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Synergistic effects on gene delivery - co-formulation of small disulfide-linked dendritic polycations with Lipofectamine 2000 (TM)

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Synergistic effects on gene delivery - co-formulation of small disulfide-linked dendritic polycations with Lipofectamine 2000 (TM). / Hardy, John G.; Love, Christine S.; Gabrielson, Nathan P.; Pack, Daniel W.; Smith, David K.

In: Organic and Biomolecular Chemistry , Vol. 7, No. 4, 21.02.2009, p. 789-793.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Hardy, JG, Love, CS, Gabrielson, NP, Pack, DW & Smith, DK 2009, 'Synergistic effects on gene delivery - co-formulation of small disulfide-linked dendritic polycations with Lipofectamine 2000 (TM)', Organic and Biomolecular Chemistry , vol. 7, no. 4, pp. 789-793. https://doi.org/10.1039/b818469k

APA

Hardy, J. G., Love, C. S., Gabrielson, N. P., Pack, D. W., & Smith, D. K. (2009). Synergistic effects on gene delivery - co-formulation of small disulfide-linked dendritic polycations with Lipofectamine 2000 (TM). Organic and Biomolecular Chemistry , 7(4), 789-793. https://doi.org/10.1039/b818469k

Vancouver

Author

Hardy, John G. ; Love, Christine S. ; Gabrielson, Nathan P. ; Pack, Daniel W. ; Smith, David K. / Synergistic effects on gene delivery - co-formulation of small disulfide-linked dendritic polycations with Lipofectamine 2000 (TM). In: Organic and Biomolecular Chemistry . 2009 ; Vol. 7, No. 4. pp. 789-793.

Bibtex

@article{809975a57c344ce19b23474f0e85f085,
title = "Synergistic effects on gene delivery - co-formulation of small disulfide-linked dendritic polycations with Lipofectamine 2000 (TM)",
abstract = "This paper describes the application of gene delivery vectors based on connecting together two well-defined low-generation poly(L-lysine) (PLL) dendrons using a disulfide-containing linker unit. We report that the transfection ability of these vectors in their own right is relatively low, because the low-generation number limits the endosomal buffering capacity. Importantly, however, we demonstrate that when applied in combination with Lipofectamine 2000 (TM), a vector from the cationic lipid family, these small cationic additives significantly enhance the levels of gene delivery (up to four-fold). Notably, the cationic additives have no effect on the levels of transfection observed with a cationic polymer, such as DEAE dextran. We therefore argue that the synergistic effects observed with Lipofectamine 2000 (TM) arise as a result of combining the delivery advantages of two different classes of vector within a single formulation, with our dendritic additives providing a degree of pH buffering within the endosome. As such, the data we present indicate that small dendritic structures, although previously largely overlooked for gene delivery owing to their inability to transfect in their own right, may actually be useful well-defined additives to well-established vector systems in order to enhance the gene delivery payload.",
keywords = "IN-VITRO, DNA-BINDING, MULTIVALENT DENDRONS, PLASMID DNA, GLYCOL)-BLOCK-POLY(L-LYSINE) DENDRIMER, ANTISENSE OLIGONUCLEOTIDES, GOLD NANOPARTICLES, NONVIRAL CARRIERS, LYSINE DENDRIMERS, CATIONIC POLYMER, Chemistry(all), Molecular Medicine, Biomaterials",
author = "Hardy, {John G.} and Love, {Christine S.} and Gabrielson, {Nathan P.} and Pack, {Daniel W.} and Smith, {David K.}",
year = "2009",
month = feb,
day = "21",
doi = "10.1039/b818469k",
language = "English",
volume = "7",
pages = "789--793",
journal = "Organic and Biomolecular Chemistry ",
issn = "1477-0520",
publisher = "Royal Society of Chemistry",
number = "4",

}

RIS

TY - JOUR

T1 - Synergistic effects on gene delivery - co-formulation of small disulfide-linked dendritic polycations with Lipofectamine 2000 (TM)

AU - Hardy, John G.

AU - Love, Christine S.

AU - Gabrielson, Nathan P.

AU - Pack, Daniel W.

AU - Smith, David K.

PY - 2009/2/21

Y1 - 2009/2/21

N2 - This paper describes the application of gene delivery vectors based on connecting together two well-defined low-generation poly(L-lysine) (PLL) dendrons using a disulfide-containing linker unit. We report that the transfection ability of these vectors in their own right is relatively low, because the low-generation number limits the endosomal buffering capacity. Importantly, however, we demonstrate that when applied in combination with Lipofectamine 2000 (TM), a vector from the cationic lipid family, these small cationic additives significantly enhance the levels of gene delivery (up to four-fold). Notably, the cationic additives have no effect on the levels of transfection observed with a cationic polymer, such as DEAE dextran. We therefore argue that the synergistic effects observed with Lipofectamine 2000 (TM) arise as a result of combining the delivery advantages of two different classes of vector within a single formulation, with our dendritic additives providing a degree of pH buffering within the endosome. As such, the data we present indicate that small dendritic structures, although previously largely overlooked for gene delivery owing to their inability to transfect in their own right, may actually be useful well-defined additives to well-established vector systems in order to enhance the gene delivery payload.

AB - This paper describes the application of gene delivery vectors based on connecting together two well-defined low-generation poly(L-lysine) (PLL) dendrons using a disulfide-containing linker unit. We report that the transfection ability of these vectors in their own right is relatively low, because the low-generation number limits the endosomal buffering capacity. Importantly, however, we demonstrate that when applied in combination with Lipofectamine 2000 (TM), a vector from the cationic lipid family, these small cationic additives significantly enhance the levels of gene delivery (up to four-fold). Notably, the cationic additives have no effect on the levels of transfection observed with a cationic polymer, such as DEAE dextran. We therefore argue that the synergistic effects observed with Lipofectamine 2000 (TM) arise as a result of combining the delivery advantages of two different classes of vector within a single formulation, with our dendritic additives providing a degree of pH buffering within the endosome. As such, the data we present indicate that small dendritic structures, although previously largely overlooked for gene delivery owing to their inability to transfect in their own right, may actually be useful well-defined additives to well-established vector systems in order to enhance the gene delivery payload.

KW - IN-VITRO

KW - DNA-BINDING

KW - MULTIVALENT DENDRONS

KW - PLASMID DNA

KW - GLYCOL)-BLOCK-POLY(L-LYSINE) DENDRIMER

KW - ANTISENSE OLIGONUCLEOTIDES

KW - GOLD NANOPARTICLES

KW - NONVIRAL CARRIERS

KW - LYSINE DENDRIMERS

KW - CATIONIC POLYMER

KW - Chemistry(all)

KW - Molecular Medicine

KW - Biomaterials

U2 - 10.1039/b818469k

DO - 10.1039/b818469k

M3 - Journal article

VL - 7

SP - 789

EP - 793

JO - Organic and Biomolecular Chemistry

JF - Organic and Biomolecular Chemistry

SN - 1477-0520

IS - 4

ER -