Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Sex pheromone activity in a single component of tergal gland extract of Lutzomyia longipalpis (Diptera Psychodidae) from Jacobina, Northeastern Brazil
AU - Hamilton, J. G.C.
AU - Dougherty, M. J.
AU - Ward, R. D.
PY - 1994/1
Y1 - 1994/1
N2 - The sex pheromone component of male Lutzomyia longipalpis tergal gland extract was isolated and its activity confirmed by bioassay. Whole tergal gland extract was analyzed by HPLC and fractions were collected as they eluted from the detector. Each fraction was tested in an attraction bioassay with virgin unfed female Lutzomyia longipalpis. HPLC analysis showed that whole extract contained several peaks; one large peak, one small peak and several minor peaks. Purity of the HPLC fractions was determined by GC analysis. The bioassays revealed that the large peak was responsible for most of the observed female behavior. The addition of the small peak to the large peak improved the response although by itself the small peak failed to elicit any significant behavior. Minor peaks failed to elicit any response. Chemical analysis revealed the large peak to be a relatively nonpolar hydrocarbon.
AB - The sex pheromone component of male Lutzomyia longipalpis tergal gland extract was isolated and its activity confirmed by bioassay. Whole tergal gland extract was analyzed by HPLC and fractions were collected as they eluted from the detector. Each fraction was tested in an attraction bioassay with virgin unfed female Lutzomyia longipalpis. HPLC analysis showed that whole extract contained several peaks; one large peak, one small peak and several minor peaks. Purity of the HPLC fractions was determined by GC analysis. The bioassays revealed that the large peak was responsible for most of the observed female behavior. The addition of the small peak to the large peak improved the response although by itself the small peak failed to elicit any significant behavior. Minor peaks failed to elicit any response. Chemical analysis revealed the large peak to be a relatively nonpolar hydrocarbon.
KW - Diptera
KW - gas chromatography
KW - high-pressure liquid chromatography
KW - Lutzomyia longipalpis
KW - Psychodidae
KW - sex pheromone
KW - tergal gland
U2 - 10.1007/BF02065997
DO - 10.1007/BF02065997
M3 - Journal article
AN - SCOPUS:0028011518
VL - 20
SP - 141
EP - 151
JO - Journal of Chemical Ecology
JF - Journal of Chemical Ecology
SN - 0098-0331
IS - 1
ER -