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    Rights statement: This is the author’s version of a work that was accepted for publication in Talanta. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Talanta, 228, 2021 DOI: 10.1016/j.talanta.2021.122211

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Single virus inductively coupled plasma mass spectroscopy analysis: A comprehensive study

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Single virus inductively coupled plasma mass spectroscopy analysis : A comprehensive study. / Degueldre, C.

In: Talanta, Vol. 228, 122211, 01.06.2021.

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Degueldre C. Single virus inductively coupled plasma mass spectroscopy analysis: A comprehensive study. Talanta. 2021 Jun 1;228:122211. Epub 2021 Feb 11. doi: 10.1016/j.talanta.2021.122211

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@article{ff7c4cd8e633435e9d83a2115e751c99,
title = "Single virus inductively coupled plasma mass spectroscopy analysis: A comprehensive study",
abstract = "The characterisation of individual nanoparticles by single particle ICP-MS (SP-ICP-MS) has paved the way for the analysis of smallest biological systems. This study suggests to adapting this method for single viruses (SV) identification and counting. With high resolution multi-channel sector field (MC SF) ICP-MS records in SV detection mode, the counting of master and key ions can allow analysis and identification of single viruses. The counting of 2–500 virial units can be performed in 20 s. Analyses are proposed to be carried out in Ar torch for master ions: 12C+, 13C+, 14N+, 15N+, and key ions 31P+, 32S+, 33S+ and 34S+. All interferences are discussed in detail. The use of high resolution SF ICP-MS is recommended while options with anaerobic/aerobic atmospheres are explored to upgrade the analysis when using quadrupole ICP-MS. Application for two virus types (SARS-COV2 and bacteriophage T5) is investigated using time scan and fixed mass analysis for the selected virus ions allowing characterisation of the species using the N/C, P/C and S/C molar ratio's and quantification of their number concentration. ",
keywords = "Individual virus analysis, single virus, SP ICP-MS, Virus counting, Virus identification, Diseases, Inductively coupled plasma, Inductively coupled plasma mass spectrometry, Ions, Mass spectrometers, Molar concentration, Molar ratio, Anaerobic/aerobic, Detection mode, High resolution, Individual nanoparticles, Inductively coupled plasma mass spectroscopies, Number concentration, Quadrupole icp-ms, Single particle, Viruses",
author = "C. Degueldre",
note = "This is the author{\textquoteright}s version of a work that was accepted for publication in Talanta. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Talanta, 228, 2021 DOI: 10.1016/j.talanta.2021.122211",
year = "2021",
month = jun,
day = "1",
doi = "10.1016/j.talanta.2021.122211",
language = "English",
volume = "228",
journal = "Talanta",
issn = "0039-9140",
publisher = "Elsevier Science B.V.",

}

RIS

TY - JOUR

T1 - Single virus inductively coupled plasma mass spectroscopy analysis

T2 - A comprehensive study

AU - Degueldre, C.

N1 - This is the author’s version of a work that was accepted for publication in Talanta. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Talanta, 228, 2021 DOI: 10.1016/j.talanta.2021.122211

PY - 2021/6/1

Y1 - 2021/6/1

N2 - The characterisation of individual nanoparticles by single particle ICP-MS (SP-ICP-MS) has paved the way for the analysis of smallest biological systems. This study suggests to adapting this method for single viruses (SV) identification and counting. With high resolution multi-channel sector field (MC SF) ICP-MS records in SV detection mode, the counting of master and key ions can allow analysis and identification of single viruses. The counting of 2–500 virial units can be performed in 20 s. Analyses are proposed to be carried out in Ar torch for master ions: 12C+, 13C+, 14N+, 15N+, and key ions 31P+, 32S+, 33S+ and 34S+. All interferences are discussed in detail. The use of high resolution SF ICP-MS is recommended while options with anaerobic/aerobic atmospheres are explored to upgrade the analysis when using quadrupole ICP-MS. Application for two virus types (SARS-COV2 and bacteriophage T5) is investigated using time scan and fixed mass analysis for the selected virus ions allowing characterisation of the species using the N/C, P/C and S/C molar ratio's and quantification of their number concentration.

AB - The characterisation of individual nanoparticles by single particle ICP-MS (SP-ICP-MS) has paved the way for the analysis of smallest biological systems. This study suggests to adapting this method for single viruses (SV) identification and counting. With high resolution multi-channel sector field (MC SF) ICP-MS records in SV detection mode, the counting of master and key ions can allow analysis and identification of single viruses. The counting of 2–500 virial units can be performed in 20 s. Analyses are proposed to be carried out in Ar torch for master ions: 12C+, 13C+, 14N+, 15N+, and key ions 31P+, 32S+, 33S+ and 34S+. All interferences are discussed in detail. The use of high resolution SF ICP-MS is recommended while options with anaerobic/aerobic atmospheres are explored to upgrade the analysis when using quadrupole ICP-MS. Application for two virus types (SARS-COV2 and bacteriophage T5) is investigated using time scan and fixed mass analysis for the selected virus ions allowing characterisation of the species using the N/C, P/C and S/C molar ratio's and quantification of their number concentration.

KW - Individual virus analysis

KW - single virus

KW - SP ICP-MS

KW - Virus counting

KW - Virus identification

KW - Diseases

KW - Inductively coupled plasma

KW - Inductively coupled plasma mass spectrometry

KW - Ions

KW - Mass spectrometers

KW - Molar concentration

KW - Molar ratio

KW - Anaerobic/aerobic

KW - Detection mode

KW - High resolution

KW - Individual nanoparticles

KW - Inductively coupled plasma mass spectroscopies

KW - Number concentration

KW - Quadrupole icp-ms

KW - Single particle

KW - Viruses

U2 - 10.1016/j.talanta.2021.122211

DO - 10.1016/j.talanta.2021.122211

M3 - Journal article

VL - 228

JO - Talanta

JF - Talanta

SN - 0039-9140

M1 - 122211

ER -