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Sex pheromone activity in a single component of tergal gland extract of Lutzomyia longipalpis (Diptera Psychodidae) from Jacobina, Northeastern Brazil

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Sex pheromone activity in a single component of tergal gland extract of Lutzomyia longipalpis (Diptera Psychodidae) from Jacobina, Northeastern Brazil. / Hamilton, J. G.C.; Dougherty, M. J.; Ward, R. D.
In: Journal of Chemical Ecology, Vol. 20, No. 1, 01.1994, p. 141-151.

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@article{e895e60bf7b649b0b25bc57cf0081f1b,
title = "Sex pheromone activity in a single component of tergal gland extract of Lutzomyia longipalpis (Diptera Psychodidae) from Jacobina, Northeastern Brazil",
abstract = "The sex pheromone component of male Lutzomyia longipalpis tergal gland extract was isolated and its activity confirmed by bioassay. Whole tergal gland extract was analyzed by HPLC and fractions were collected as they eluted from the detector. Each fraction was tested in an attraction bioassay with virgin unfed female Lutzomyia longipalpis. HPLC analysis showed that whole extract contained several peaks; one large peak, one small peak and several minor peaks. Purity of the HPLC fractions was determined by GC analysis. The bioassays revealed that the large peak was responsible for most of the observed female behavior. The addition of the small peak to the large peak improved the response although by itself the small peak failed to elicit any significant behavior. Minor peaks failed to elicit any response. Chemical analysis revealed the large peak to be a relatively nonpolar hydrocarbon.",
keywords = "Diptera, gas chromatography, high-pressure liquid chromatography, Lutzomyia longipalpis, Psychodidae, sex pheromone, tergal gland",
author = "Hamilton, {J. G.C.} and Dougherty, {M. J.} and Ward, {R. D.}",
year = "1994",
month = jan,
doi = "10.1007/BF02065997",
language = "English",
volume = "20",
pages = "141--151",
journal = "Journal of Chemical Ecology",
issn = "0098-0331",
publisher = "Springer New York LLC",
number = "1",

}

RIS

TY - JOUR

T1 - Sex pheromone activity in a single component of tergal gland extract of Lutzomyia longipalpis (Diptera Psychodidae) from Jacobina, Northeastern Brazil

AU - Hamilton, J. G.C.

AU - Dougherty, M. J.

AU - Ward, R. D.

PY - 1994/1

Y1 - 1994/1

N2 - The sex pheromone component of male Lutzomyia longipalpis tergal gland extract was isolated and its activity confirmed by bioassay. Whole tergal gland extract was analyzed by HPLC and fractions were collected as they eluted from the detector. Each fraction was tested in an attraction bioassay with virgin unfed female Lutzomyia longipalpis. HPLC analysis showed that whole extract contained several peaks; one large peak, one small peak and several minor peaks. Purity of the HPLC fractions was determined by GC analysis. The bioassays revealed that the large peak was responsible for most of the observed female behavior. The addition of the small peak to the large peak improved the response although by itself the small peak failed to elicit any significant behavior. Minor peaks failed to elicit any response. Chemical analysis revealed the large peak to be a relatively nonpolar hydrocarbon.

AB - The sex pheromone component of male Lutzomyia longipalpis tergal gland extract was isolated and its activity confirmed by bioassay. Whole tergal gland extract was analyzed by HPLC and fractions were collected as they eluted from the detector. Each fraction was tested in an attraction bioassay with virgin unfed female Lutzomyia longipalpis. HPLC analysis showed that whole extract contained several peaks; one large peak, one small peak and several minor peaks. Purity of the HPLC fractions was determined by GC analysis. The bioassays revealed that the large peak was responsible for most of the observed female behavior. The addition of the small peak to the large peak improved the response although by itself the small peak failed to elicit any significant behavior. Minor peaks failed to elicit any response. Chemical analysis revealed the large peak to be a relatively nonpolar hydrocarbon.

KW - Diptera

KW - gas chromatography

KW - high-pressure liquid chromatography

KW - Lutzomyia longipalpis

KW - Psychodidae

KW - sex pheromone

KW - tergal gland

U2 - 10.1007/BF02065997

DO - 10.1007/BF02065997

M3 - Journal article

AN - SCOPUS:0028011518

VL - 20

SP - 141

EP - 151

JO - Journal of Chemical Ecology

JF - Journal of Chemical Ecology

SN - 0098-0331

IS - 1

ER -