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Structure of the keratan sulphate chains attached to fibromodulin isolated from bovine tracheal cartilage. Oligosaccharides generated by keratanase digestion.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Structure of the keratan sulphate chains attached to fibromodulin isolated from bovine tracheal cartilage. Oligosaccharides generated by keratanase digestion. / Lauder, R. M.; Huckerby, T. N.; Nieduszynski, I. A.
In: Biochemical Journal, Vol. 302, 1994, p. 417-423.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Lauder, RM, Huckerby, TN & Nieduszynski, IA 1994, 'Structure of the keratan sulphate chains attached to fibromodulin isolated from bovine tracheal cartilage. Oligosaccharides generated by keratanase digestion.', Biochemical Journal, vol. 302, pp. 417-423. <http://www.ncbi.nlm.nih.gov/sites/ppmc/articles/PMC1137244/>

APA

Lauder, R. M., Huckerby, T. N., & Nieduszynski, I. A. (1994). Structure of the keratan sulphate chains attached to fibromodulin isolated from bovine tracheal cartilage. Oligosaccharides generated by keratanase digestion. Biochemical Journal, 302, 417-423. http://www.ncbi.nlm.nih.gov/sites/ppmc/articles/PMC1137244/

Vancouver

Lauder RM, Huckerby TN, Nieduszynski IA. Structure of the keratan sulphate chains attached to fibromodulin isolated from bovine tracheal cartilage. Oligosaccharides generated by keratanase digestion. Biochemical Journal. 1994;302:417-423.

Author

Lauder, R. M. ; Huckerby, T. N. ; Nieduszynski, I. A. / Structure of the keratan sulphate chains attached to fibromodulin isolated from bovine tracheal cartilage. Oligosaccharides generated by keratanase digestion. In: Biochemical Journal. 1994 ; Vol. 302. pp. 417-423.

Bibtex

@article{c81f4f0f23674ac1a1687fea83d576fc,
title = "Structure of the keratan sulphate chains attached to fibromodulin isolated from bovine tracheal cartilage. Oligosaccharides generated by keratanase digestion.",
abstract = "The structure of the repeat region and chain caps of the N-linked keratan sulphate chains attached to bovine tracheal cartilage fibromodulin has been examined. The chains were fragmented by keratanase digestion, the resultant oligosaccharides isolated by strong anion-exchange chromatography, and their structures determined using high-field 1H-n.m.r. spectroscopy. The chains were found to possess the following general structure: [formula: see text] All of the capping oligosaccharides isolated terminate with alpha(2-3)-linked N-acetylneuraminic acid. No alpha(2-6)-linked N-acetylneuraminic acid chain terminators, nor any fucose, alpha (1-3)-linked to N-acetylglucosamine along the repeat region, were detected. This work demonstrates that the structure of the repeat region and chain caps of N-linked keratan sulphate attached to fibromodulin isolated from bovine tracheal cartilage is identical with that of O-linked keratan sulphate chains attached to aggrecan derived from non-articular cartilage.",
author = "Lauder, {R. M.} and Huckerby, {T. N.} and Nieduszynski, {I. A.}",
year = "1994",
language = "English",
volume = "302",
pages = "417--423",
journal = "Biochemical Journal",
issn = "0264-6021",
publisher = "Portland Press Ltd.",

}

RIS

TY - JOUR

T1 - Structure of the keratan sulphate chains attached to fibromodulin isolated from bovine tracheal cartilage. Oligosaccharides generated by keratanase digestion.

AU - Lauder, R. M.

AU - Huckerby, T. N.

AU - Nieduszynski, I. A.

PY - 1994

Y1 - 1994

N2 - The structure of the repeat region and chain caps of the N-linked keratan sulphate chains attached to bovine tracheal cartilage fibromodulin has been examined. The chains were fragmented by keratanase digestion, the resultant oligosaccharides isolated by strong anion-exchange chromatography, and their structures determined using high-field 1H-n.m.r. spectroscopy. The chains were found to possess the following general structure: [formula: see text] All of the capping oligosaccharides isolated terminate with alpha(2-3)-linked N-acetylneuraminic acid. No alpha(2-6)-linked N-acetylneuraminic acid chain terminators, nor any fucose, alpha (1-3)-linked to N-acetylglucosamine along the repeat region, were detected. This work demonstrates that the structure of the repeat region and chain caps of N-linked keratan sulphate attached to fibromodulin isolated from bovine tracheal cartilage is identical with that of O-linked keratan sulphate chains attached to aggrecan derived from non-articular cartilage.

AB - The structure of the repeat region and chain caps of the N-linked keratan sulphate chains attached to bovine tracheal cartilage fibromodulin has been examined. The chains were fragmented by keratanase digestion, the resultant oligosaccharides isolated by strong anion-exchange chromatography, and their structures determined using high-field 1H-n.m.r. spectroscopy. The chains were found to possess the following general structure: [formula: see text] All of the capping oligosaccharides isolated terminate with alpha(2-3)-linked N-acetylneuraminic acid. No alpha(2-6)-linked N-acetylneuraminic acid chain terminators, nor any fucose, alpha (1-3)-linked to N-acetylglucosamine along the repeat region, were detected. This work demonstrates that the structure of the repeat region and chain caps of N-linked keratan sulphate attached to fibromodulin isolated from bovine tracheal cartilage is identical with that of O-linked keratan sulphate chains attached to aggrecan derived from non-articular cartilage.

M3 - Journal article

VL - 302

SP - 417

EP - 423

JO - Biochemical Journal

JF - Biochemical Journal

SN - 0264-6021

ER -