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Stable Isotope Probing of an Algal Bloom To Identify Uncultivated Members of the Rhodobacteraceae Associated with Low-Molecular-Weight Polycyclic Aromatic Hydrocarbon Degradation

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Stable Isotope Probing of an Algal Bloom To Identify Uncultivated Members of the Rhodobacteraceae Associated with Low-Molecular-Weight Polycyclic Aromatic Hydrocarbon Degradation. / Gutierrez, Tony; Singleton, David R.; Aitken, Michael D. et al.

In: Applied and Environmental Microbiology, Vol. 77, No. 21, 11.2011, p. 7856-7860.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Gutierrez, T, Singleton, DR, Aitken, MD & Semple, KT 2011, 'Stable Isotope Probing of an Algal Bloom To Identify Uncultivated Members of the Rhodobacteraceae Associated with Low-Molecular-Weight Polycyclic Aromatic Hydrocarbon Degradation', Applied and Environmental Microbiology, vol. 77, no. 21, pp. 7856-7860. https://doi.org/10.1128/AEM.06200-11

APA

Gutierrez, T., Singleton, D. R., Aitken, M. D., & Semple, K. T. (2011). Stable Isotope Probing of an Algal Bloom To Identify Uncultivated Members of the Rhodobacteraceae Associated with Low-Molecular-Weight Polycyclic Aromatic Hydrocarbon Degradation. Applied and Environmental Microbiology, 77(21), 7856-7860. https://doi.org/10.1128/AEM.06200-11

Vancouver

Gutierrez T, Singleton DR, Aitken MD, Semple KT. Stable Isotope Probing of an Algal Bloom To Identify Uncultivated Members of the Rhodobacteraceae Associated with Low-Molecular-Weight Polycyclic Aromatic Hydrocarbon Degradation. Applied and Environmental Microbiology. 2011 Nov;77(21):7856-7860. doi: 10.1128/AEM.06200-11

Author

Gutierrez, Tony ; Singleton, David R. ; Aitken, Michael D. et al. / Stable Isotope Probing of an Algal Bloom To Identify Uncultivated Members of the Rhodobacteraceae Associated with Low-Molecular-Weight Polycyclic Aromatic Hydrocarbon Degradation. In: Applied and Environmental Microbiology. 2011 ; Vol. 77, No. 21. pp. 7856-7860.

Bibtex

@article{b42d16c4e1674e928ade741661d5fdb3,
title = "Stable Isotope Probing of an Algal Bloom To Identify Uncultivated Members of the Rhodobacteraceae Associated with Low-Molecular-Weight Polycyclic Aromatic Hydrocarbon Degradation",
abstract = "Polycyclic aromatic hydrocarbon (PAH)-degrading bacteria associated with an algal bloom in Tampa Bay, FL, were investigated by stable isotope probing (SIP) with uniformly labeled [C-13]naphthalene. The dominant sequences in clone libraries constructed from C-13-enriched bacterial DNA (from naphthalene enrichments) were identified as uncharacterized members of the family Rhodobacteraceae. Quantitative PCR primers targeting the 16S rRNA gene of these uncultivated organisms were used to determine their abundance in incubations amended with unlabeled naphthalene and phenanthrene, both of which showed substantial increases in gene copy numbers during the experiments. As demonstrated by this work, the application of uniformly C-13-labeled PAHs in SIP experiments can successfully be used to identify novel PAH-degrading bacteria in marine waters.",
author = "Tony Gutierrez and Singleton, {David R.} and Aitken, {Michael D.} and Semple, {Kirk T.}",
year = "2011",
month = nov,
doi = "10.1128/AEM.06200-11",
language = "English",
volume = "77",
pages = "7856--7860",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "21",

}

RIS

TY - JOUR

T1 - Stable Isotope Probing of an Algal Bloom To Identify Uncultivated Members of the Rhodobacteraceae Associated with Low-Molecular-Weight Polycyclic Aromatic Hydrocarbon Degradation

AU - Gutierrez, Tony

AU - Singleton, David R.

AU - Aitken, Michael D.

AU - Semple, Kirk T.

PY - 2011/11

Y1 - 2011/11

N2 - Polycyclic aromatic hydrocarbon (PAH)-degrading bacteria associated with an algal bloom in Tampa Bay, FL, were investigated by stable isotope probing (SIP) with uniformly labeled [C-13]naphthalene. The dominant sequences in clone libraries constructed from C-13-enriched bacterial DNA (from naphthalene enrichments) were identified as uncharacterized members of the family Rhodobacteraceae. Quantitative PCR primers targeting the 16S rRNA gene of these uncultivated organisms were used to determine their abundance in incubations amended with unlabeled naphthalene and phenanthrene, both of which showed substantial increases in gene copy numbers during the experiments. As demonstrated by this work, the application of uniformly C-13-labeled PAHs in SIP experiments can successfully be used to identify novel PAH-degrading bacteria in marine waters.

AB - Polycyclic aromatic hydrocarbon (PAH)-degrading bacteria associated with an algal bloom in Tampa Bay, FL, were investigated by stable isotope probing (SIP) with uniformly labeled [C-13]naphthalene. The dominant sequences in clone libraries constructed from C-13-enriched bacterial DNA (from naphthalene enrichments) were identified as uncharacterized members of the family Rhodobacteraceae. Quantitative PCR primers targeting the 16S rRNA gene of these uncultivated organisms were used to determine their abundance in incubations amended with unlabeled naphthalene and phenanthrene, both of which showed substantial increases in gene copy numbers during the experiments. As demonstrated by this work, the application of uniformly C-13-labeled PAHs in SIP experiments can successfully be used to identify novel PAH-degrading bacteria in marine waters.

UR - http://www.scopus.com/inward/record.url?scp=83155184860&partnerID=8YFLogxK

U2 - 10.1128/AEM.06200-11

DO - 10.1128/AEM.06200-11

M3 - Journal article

VL - 77

SP - 7856

EP - 7860

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 21

ER -