TY - JOUR

T1 - Stress responses and specific metal exclusion on mine soils based on germination and growth studies by Australian golden wattle

AU - Nirola, Ramkrishna

AU - Megharaj, Mallavarapu

AU - Aryal, Rupak

AU - Thavamani, Palanisami

AU - Ramdass, Kavitha

AU - Sarkar, Binoy

AU - Saint, Christopher

PY - 2016/12/31

Y1 - 2016/12/31

N2 - We reported the Australian golden wattle as a copper stabilizer in abandoned copper mine soils earlier. Here we investigate to confirm this plant’s suitability to grow on metal contaminated mine soils based on stress indication. The seeds of Acacia pycnantha collected from mining area were germinated after heat and no heat treatment on two types of irrigation. The daily irrigated and heat treated seeds gave up to 85% germination on sandy soil. The A. pycnantha was grown under greenhouse condition in six different soils collected from abandoned copper mine at Kapunda in South Australia. Among the six soil samples, soil-1 with the highest copper concentration produced 2.05 mmol g−1 tissue of proline. Proline expression was prominent in more saline soils (1, 5 and 6) having electrical conductivity (EC) 1184, 1364 and 1256 μS, respectively. Chlorophyll a, b and carotenoid levels in plants showed a gradually decreasing trend in all the soils as experiment progressed. The plants grown on soil sample-1, containing 4083 ± 103 mg kg−1 of copper resulted in 18 ± 2 mg kg−1 accumulation in its leaf. The calcium accumulation was significant up to 11648 ± 1209 mg kg−1 in leaf. Although pore water samples showed higher Cu concentration in soils, an increased mobility of arsenic and lead was observed in all the soil samples. Our experiment points out the need for proper monitoring of revegetation processes to avoid revegetation and reclamation failure.

AB - We reported the Australian golden wattle as a copper stabilizer in abandoned copper mine soils earlier. Here we investigate to confirm this plant’s suitability to grow on metal contaminated mine soils based on stress indication. The seeds of Acacia pycnantha collected from mining area were germinated after heat and no heat treatment on two types of irrigation. The daily irrigated and heat treated seeds gave up to 85% germination on sandy soil. The A. pycnantha was grown under greenhouse condition in six different soils collected from abandoned copper mine at Kapunda in South Australia. Among the six soil samples, soil-1 with the highest copper concentration produced 2.05 mmol g−1 tissue of proline. Proline expression was prominent in more saline soils (1, 5 and 6) having electrical conductivity (EC) 1184, 1364 and 1256 μS, respectively. Chlorophyll a, b and carotenoid levels in plants showed a gradually decreasing trend in all the soils as experiment progressed. The plants grown on soil sample-1, containing 4083 ± 103 mg kg−1 of copper resulted in 18 ± 2 mg kg−1 accumulation in its leaf. The calcium accumulation was significant up to 11648 ± 1209 mg kg−1 in leaf. Although pore water samples showed higher Cu concentration in soils, an increased mobility of arsenic and lead was observed in all the soil samples. Our experiment points out the need for proper monitoring of revegetation processes to avoid revegetation and reclamation failure.

KW - Acacia pycnantha

KW - Heavy metals

KW - Proline

KW - Phytoaccumulation

KW - Plant stress

KW - Uptake factors

U2 - 10.1016/j.ecolind.2016.06.062

DO - 10.1016/j.ecolind.2016.06.062

M3 - Journal article

VL - 71

SP - 113

EP - 122

JO - Ecological Indicators

JF - Ecological Indicators

SN - 1470-160X

ER -