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Sterilized, freeze-dried amniotic membrane : a useful substrate for ocular surface reconstruction.

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Sterilized, freeze-dried amniotic membrane : a useful substrate for ocular surface reconstruction. / Nakamura, Takahiro; Yoshitani, Makoto; Rigby, Helen et al.
In: Investigative Ophthalmology and Visual Science, Vol. 45, No. 1, 01.2004, p. 93-99.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Nakamura, T, Yoshitani, M, Rigby, H, Fullwood, NJ, Ito, W, Inatomi, T, Sotozono, C, Nakamura, T, Shimizu, Y & Kinoshita, S 2004, 'Sterilized, freeze-dried amniotic membrane : a useful substrate for ocular surface reconstruction.', Investigative Ophthalmology and Visual Science, vol. 45, no. 1, pp. 93-99. https://doi.org/10.1167/iovs.03-0752

APA

Nakamura, T., Yoshitani, M., Rigby, H., Fullwood, N. J., Ito, W., Inatomi, T., Sotozono, C., Nakamura, T., Shimizu, Y., & Kinoshita, S. (2004). Sterilized, freeze-dried amniotic membrane : a useful substrate for ocular surface reconstruction. Investigative Ophthalmology and Visual Science, 45(1), 93-99. https://doi.org/10.1167/iovs.03-0752

Vancouver

Nakamura T, Yoshitani M, Rigby H, Fullwood NJ, Ito W, Inatomi T et al. Sterilized, freeze-dried amniotic membrane : a useful substrate for ocular surface reconstruction. Investigative Ophthalmology and Visual Science. 2004 Jan;45(1):93-99. doi: 10.1167/iovs.03-0752

Author

Nakamura, Takahiro ; Yoshitani, Makoto ; Rigby, Helen et al. / Sterilized, freeze-dried amniotic membrane : a useful substrate for ocular surface reconstruction. In: Investigative Ophthalmology and Visual Science. 2004 ; Vol. 45, No. 1. pp. 93-99.

Bibtex

@article{b2c07f679389432596fffeb182b188a0,
title = "Sterilized, freeze-dried amniotic membrane : a useful substrate for ocular surface reconstruction.",
abstract = "PURPOSE. To examine the feasibility of using sterilized, freeze-dried amniotic membrane (FD-AM) as a substrate for cultivating autologous corneal epithelial cells for ocular surface reconstruction. METHODS. Human AM deprived of amniotic epithelial cells by incubation with EDTA was freeze dried, vacuum packed, and sterilized with -irradiation. The resultant FD-AM was characterized for its physical, biological, and morphologic properties by stretch stress tests, immunohistochemistry, electron microscopy, and cell culture. In addition, 3 weeks after an ocular surface injury, the conjunctivalized corneal surfaces of eyes in eight rabbits were surgically reconstructed by transplantation of autologous cultivated corneal epithelial cells on FD-AM. RESULTS. A stretch stress test revealed no significant differences between sterilized FD-AM and cryopreserved AM. Immunohistochemistry for several extracellular matrix molecules and electron microscopic analysis of FD-AM revealed that the process of drying and irradiation did not affect its biological and morphologic properties. The corneal epithelial cells cultivated on FD-AM had four to five stratified, well-differentiated cell layers. Corneas that were grafted with the cultivated corneal epithelial cells on FD-AM were clear and were all epithelialized at 10 days after surgery. CONCLUSIONS. The sterilized, freeze-dried AM retained most of the physical, biological, and morphologic characteristics of cryopreserved AM; consequently, it is a useful biomaterial for ocular surface reconstruction.",
author = "Takahiro Nakamura and Makoto Yoshitani and Helen Rigby and Fullwood, {Nigel J.} and Wakana Ito and Tsutomu Inatomi and Chie Sotozono and Tatsuo Nakamura and Yasuhiko Shimizu and Shigeru Kinoshita",
year = "2004",
month = jan,
doi = "10.1167/iovs.03-0752",
language = "English",
volume = "45",
pages = "93--99",
journal = "Investigative Ophthalmology and Visual Science",
issn = "1552-5783",
publisher = "ASSOC RESEARCH VISION OPHTHALMOLOGY INC",
number = "1",

}

RIS

TY - JOUR

T1 - Sterilized, freeze-dried amniotic membrane : a useful substrate for ocular surface reconstruction.

