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Separation of natural populations of coliform bacteria from freshwater and sewage by magnetic-bead cell sorting

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Separation of natural populations of coliform bacteria from freshwater and sewage by magnetic-bead cell sorting. / Porter, Jonathan; Pickup, Roger W.
In: Journal of Microbiological Methods, Vol. 33, No. 3, 01.08.1998, p. 221-226.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Porter, J & Pickup, RW 1998, 'Separation of natural populations of coliform bacteria from freshwater and sewage by magnetic-bead cell sorting', Journal of Microbiological Methods, vol. 33, no. 3, pp. 221-226. https://doi.org/10.1016/S0167-7012(98)00057-8

APA

Porter, J., & Pickup, R. W. (1998). Separation of natural populations of coliform bacteria from freshwater and sewage by magnetic-bead cell sorting. Journal of Microbiological Methods, 33(3), 221-226. https://doi.org/10.1016/S0167-7012(98)00057-8

Vancouver

Porter J, Pickup RW. Separation of natural populations of coliform bacteria from freshwater and sewage by magnetic-bead cell sorting. Journal of Microbiological Methods. 1998 Aug 1;33(3):221-226. doi: 10.1016/S0167-7012(98)00057-8

Author

Porter, Jonathan ; Pickup, Roger W. / Separation of natural populations of coliform bacteria from freshwater and sewage by magnetic-bead cell sorting. In: Journal of Microbiological Methods. 1998 ; Vol. 33, No. 3. pp. 221-226.

Bibtex

@article{989afbdbe904420fb465215a25d0b0bb,
title = "Separation of natural populations of coliform bacteria from freshwater and sewage by magnetic-bead cell sorting",
abstract = "Physical separation of natural populations of bacteria from a variety of environments via lectin-mediated magnetic bead separation was undertaken with the aim of obtaining a very clean population of target indigenous bacterial cells suitable for subsequent culture or molecular biological procedures. Recoveries varied with sample type, and were only effective from samples that contained a high culturable population on a general heterotroph agar (R2A) and on MacConkey agar. It was found that use of the lectin concanavalin A enabled selective recovery of coliform bacteria. Escherichia coli was separated with total coliforms, but was not preferentially selected. Separation using indirect magnetic capture was more effective and reliable than direct capture achieved using pre-labelled magnetic beads. The method may ultimately be of use in a monitoring procedure or targeted sampling programme. Copyright (C) 1998 Elsevier Science B.V.",
keywords = "Coliform bacteria, Concanavalin A, Magnetic beads, Separation",
author = "Jonathan Porter and Pickup, {Roger W.}",
year = "1998",
month = aug,
day = "1",
doi = "10.1016/S0167-7012(98)00057-8",
language = "English",
volume = "33",
pages = "221--226",
journal = "Journal of Microbiological Methods",
issn = "0167-7012",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - Separation of natural populations of coliform bacteria from freshwater and sewage by magnetic-bead cell sorting

AU - Porter, Jonathan

AU - Pickup, Roger W.

PY - 1998/8/1

Y1 - 1998/8/1

N2 - Physical separation of natural populations of bacteria from a variety of environments via lectin-mediated magnetic bead separation was undertaken with the aim of obtaining a very clean population of target indigenous bacterial cells suitable for subsequent culture or molecular biological procedures. Recoveries varied with sample type, and were only effective from samples that contained a high culturable population on a general heterotroph agar (R2A) and on MacConkey agar. It was found that use of the lectin concanavalin A enabled selective recovery of coliform bacteria. Escherichia coli was separated with total coliforms, but was not preferentially selected. Separation using indirect magnetic capture was more effective and reliable than direct capture achieved using pre-labelled magnetic beads. The method may ultimately be of use in a monitoring procedure or targeted sampling programme. Copyright (C) 1998 Elsevier Science B.V.

AB - Physical separation of natural populations of bacteria from a variety of environments via lectin-mediated magnetic bead separation was undertaken with the aim of obtaining a very clean population of target indigenous bacterial cells suitable for subsequent culture or molecular biological procedures. Recoveries varied with sample type, and were only effective from samples that contained a high culturable population on a general heterotroph agar (R2A) and on MacConkey agar. It was found that use of the lectin concanavalin A enabled selective recovery of coliform bacteria. Escherichia coli was separated with total coliforms, but was not preferentially selected. Separation using indirect magnetic capture was more effective and reliable than direct capture achieved using pre-labelled magnetic beads. The method may ultimately be of use in a monitoring procedure or targeted sampling programme. Copyright (C) 1998 Elsevier Science B.V.

KW - Coliform bacteria

KW - Concanavalin A

KW - Magnetic beads

KW - Separation

U2 - 10.1016/S0167-7012(98)00057-8

DO - 10.1016/S0167-7012(98)00057-8

M3 - Journal article

AN - SCOPUS:0032142982

VL - 33

SP - 221

EP - 226

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

SN - 0167-7012

IS - 3

ER -