TY - JOUR

T1 - Substrate contribution on free radical scavenging capacity of carotenoid extracts produced from Blakeslea trispora cultures

AU - Papaioannou, Emmanouil H.

AU - Stoforos, Nikolaos G.

AU - Liakopoulou-Kyriakides, Maria

PY - 2011/4

Y1 - 2011/4

N2 - Blakeslea trispora produces carotenoids mixtures consisting mainly of lycopene, γ-carotene and β-carotene, together with trace amounts of other carotenoid precursors. The yield of these carotenoids and their composition are greatly affected by culture substrate. The scavenging capacity of carotenoids extract from cultures of B. trispora growing in various substrates was estimated using the 2,2-diphenyl-1-picrylhydrazyl method. Fractions enriched in β-carotene, γ-carotene and lycopene, obtained after column chromatography in alumina basic II, were also examined. Substrates containing starch and oils mixture, Ni2+, and that with pantothenic acid presented higher antioxidant activity. An increase in the antioxidant activity of the crude carotenoid extract compared to that of the isolated fractions enriched in β-carotene, γ-carotene and lycopene respectively, observed in most samples, indicated a possible synergistic effect. The results are of interest and by expanding this study to more substrates and other microorganisms- producing antioxidants, a formulation of extract with high free radical scavenging potential could be produced.

AB - Blakeslea trispora produces carotenoids mixtures consisting mainly of lycopene, γ-carotene and β-carotene, together with trace amounts of other carotenoid precursors. The yield of these carotenoids and their composition are greatly affected by culture substrate. The scavenging capacity of carotenoids extract from cultures of B. trispora growing in various substrates was estimated using the 2,2-diphenyl-1-picrylhydrazyl method. Fractions enriched in β-carotene, γ-carotene and lycopene, obtained after column chromatography in alumina basic II, were also examined. Substrates containing starch and oils mixture, Ni2+, and that with pantothenic acid presented higher antioxidant activity. An increase in the antioxidant activity of the crude carotenoid extract compared to that of the isolated fractions enriched in β-carotene, γ-carotene and lycopene respectively, observed in most samples, indicated a possible synergistic effect. The results are of interest and by expanding this study to more substrates and other microorganisms- producing antioxidants, a formulation of extract with high free radical scavenging potential could be produced.

KW - β-Carotene

KW - γ-Carotene

KW - Blakeslea trispora

KW - Carotenoids

KW - DPPH

KW - Lycopene

U2 - 10.1007/s11274-010-0527-z

DO - 10.1007/s11274-010-0527-z

M3 - Journal article

AN - SCOPUS:79952575752

VL - 27

SP - 851

EP - 858

JO - World Journal of Microbiology and Biotechnology

JF - World Journal of Microbiology and Biotechnology

SN - 0959-3993

IS - 4

ER -