Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Structural, surface, in vitro bacterial adhesion and biofilm formation analysis of three dental restorative composites
AU - Azam, M.T.
AU - Khan, A.S.
AU - Muzzafar, D.
AU - Faryal, R.
AU - Siddiqi, S.A.
AU - Ahmad, R.
AU - Chauhdry, A.A.
AU - Rehman, I.U.
PY - 2015
Y1 - 2015
N2 - This study was conducted to investigate the relationship between dental materials and bacterial adhesion on the grounds of their chemical composition and physical properties. Three commercially available dental restorative materials (Filtek™Z350, Filtek™P90 and Spectrum®TPH®) were structurally analyzed and their wettability and surface roughness were evaluated by using Fourier Transform Infrared Spectroscopy, Contact Angle Measurement and Atomic Force Microscopy, respectively. These materials were molded into discs and tested with three bacterial strains (Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia) for microbial attachment. The bacterial adhesion was observed at different time intervals, i.e., 0 h, 8 h, 24 h, 48 h and 72 h, along with Colony Forming Unit Count and Optical Density measurement of the media. It was found that all materials showed a degree of conversion with time intervals, i.e., 0 h, 8 h, 24 h, 48 h and 72 h, which led to the availability of functional groups (N-H and C-H) that might promote adhesion. The trend in difference in the extent of bacterial adhesion can be related to particle size, chemical composition and surface wettability of the dental materials. © 2015 by the authors.
AB - This study was conducted to investigate the relationship between dental materials and bacterial adhesion on the grounds of their chemical composition and physical properties. Three commercially available dental restorative materials (Filtek™Z350, Filtek™P90 and Spectrum®TPH®) were structurally analyzed and their wettability and surface roughness were evaluated by using Fourier Transform Infrared Spectroscopy, Contact Angle Measurement and Atomic Force Microscopy, respectively. These materials were molded into discs and tested with three bacterial strains (Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia) for microbial attachment. The bacterial adhesion was observed at different time intervals, i.e., 0 h, 8 h, 24 h, 48 h and 72 h, along with Colony Forming Unit Count and Optical Density measurement of the media. It was found that all materials showed a degree of conversion with time intervals, i.e., 0 h, 8 h, 24 h, 48 h and 72 h, which led to the availability of functional groups (N-H and C-H) that might promote adhesion. The trend in difference in the extent of bacterial adhesion can be related to particle size, chemical composition and surface wettability of the dental materials. © 2015 by the authors.
KW - Bacterial adhesion
KW - Dental composites
KW - Spectroscopic analysis
KW - Surface properties
KW - Wettability
KW - Adhesion
KW - Atomic force microscopy
KW - Bacteria
KW - Biomaterials
KW - Density measurement (optical)
KW - Dental materials
KW - Fourier transform infrared spectroscopy
KW - Functional groups
KW - Particle size
KW - Surface roughness
KW - Wetting
KW - Chemical compositions
KW - Colony forming units
KW - Degree of conversion
KW - Dental restorative composites
KW - Dental restorative materials
KW - Pseudomonas aeruginosa
KW - Staphylococcus aureus
U2 - 10.3390/ma8063221
DO - 10.3390/ma8063221
M3 - Journal article
VL - 8
SP - 3221
EP - 3237
JO - Materials
JF - Materials
SN - 1996-1944
IS - 6
ER -