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Stomatal Function Requires Pectin De-methyl-esterification of the Guard Cell Wall

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Stomatal Function Requires Pectin De-methyl-esterification of the Guard Cell Wall. / Amsbury, Sam; Hunt, Lee; Elhaddad, Nagat; Baillie, Alice; Lundgren, Marjorie; Verhertbruggen, Yves; Scheller, Henrik V.; Knox, J. Paul; Fleming, Andrew J.; Gray, Julie E.

In: Current Biology, Vol. 26, No. 21, 07.11.2016, p. 2899-2906.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Amsbury, S, Hunt, L, Elhaddad, N, Baillie, A, Lundgren, M, Verhertbruggen, Y, Scheller, HV, Knox, JP, Fleming, AJ & Gray, JE 2016, 'Stomatal Function Requires Pectin De-methyl-esterification of the Guard Cell Wall', Current Biology, vol. 26, no. 21, pp. 2899-2906. https://doi.org/10.1016/j.cub.2016.08.021

APA

Amsbury, S., Hunt, L., Elhaddad, N., Baillie, A., Lundgren, M., Verhertbruggen, Y., Scheller, H. V., Knox, J. P., Fleming, A. J., & Gray, J. E. (2016). Stomatal Function Requires Pectin De-methyl-esterification of the Guard Cell Wall. Current Biology, 26(21), 2899-2906. https://doi.org/10.1016/j.cub.2016.08.021

Vancouver

Amsbury S, Hunt L, Elhaddad N, Baillie A, Lundgren M, Verhertbruggen Y et al. Stomatal Function Requires Pectin De-methyl-esterification of the Guard Cell Wall. Current Biology. 2016 Nov 7;26(21):2899-2906. https://doi.org/10.1016/j.cub.2016.08.021

Author

Amsbury, Sam ; Hunt, Lee ; Elhaddad, Nagat ; Baillie, Alice ; Lundgren, Marjorie ; Verhertbruggen, Yves ; Scheller, Henrik V. ; Knox, J. Paul ; Fleming, Andrew J. ; Gray, Julie E. / Stomatal Function Requires Pectin De-methyl-esterification of the Guard Cell Wall. In: Current Biology. 2016 ; Vol. 26, No. 21. pp. 2899-2906.

Bibtex

@article{f3911dfe787d440992b66928c523589a,
title = "Stomatal Function Requires Pectin De-methyl-esterification of the Guard Cell Wall",
abstract = "Stomatal opening and closure depends on changes in turgor pressure acting within guard cells to alter cell shape [1]. The extent of these shape changes is limited by the mechanical properties of the cells, which will be largely dependent on the structure of the cell walls. Although it has long been observed that guard cells are anisotropic due to differential thickening and the orientation of cellulose microfibrils [2], our understanding of the composition of the cell wall that allows them to undergo repeated swelling and deflation remains surprisingly poor. Here, we show that the walls of guard cells are rich in un-esterified pectins. We identify a pectin methylesterase gene, PME6, which is highly expressed in guard cells and required for stomatal function. pme6-1 mutant guard cells have walls enriched in methyl-esterified pectin and show a decreased dynamic range in response to triggers of stomatal opening/closure, including elevated osmoticum, suggesting that abrogation of stomatal function reflects a mechanical change in the guard cell wall. Altered stomatal function leads to increased conductance and evaporative cooling, as well as decreased plant growth. The growth defect of the pme6-1 mutant is rescued by maintaining the plants in elevated CO2, substantiating gas exchange analyses, indicating that the mutant stomata can bestow an improved assimilation rate. Restoration of PME6 rescues guard cell wall pectin methyl-esterification status, stomatal function, and plant growth. Our results establish a link between gene expression in guard cells and their cell wall properties, with a corresponding effect on stomatal function and plant physiology.",
keywords = "Arabidopsis, cell wall, guard cell, pectin, stomata",
author = "Sam Amsbury and Lee Hunt and Nagat Elhaddad and Alice Baillie and Marjorie Lundgren and Yves Verhertbruggen and Scheller, {Henrik V.} and Knox, {J. Paul} and Fleming, {Andrew J.} and Gray, {Julie E.}",
year = "2016",
month = nov,
day = "7",
doi = "10.1016/j.cub.2016.08.021",
language = "English",
volume = "26",
pages = "2899--2906",
journal = "Current biology : CB",
issn = "0960-9822",
publisher = "CELL PRESS",
number = "21",

