmic.2016.07.004

    Accepted author manuscript, 933 KB, PDF document

    Available under license: CC BY-NC-ND: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License

Links

Text available via DOI:

View graph of relations

Separating and characterizing functional alkane degraders from crude-oil-contaminated sites via magnetic nanoparticle-mediated isolation

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Published

Standard

Separating and characterizing functional alkane degraders from crude-oil-contaminated sites via magnetic nanoparticle-mediated isolation. / Wang, Xinzi; Zhao, Xiaohui; Li, Hangbing et al.

In: Research in Microbiology, Vol. 167, No. 9-10, 11.2016, p. 731-744.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

APA

Vancouver

Wang X, Zhao X, Li H, Jia J, Liu Y, Ejenavi O et al. Separating and characterizing functional alkane degraders from crude-oil-contaminated sites via magnetic nanoparticle-mediated isolation. Research in Microbiology. 2016 Nov;167(9-10):731-744. Epub 2016 Jul 27. doi: 10.1016/j.resmic.2016.07.004

Author

Bibtex

@article{eea2ddc0f1a742df989ae5cd4d4afda6,
title = "Separating and characterizing functional alkane degraders from crude-oil-contaminated sites via magnetic nanoparticle-mediated isolation",
abstract = "Uncultivable microorganisms account for over 99% of all species on the planet, but their functions are yet not well characterized. Though many cultivable degraders for n-alkanes have been intensively investigated, the roles of functional n-alkane degraders remain hidden in the natural environment. This study introduces the novel magnetic nanoparticle-mediated isolation (MMI) technology in Nigerian soils and successfully separates functional microbes belonging to the families Oxalobacteraceae and Moraxellaceae, which were dominant and responsible for alkane metabolism in situ. The alkR-type n-alkane monooxygenase genes, instead of alkA- or alkP-type, were the key functional genes involved in the n-alkane degradation process. Further physiological investigation via a BIOLOG PM plate revealed some carbon (Tween 20, Tween 40 and Tween 80) and nitrogen (tyramine, L-glutamine and D-aspartic acid) sources promoting microbial respiration and n-alkane degradation. With further addition of promoter carbon or nitrogen sources, the separated functional alkane degraders significantly improved n-alkane biodegradation rates. This suggests that MMI is a promising technology for separating functional microbes from complex microbiota, with deeper insight into their ecological functions and influencing factors. The technique also broadens the application of the BIOLOG PM plate for physiological research on functional yet uncultivable microorganisms.",
keywords = "n-Alkane, Soil, Biodegradation, Magnetic nanoparticle-mediated isolation, Uncultivable microorganisms, Functional alkane degraders, BIOLOG PM plate",
author = "Xinzi Wang and Xiaohui Zhao and Hangbing Li and Jianli Jia and Yueqiao Liu and Odafe Ejenavi and Aizhong Ding and Yujiao Sun and Dayi Zhang",
note = "This is the author{\textquoteright}s version of a work that was accepted for publication in Research in Microbiology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Research in Microbiology, 167, 9-10, 2016 DOI: 10.1016/j.resmic.2016.07.004",
year = "2016",
month = nov,
doi = "10.1016/j.resmic.2016.07.004",
language = "English",
volume = "167",
pages = "731--744",
journal = "Research in Microbiology",
issn = "0923-2508",
publisher = "Elsevier Masson SAS",
number = "9-10",

}

RIS

TY - JOUR

T1 - Separating and characterizing functional alkane degraders from crude-oil-contaminated sites via magnetic nanoparticle-mediated isolation

AU - Wang, Xinzi

AU - Zhao, Xiaohui

AU - Li, Hangbing

AU - Jia, Jianli

AU - Liu, Yueqiao

AU - Ejenavi, Odafe

AU - Ding, Aizhong

AU - Sun, Yujiao

AU - Zhang, Dayi

N1 - This is the author’s version of a work that was accepted for publication in Research in Microbiology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Research in Microbiology, 167, 9-10, 2016 DOI: 10.1016/j.resmic.2016.07.004

PY - 2016/11

Y1 - 2016/11

N2 - Uncultivable microorganisms account for over 99% of all species on the planet, but their functions are yet not well characterized. Though many cultivable degraders for n-alkanes have been intensively investigated, the roles of functional n-alkane degraders remain hidden in the natural environment. This study introduces the novel magnetic nanoparticle-mediated isolation (MMI) technology in Nigerian soils and successfully separates functional microbes belonging to the families Oxalobacteraceae and Moraxellaceae, which were dominant and responsible for alkane metabolism in situ. The alkR-type n-alkane monooxygenase genes, instead of alkA- or alkP-type, were the key functional genes involved in the n-alkane degradation process. Further physiological investigation via a BIOLOG PM plate revealed some carbon (Tween 20, Tween 40 and Tween 80) and nitrogen (tyramine, L-glutamine and D-aspartic acid) sources promoting microbial respiration and n-alkane degradation. With further addition of promoter carbon or nitrogen sources, the separated functional alkane degraders significantly improved n-alkane biodegradation rates. This suggests that MMI is a promising technology for separating functional microbes from complex microbiota, with deeper insight into their ecological functions and influencing factors. The technique also broadens the application of the BIOLOG PM plate for physiological research on functional yet uncultivable microorganisms.

AB - Uncultivable microorganisms account for over 99% of all species on the planet, but their functions are yet not well characterized. Though many cultivable degraders for n-alkanes have been intensively investigated, the roles of functional n-alkane degraders remain hidden in the natural environment. This study introduces the novel magnetic nanoparticle-mediated isolation (MMI) technology in Nigerian soils and successfully separates functional microbes belonging to the families Oxalobacteraceae and Moraxellaceae, which were dominant and responsible for alkane metabolism in situ. The alkR-type n-alkane monooxygenase genes, instead of alkA- or alkP-type, were the key functional genes involved in the n-alkane degradation process. Further physiological investigation via a BIOLOG PM plate revealed some carbon (Tween 20, Tween 40 and Tween 80) and nitrogen (tyramine, L-glutamine and D-aspartic acid) sources promoting microbial respiration and n-alkane degradation. With further addition of promoter carbon or nitrogen sources, the separated functional alkane degraders significantly improved n-alkane biodegradation rates. This suggests that MMI is a promising technology for separating functional microbes from complex microbiota, with deeper insight into their ecological functions and influencing factors. The technique also broadens the application of the BIOLOG PM plate for physiological research on functional yet uncultivable microorganisms.

KW - n-Alkane

KW - Soil

KW - Biodegradation

KW - Magnetic nanoparticle-mediated isolation

KW - Uncultivable microorganisms

KW - Functional alkane degraders

KW - BIOLOG PM plate

U2 - 10.1016/j.resmic.2016.07.004

DO - 10.1016/j.resmic.2016.07.004

M3 - Journal article

VL - 167

SP - 731

EP - 744

JO - Research in Microbiology

JF - Research in Microbiology

SN - 0923-2508

IS - 9-10

ER -