Rights statement: This is the peer reviewed version of the following article: Chenge‐Espinosa, M. , Cordoba, E. , Romero‐Guido, C. , Toledo‐Ortiz, G. and León, P. (2018), Shedding light on the methylerythritol phosphate (MEP)‐pathway: long hypocotyl 5 (HY5)/phytochrome‐interacting factors (PIFs) transcription factors modulating key limiting steps. Plant J, 96: 828-841. doi:10.1111/tpj.14071 which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/tpj.14071 This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
Accepted author manuscript, 780 KB, PDF document
Available under license: CC BY-NC: Creative Commons Attribution-NonCommercial 4.0 International License
Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Shedding light on the methylerythritol phosphate (MEP)-pathway
T2 - long hypocotyl 5 (HY5)/phytochrome-interacting factors (PIFs) transcription factors modulating key limiting steps
AU - Chenge-Espinosa, M.
AU - Cordoba, E.
AU - Romero-Guido, C.
AU - Toledo-Ortiz, G.
AU - León, P.
N1 - This is the peer reviewed version of the following article: Chenge‐Espinosa, M. , Cordoba, E. , Romero‐Guido, C. , Toledo‐Ortiz, G. and León, P. (2018), Shedding light on the methylerythritol phosphate (MEP)‐pathway: long hypocotyl 5 (HY5)/phytochrome‐interacting factors (PIFs) transcription factors modulating key limiting steps. Plant J, 96: 828-841. doi:10.1111/tpj.14071 which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/tpj.14071 This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
PY - 2018/11
Y1 - 2018/11
N2 - The plastidial methylerythritol phosphate (MEP) pathway is an essential route for plants as the source of precursors for all plastidial isoprenoids, many of which are of medical and biotechnological importance. The MEP pathway is highly sensitive to environmental cues as many of these compounds are linked to photosynthesis and growth and light is one of the main regulatory factors. However, the mechanisms coordinating the MEP pathway with light cues are not fully understood. Here we demonstrate that by a differential direct transcriptional modulation, via the key-master integrators of light signal transduction HY5 and PIFs which target the genes that encode the rate-controlling DXS1, DXR and HDR enzymes, light imposes a direct, rapid and potentially multi-faceted response that leads to unique protein dynamics of this pathway, resulting in a significant difference in the protein levels. For DXS1, PIF1/HY5 act as a direct activation/suppression module. In contrast, DXR accumulation in response to light results from HY5 induction with minor contribution of de-repression by PIF1. Finally, HDR transcription increases in the light exclusively by suppression of the PIFs repression. This is an example of how light signaling components can differentially multi-target the initial steps of a pathway whose products branch downstream to all chloroplastic isoprenoids. These findings demonstrate the diversity and flexibility of light signaling components that optimize key biochemical pathways essential for plant growth. © 2018 The Authors The Plant Journal © 2018 John Wiley & Sons Ltd
AB - The plastidial methylerythritol phosphate (MEP) pathway is an essential route for plants as the source of precursors for all plastidial isoprenoids, many of which are of medical and biotechnological importance. The MEP pathway is highly sensitive to environmental cues as many of these compounds are linked to photosynthesis and growth and light is one of the main regulatory factors. However, the mechanisms coordinating the MEP pathway with light cues are not fully understood. Here we demonstrate that by a differential direct transcriptional modulation, via the key-master integrators of light signal transduction HY5 and PIFs which target the genes that encode the rate-controlling DXS1, DXR and HDR enzymes, light imposes a direct, rapid and potentially multi-faceted response that leads to unique protein dynamics of this pathway, resulting in a significant difference in the protein levels. For DXS1, PIF1/HY5 act as a direct activation/suppression module. In contrast, DXR accumulation in response to light results from HY5 induction with minor contribution of de-repression by PIF1. Finally, HDR transcription increases in the light exclusively by suppression of the PIFs repression. This is an example of how light signaling components can differentially multi-target the initial steps of a pathway whose products branch downstream to all chloroplastic isoprenoids. These findings demonstrate the diversity and flexibility of light signaling components that optimize key biochemical pathways essential for plant growth. © 2018 The Authors The Plant Journal © 2018 John Wiley & Sons Ltd
KW - Arabidopsis thaliana
KW - DXR and HDR enzymes
KW - DXS1
KW - isoprenoids
KW - light responses
KW - long hypocotyl 5
KW - MEP pathway
KW - phytochrome-interacting factors
KW - rate-limiting enzymes
KW - Enzymes
KW - Lipids
KW - Signal transduction
KW - Isoprenoids
KW - Light response
KW - MEP pathways
KW - Phytochrome-interacting factors
KW - Rate-limiting enzymes
KW - Transcription
U2 - 10.1111/tpj.14071
DO - 10.1111/tpj.14071
M3 - Journal article
VL - 96
SP - 828
EP - 841
JO - Plant Journal
JF - Plant Journal
SN - 0960-7412
IS - 4
ER -