}

RIS

TY - JOUR

T1 - Stomatal Function Requires Pectin De-methyl-esterification of the Guard Cell Wall

AU - Amsbury, Sam

AU - Hunt, Lee

AU - Elhaddad, Nagat

AU - Baillie, Alice

AU - Lundgren, Marjorie

AU - Verhertbruggen, Yves

AU - Scheller, Henrik V.

AU - Knox, J. Paul

AU - Fleming, Andrew J.

AU - Gray, Julie E.

PY - 2016/11/7

Y1 - 2016/11/7

N2 - Stomatal opening and closure depends on changes in turgor pressure acting within guard cells to alter cell shape [1]. The extent of these shape changes is limited by the mechanical properties of the cells, which will be largely dependent on the structure of the cell walls. Although it has long been observed that guard cells are anisotropic due to differential thickening and the orientation of cellulose microfibrils [2], our understanding of the composition of the cell wall that allows them to undergo repeated swelling and deflation remains surprisingly poor. Here, we show that the walls of guard cells are rich in un-esterified pectins. We identify a pectin methylesterase gene, PME6, which is highly expressed in guard cells and required for stomatal function. pme6-1 mutant guard cells have walls enriched in methyl-esterified pectin and show a decreased dynamic range in response to triggers of stomatal opening/closure, including elevated osmoticum, suggesting that abrogation of stomatal function reflects a mechanical change in the guard cell wall. Altered stomatal function leads to increased conductance and evaporative cooling, as well as decreased plant growth. The growth defect of the pme6-1 mutant is rescued by maintaining the plants in elevated CO2, substantiating gas exchange analyses, indicating that the mutant stomata can bestow an improved assimilation rate. Restoration of PME6 rescues guard cell wall pectin methyl-esterification status, stomatal function, and plant growth. Our results establish a link between gene expression in guard cells and their cell wall properties, with a corresponding effect on stomatal function and plant physiology.

AB - Stomatal opening and closure depends on changes in turgor pressure acting within guard cells to alter cell shape [1]. The extent of these shape changes is limited by the mechanical properties of the cells, which will be largely dependent on the structure of the cell walls. Although it has long been observed that guard cells are anisotropic due to differential thickening and the orientation of cellulose microfibrils [2], our understanding of the composition of the cell wall that allows them to undergo repeated swelling and deflation remains surprisingly poor. Here, we show that the walls of guard cells are rich in un-esterified pectins. We identify a pectin methylesterase gene, PME6, which is highly expressed in guard cells and required for stomatal function. pme6-1 mutant guard cells have walls enriched in methyl-esterified pectin and show a decreased dynamic range in response to triggers of stomatal opening/closure, including elevated osmoticum, suggesting that abrogation of stomatal function reflects a mechanical change in the guard cell wall. Altered stomatal function leads to increased conductance and evaporative cooling, as well as decreased plant growth. The growth defect of the pme6-1 mutant is rescued by maintaining the plants in elevated CO2, substantiating gas exchange analyses, indicating that the mutant stomata can bestow an improved assimilation rate. Restoration of PME6 rescues guard cell wall pectin methyl-esterification status, stomatal function, and plant growth. Our results establish a link between gene expression in guard cells and their cell wall properties, with a corresponding effect on stomatal function and plant physiology.

KW - Arabidopsis

KW - cell wall

KW - guard cell

KW - pectin

KW - stomata

U2 - 10.1016/j.cub.2016.08.021

DO - 10.1016/j.cub.2016.08.021

M3 - Journal article

C2 - 27720618

VL - 26

SP - 2899

EP - 2906

JO - Current biology : CB

JF - Current biology : CB

SN - 0960-9822

IS - 21

ER -