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Synchronization of Trypanosoma brucei by Counter-Flow Centrifugal Elutriation

Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSN › Chapter (peer-reviewed) › peer-review

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Synchronization of Trypanosoma brucei by Counter-Flow Centrifugal Elutriation. / Benz, Corinna; Urbaniak, Michael D.
Cell-Cycle Synchronization: Methods and Protocols. ed. / Zhixiang Wang. New York: Humana Press, 2022. p. 137-144 (Methods in Molecular Biology; Vol. 2579).

Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSN › Chapter (peer-reviewed) › peer-review

Harvard

Benz, C & Urbaniak, MD 2022, Synchronization of Trypanosoma brucei by Counter-Flow Centrifugal Elutriation. in Z Wang (ed.), Cell-Cycle Synchronization: Methods and Protocols. Methods in Molecular Biology, vol. 2579, Humana Press, New York, pp. 137-144. https://doi.org/10.1007/978-1-0716-2736-5_11

APA

Benz, C., & Urbaniak, M. D. (2022). Synchronization of Trypanosoma brucei by Counter-Flow Centrifugal Elutriation. In Z. Wang (Ed.), Cell-Cycle Synchronization: Methods and Protocols (pp. 137-144). (Methods in Molecular Biology; Vol. 2579). Humana Press. https://doi.org/10.1007/978-1-0716-2736-5_11

Vancouver

Benz C, Urbaniak MD. Synchronization of Trypanosoma brucei by Counter-Flow Centrifugal Elutriation. In Wang Z, editor, Cell-Cycle Synchronization: Methods and Protocols. New York: Humana Press. 2022. p. 137-144. (Methods in Molecular Biology). doi: 10.1007/978-1-0716-2736-5_11

Author

Benz, Corinna ; Urbaniak, Michael D. / Synchronization of Trypanosoma brucei by Counter-Flow Centrifugal Elutriation. Cell-Cycle Synchronization: Methods and Protocols. editor / Zhixiang Wang. New York : Humana Press, 2022. pp. 137-144 (Methods in Molecular Biology).

Bibtex

@inbook{2d4df850a2f6492dbb3b6436e76af8c3,
title = "Synchronization of Trypanosoma brucei by Counter-Flow Centrifugal Elutriation",
abstract = "Centrifugal counter-flow elutriation is a non-invasive technique that separates cells based on their hydrodynamic volume in a specialized centrifugation chamber that allows the application of a counter-flow of buffer to oppose sedimentation. Here, we report a centrifugal counter-flow elutriation protocol for Trypanosoma brucei cells that is able to rapidly isolate highly enriched G1 subpopulations (>95%) of synchronized cells. The cells obtained are viable and proliferate without lag, allowing subsequent cell cycle phases to be obtained by continued culture. The synchronized cell cultures obtained by this process have uniform DNA content, a narrow size distribution, undergo synchronous division, and maintain synchrony into subsequent cell cycles.",
keywords = "Centrifugation - methods, Elutriation, Cell Culture Techniques, Flow cytometry, Trypanosoma brucei, Synchronization, Cell cycle, Cell Cycle - genetics, Cell Separation - methods, Trypanosoma brucei brucei, Cell Division",
author = "Corinna Benz and Urbaniak, {Michael D}",
year = "2022",
month = sep,
day = "1",
doi = "10.1007/978-1-0716-2736-5_11",
language = "English",
isbn = "9781071627358",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "137--144",
editor = "Zhixiang Wang",
booktitle = "Cell-Cycle Synchronization",
address = "United States",

}

RIS

TY - CHAP

T1 - Synchronization of Trypanosoma brucei by Counter-Flow Centrifugal Elutriation

AU - Benz, Corinna

AU - Urbaniak, Michael D

PY - 2022/9/1

Y1 - 2022/9/1

N2 - Centrifugal counter-flow elutriation is a non-invasive technique that separates cells based on their hydrodynamic volume in a specialized centrifugation chamber that allows the application of a counter-flow of buffer to oppose sedimentation. Here, we report a centrifugal counter-flow elutriation protocol for Trypanosoma brucei cells that is able to rapidly isolate highly enriched G1 subpopulations (>95%) of synchronized cells. The cells obtained are viable and proliferate without lag, allowing subsequent cell cycle phases to be obtained by continued culture. The synchronized cell cultures obtained by this process have uniform DNA content, a narrow size distribution, undergo synchronous division, and maintain synchrony into subsequent cell cycles.

AB - Centrifugal counter-flow elutriation is a non-invasive technique that separates cells based on their hydrodynamic volume in a specialized centrifugation chamber that allows the application of a counter-flow of buffer to oppose sedimentation. Here, we report a centrifugal counter-flow elutriation protocol for Trypanosoma brucei cells that is able to rapidly isolate highly enriched G1 subpopulations (>95%) of synchronized cells. The cells obtained are viable and proliferate without lag, allowing subsequent cell cycle phases to be obtained by continued culture. The synchronized cell cultures obtained by this process have uniform DNA content, a narrow size distribution, undergo synchronous division, and maintain synchrony into subsequent cell cycles.

KW - Centrifugation - methods

KW - Elutriation

KW - Cell Culture Techniques

KW - Flow cytometry

KW - Trypanosoma brucei

KW - Synchronization

KW - Cell cycle

KW - Cell Cycle - genetics

KW - Cell Separation - methods

KW - Trypanosoma brucei brucei

KW - Cell Division

U2 - 10.1007/978-1-0716-2736-5_11

DO - 10.1007/978-1-0716-2736-5_11

M3 - Chapter (peer-reviewed)

C2 - 36045204

SN - 9781071627358

T3 - Methods in Molecular Biology

SP - 137

EP - 144

BT - Cell-Cycle Synchronization

A2 - Wang, Zhixiang

PB - Humana Press

CY - New York

ER -