Research output: Exhibits, objects and web-based outputs › Digital or Visual Products
Research output: Exhibits, objects and web-based outputs › Digital or Visual Products
}
TY - ADVS
T1 - Spin Saturation Transfer Difference NMR (SSTD NMR): a new tool to obtain kinetic parameters of chemical exchange processes
AU - Quirós, María Teresa
AU - Macdonald, Colin
AU - Angulo, Jesús
AU - Paz, María
PY - 2016/11/12
Y1 - 2016/11/12
N2 - This detailed protocol describes the new Spin Saturation Transfer Difference Nuclear Magnetic Resonance protocol (SSTD NMR), recently developed in our group to study processes of mutual-site chemical exchange that are difficult to analyze by traditional methods. As the name suggests, this method combines the Spin Saturation Transfer method used for small molecules, with the Saturation Transfer Difference (STD) NMR method employed for the study of protein-ligand interactions, by measuring transient spin saturation transfer along increasing saturation times (build-up curves) in small organic and organometallic molecules undergoing chemical exchange.
AB - This detailed protocol describes the new Spin Saturation Transfer Difference Nuclear Magnetic Resonance protocol (SSTD NMR), recently developed in our group to study processes of mutual-site chemical exchange that are difficult to analyze by traditional methods. As the name suggests, this method combines the Spin Saturation Transfer method used for small molecules, with the Saturation Transfer Difference (STD) NMR method employed for the study of protein-ligand interactions, by measuring transient spin saturation transfer along increasing saturation times (build-up curves) in small organic and organometallic molecules undergoing chemical exchange.
KW - NMMR
KW - spin saturation transfer
KW - saturation transfer difference
KW - kinetics
KW - chemical exchange
KW - NOE
KW - thermodynamic parameters
KW - SSTD NMR
U2 - 10.3791/54499
DO - 10.3791/54499
M3 - Digital or Visual Products
ER -