Rights statement: This is the author’s version of a work that was accepted for publication in Talanta. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Talanta, 228, 2021 DOI: 10.1016/j.talanta.2021.122211
Accepted author manuscript, 2.47 MB, PDF document
Available under license: CC BY-NC-ND
Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Single virus inductively coupled plasma mass spectroscopy analysis
T2 - A comprehensive study
AU - Degueldre, C.
N1 - This is the author’s version of a work that was accepted for publication in Talanta. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Talanta, 228, 2021 DOI: 10.1016/j.talanta.2021.122211
PY - 2021/6/1
Y1 - 2021/6/1
N2 - The characterisation of individual nanoparticles by single particle ICP-MS (SP-ICP-MS) has paved the way for the analysis of smallest biological systems. This study suggests to adapting this method for single viruses (SV) identification and counting. With high resolution multi-channel sector field (MC SF) ICP-MS records in SV detection mode, the counting of master and key ions can allow analysis and identification of single viruses. The counting of 2–500 virial units can be performed in 20 s. Analyses are proposed to be carried out in Ar torch for master ions: 12C+, 13C+, 14N+, 15N+, and key ions 31P+, 32S+, 33S+ and 34S+. All interferences are discussed in detail. The use of high resolution SF ICP-MS is recommended while options with anaerobic/aerobic atmospheres are explored to upgrade the analysis when using quadrupole ICP-MS. Application for two virus types (SARS-COV2 and bacteriophage T5) is investigated using time scan and fixed mass analysis for the selected virus ions allowing characterisation of the species using the N/C, P/C and S/C molar ratio's and quantification of their number concentration.
AB - The characterisation of individual nanoparticles by single particle ICP-MS (SP-ICP-MS) has paved the way for the analysis of smallest biological systems. This study suggests to adapting this method for single viruses (SV) identification and counting. With high resolution multi-channel sector field (MC SF) ICP-MS records in SV detection mode, the counting of master and key ions can allow analysis and identification of single viruses. The counting of 2–500 virial units can be performed in 20 s. Analyses are proposed to be carried out in Ar torch for master ions: 12C+, 13C+, 14N+, 15N+, and key ions 31P+, 32S+, 33S+ and 34S+. All interferences are discussed in detail. The use of high resolution SF ICP-MS is recommended while options with anaerobic/aerobic atmospheres are explored to upgrade the analysis when using quadrupole ICP-MS. Application for two virus types (SARS-COV2 and bacteriophage T5) is investigated using time scan and fixed mass analysis for the selected virus ions allowing characterisation of the species using the N/C, P/C and S/C molar ratio's and quantification of their number concentration.
KW - Individual virus analysis
KW - single virus
KW - SP ICP-MS
KW - Virus counting
KW - Virus identification
KW - Diseases
KW - Inductively coupled plasma
KW - Inductively coupled plasma mass spectrometry
KW - Ions
KW - Mass spectrometers
KW - Molar concentration
KW - Molar ratio
KW - Anaerobic/aerobic
KW - Detection mode
KW - High resolution
KW - Individual nanoparticles
KW - Inductively coupled plasma mass spectroscopies
KW - Number concentration
KW - Quadrupole icp-ms
KW - Single particle
KW - Viruses
U2 - 10.1016/j.talanta.2021.122211
DO - 10.1016/j.talanta.2021.122211
M3 - Journal article
VL - 228
JO - Talanta
JF - Talanta
SN - 0039-9140
M1 - 122211
ER -