AU - Nakamura, Takahiro

AU - Yoshitani, Makoto

AU - Rigby, Helen

AU - Fullwood, Nigel J.

AU - Ito, Wakana

AU - Inatomi, Tsutomu

AU - Sotozono, Chie

AU - Nakamura, Tatsuo

AU - Shimizu, Yasuhiko

AU - Kinoshita, Shigeru

PY - 2004/1

Y1 - 2004/1

N2 - PURPOSE. To examine the feasibility of using sterilized, freeze-dried amniotic membrane (FD-AM) as a substrate for cultivating autologous corneal epithelial cells for ocular surface reconstruction. METHODS. Human AM deprived of amniotic epithelial cells by incubation with EDTA was freeze dried, vacuum packed, and sterilized with -irradiation. The resultant FD-AM was characterized for its physical, biological, and morphologic properties by stretch stress tests, immunohistochemistry, electron microscopy, and cell culture. In addition, 3 weeks after an ocular surface injury, the conjunctivalized corneal surfaces of eyes in eight rabbits were surgically reconstructed by transplantation of autologous cultivated corneal epithelial cells on FD-AM. RESULTS. A stretch stress test revealed no significant differences between sterilized FD-AM and cryopreserved AM. Immunohistochemistry for several extracellular matrix molecules and electron microscopic analysis of FD-AM revealed that the process of drying and irradiation did not affect its biological and morphologic properties. The corneal epithelial cells cultivated on FD-AM had four to five stratified, well-differentiated cell layers. Corneas that were grafted with the cultivated corneal epithelial cells on FD-AM were clear and were all epithelialized at 10 days after surgery. CONCLUSIONS. The sterilized, freeze-dried AM retained most of the physical, biological, and morphologic characteristics of cryopreserved AM; consequently, it is a useful biomaterial for ocular surface reconstruction.

AB - PURPOSE. To examine the feasibility of using sterilized, freeze-dried amniotic membrane (FD-AM) as a substrate for cultivating autologous corneal epithelial cells for ocular surface reconstruction. METHODS. Human AM deprived of amniotic epithelial cells by incubation with EDTA was freeze dried, vacuum packed, and sterilized with -irradiation. The resultant FD-AM was characterized for its physical, biological, and morphologic properties by stretch stress tests, immunohistochemistry, electron microscopy, and cell culture. In addition, 3 weeks after an ocular surface injury, the conjunctivalized corneal surfaces of eyes in eight rabbits were surgically reconstructed by transplantation of autologous cultivated corneal epithelial cells on FD-AM. RESULTS. A stretch stress test revealed no significant differences between sterilized FD-AM and cryopreserved AM. Immunohistochemistry for several extracellular matrix molecules and electron microscopic analysis of FD-AM revealed that the process of drying and irradiation did not affect its biological and morphologic properties. The corneal epithelial cells cultivated on FD-AM had four to five stratified, well-differentiated cell layers. Corneas that were grafted with the cultivated corneal epithelial cells on FD-AM were clear and were all epithelialized at 10 days after surgery. CONCLUSIONS. The sterilized, freeze-dried AM retained most of the physical, biological, and morphologic characteristics of cryopreserved AM; consequently, it is a useful biomaterial for ocular surface reconstruction.

U2 - 10.1167/iovs.03-0752

DO - 10.1167/iovs.03-0752

M3 - Journal article

VL - 45

SP - 93

EP - 99

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 1552-5783

IS - 1

ER -