Sub-micron poly(N-isopropylacrylamide) particles as temperature responsive vehicles for the detachment and delivery of human cells

Biomedical and Life Sciences

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https://doi.org/10.1039/b909656f
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Keywords

TERMINAL DIFFERENTIATION, DERMAL FIBROBLASTS, RGD PEPTIDES, INTEGRIN, ADHESION, ALPHA(5)BETA(1), FIBRONECTIN, RECOGNITION, CONETWORKS, ADSORPTION

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Sub-micron poly(N-isopropylacrylamide) particles as temperature responsive vehicles for the detachment and delivery of human cells. / Hopkins, Sally; Carter, Steven R.; Haycock, John W. et al.
In: Soft Matter, Vol. 5, No. 24, 2009, p. 4928-4937.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Bibtex

@article{2627e92ad3c541b292913abe7a4161b5,

title = "Sub-micron poly(N-isopropylacrylamide) particles as temperature responsive vehicles for the detachment and delivery of human cells",

abstract = "We describe the first example of particulate materials that can detach cultured cells and then release them intact in a temperature controlled manner. Topologically open microgels composed of water swollen highly branched polymers prepared from poly(N-isopropylacrylamide) (PNIPAM) were modified with a cell-adhesive peptide (GRGDS) to produce particles for gently detaching and then transferring cultured cells to new substrates. The particles bind to cell surface integrins on both dermal fibroblasts and endothelial cells and at temperatures above the lower critical solution temperature (34 degrees C) remove cells from their normal culture substrates. Brief (45 min) cooling of the resultant particle-cell dispersion to beneath 34 degrees C releases the cells to grow on new substrates. This avoids the need for trypsinisation to detach cells or centrifugation to collect cells post-detachment and offers a flexible approach to cell detachment and transport which is compatible with normal cell culture methodologies.",

keywords = "TERMINAL DIFFERENTIATION, DERMAL FIBROBLASTS, RGD PEPTIDES, INTEGRIN, ADHESION, ALPHA(5)BETA(1), FIBRONECTIN, RECOGNITION, CONETWORKS, ADSORPTION",

author = "Sally Hopkins and Carter, {Steven R.} and Haycock, {John W.} and Fullwood, {Nigel J.} and Sheila MacNeil and Stephen Rimmer",

year = "2009",

doi = "10.1039/b909656f",

language = "English",

volume = "5",

pages = "4928--4937",

journal = "Soft Matter",

issn = "1744-683X",

publisher = "Royal Society of Chemistry",

number = "24",

}

RIS

TY - JOUR

T1 - Sub-micron poly(N-isopropylacrylamide) particles as temperature responsive vehicles for the detachment and delivery of human cells

AU - Hopkins, Sally

AU - Carter, Steven R.

AU - Haycock, John W.

AU - Fullwood, Nigel J.

AU - MacNeil, Sheila

AU - Rimmer, Stephen

PY - 2009

Y1 - 2009

N2 - We describe the first example of particulate materials that can detach cultured cells and then release them intact in a temperature controlled manner. Topologically open microgels composed of water swollen highly branched polymers prepared from poly(N-isopropylacrylamide) (PNIPAM) were modified with a cell-adhesive peptide (GRGDS) to produce particles for gently detaching and then transferring cultured cells to new substrates. The particles bind to cell surface integrins on both dermal fibroblasts and endothelial cells and at temperatures above the lower critical solution temperature (34 degrees C) remove cells from their normal culture substrates. Brief (45 min) cooling of the resultant particle-cell dispersion to beneath 34 degrees C releases the cells to grow on new substrates. This avoids the need for trypsinisation to detach cells or centrifugation to collect cells post-detachment and offers a flexible approach to cell detachment and transport which is compatible with normal cell culture methodologies.

AB - We describe the first example of particulate materials that can detach cultured cells and then release them intact in a temperature controlled manner. Topologically open microgels composed of water swollen highly branched polymers prepared from poly(N-isopropylacrylamide) (PNIPAM) were modified with a cell-adhesive peptide (GRGDS) to produce particles for gently detaching and then transferring cultured cells to new substrates. The particles bind to cell surface integrins on both dermal fibroblasts and endothelial cells and at temperatures above the lower critical solution temperature (34 degrees C) remove cells from their normal culture substrates. Brief (45 min) cooling of the resultant particle-cell dispersion to beneath 34 degrees C releases the cells to grow on new substrates. This avoids the need for trypsinisation to detach cells or centrifugation to collect cells post-detachment and offers a flexible approach to cell detachment and transport which is compatible with normal cell culture methodologies.

KW - TERMINAL DIFFERENTIATION

KW - DERMAL FIBROBLASTS

KW - RGD PEPTIDES

KW - INTEGRIN

KW - ADHESION

KW - ALPHA(5)BETA(1)

KW - FIBRONECTIN

KW - RECOGNITION

KW - CONETWORKS

KW - ADSORPTION

U2 - 10.1039/b909656f

DO - 10.1039/b909656f

M3 - Journal article

VL - 5

SP - 4928

EP - 4937

JO - Soft Matter

JF - Soft Matter

SN - 1744-683X

IS - 24

ER -

Research

Text available via DOI:

Keywords