Home > Research > Publications & Outputs > Survival of nonculturable Aeromonas salmonicida...

Research

Links

Text available via DOI:

View graph of relations

Survival of nonculturable Aeromonas salmonicida in lake water

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Published

Standard

Survival of nonculturable Aeromonas salmonicida in lake water. / Morgan, J. A.W.; Rhodes, G.; Pickup, R. W.
In: Applied and Environmental Microbiology, Vol. 59, No. 3, 01.03.1993, p. 874-880.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Morgan, JAW, Rhodes, G & Pickup, RW 1993, 'Survival of nonculturable Aeromonas salmonicida in lake water', Applied and Environmental Microbiology, vol. 59, no. 3, pp. 874-880. https://doi.org/10.1128/aem.59.3.874-880.1993

APA

Morgan, J. A. W., Rhodes, G., & Pickup, R. W. (1993). Survival of nonculturable Aeromonas salmonicida in lake water. Applied and Environmental Microbiology, 59(3), 874-880. https://doi.org/10.1128/aem.59.3.874-880.1993

Vancouver

Morgan JAW, Rhodes G, Pickup RW. Survival of nonculturable Aeromonas salmonicida in lake water. Applied and Environmental Microbiology. 1993 Mar 1;59(3):874-880. doi: 10.1128/aem.59.3.874-880.1993

Author

Morgan, J. A.W. ; Rhodes, G. ; Pickup, R. W. / Survival of nonculturable Aeromonas salmonicida in lake water. In: Applied and Environmental Microbiology. 1993 ; Vol. 59, No. 3. pp. 874-880.

Bibtex

@article{c5d0d8357a2d47b8a4c7b583c2a76b59,
title = "Survival of nonculturable Aeromonas salmonicida in lake water",
abstract = "The survival of Aeromonas salmonicida subsp. salmonicida was investigated in sterile and untreated lake water. In sterile lake water (filtered and autoclaved), it was found that cells of A. salmonicida entered a nonculturable but viable condition. Viability was determined by flow cytometry with the dye rhodamine 123, which is taken up and maintained within cells with a membrane potential. For survival studies in untreated lake water, A. salmonicida was marked with the xylE gene by using the plasmid pLV1013. Marked cells were detected by growth on tryptone soy agar and tryptone soy agar supplemented with kanamycin. Cells were also detected by polymerase chain reaction DNA amplification of the xylE gene and a chromosomal DNA fragment specific for A. salmonicida (pLV1013). The results indicated that A. salmonicida entered a nonculturable condition in untreated lake water over a 21-day study. The viability of nonculturable cells could not be determined in mixed samples; however, the presence of nonculturable cells containing both chromosomal and plasmid DNA was confirmed.",
author = "Morgan, {J. A.W.} and G. Rhodes and Pickup, {R. W.}",
year = "1993",
month = mar,
day = "1",
doi = "10.1128/aem.59.3.874-880.1993",
language = "English",
volume = "59",
pages = "874--880",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "3",

}

RIS

TY - JOUR

T1 - Survival of nonculturable Aeromonas salmonicida in lake water

AU - Morgan, J. A.W.

AU - Rhodes, G.

AU - Pickup, R. W.

PY - 1993/3/1

Y1 - 1993/3/1

N2 - The survival of Aeromonas salmonicida subsp. salmonicida was investigated in sterile and untreated lake water. In sterile lake water (filtered and autoclaved), it was found that cells of A. salmonicida entered a nonculturable but viable condition. Viability was determined by flow cytometry with the dye rhodamine 123, which is taken up and maintained within cells with a membrane potential. For survival studies in untreated lake water, A. salmonicida was marked with the xylE gene by using the plasmid pLV1013. Marked cells were detected by growth on tryptone soy agar and tryptone soy agar supplemented with kanamycin. Cells were also detected by polymerase chain reaction DNA amplification of the xylE gene and a chromosomal DNA fragment specific for A. salmonicida (pLV1013). The results indicated that A. salmonicida entered a nonculturable condition in untreated lake water over a 21-day study. The viability of nonculturable cells could not be determined in mixed samples; however, the presence of nonculturable cells containing both chromosomal and plasmid DNA was confirmed.

AB - The survival of Aeromonas salmonicida subsp. salmonicida was investigated in sterile and untreated lake water. In sterile lake water (filtered and autoclaved), it was found that cells of A. salmonicida entered a nonculturable but viable condition. Viability was determined by flow cytometry with the dye rhodamine 123, which is taken up and maintained within cells with a membrane potential. For survival studies in untreated lake water, A. salmonicida was marked with the xylE gene by using the plasmid pLV1013. Marked cells were detected by growth on tryptone soy agar and tryptone soy agar supplemented with kanamycin. Cells were also detected by polymerase chain reaction DNA amplification of the xylE gene and a chromosomal DNA fragment specific for A. salmonicida (pLV1013). The results indicated that A. salmonicida entered a nonculturable condition in untreated lake water over a 21-day study. The viability of nonculturable cells could not be determined in mixed samples; however, the presence of nonculturable cells containing both chromosomal and plasmid DNA was confirmed.

U2 - 10.1128/aem.59.3.874-880.1993

DO - 10.1128/aem.59.3.874-880.1993

M3 - Journal article

C2 - 8481011

AN - SCOPUS:0027338866

VL - 59

SP - 874

EP - 880

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 3

ER -