Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Synthesis and in-vitro cytotoxicity analysis of microwave irradiated nano-apatites
AU - Nazir, R.
AU - Samad Khan, A.
AU - Ahmed, A.
AU - Ur-Rehman, A.
AU - Anwar Chaudhry, A.
AU - Ur Rehman, I.
AU - Wong, F.S.L.
PY - 2013
Y1 - 2013
N2 - Nano-sized calcium deficient apatite (CDA) micelles were synthesized through microwave assisted the wet precipitation technique. Cetyltrimethylammonium bromide (CTAB) was employed as surface template to furnish the CDA particles with tailored size and shape. As-precipitated CDA was heat treated to observe the effect of heat treatment temperature on the interatomic rearrangement of entities within the apatite lattice. This transformation is responsible for conversion of CDA to β-tricalcium phosphate (β-TCP) at specific temperature. The phase purity, particles size, morphology and transformation kinetics were analyzed using X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM) and Thermogravimetric Analysis (TGA). In-vitro studies were performed on β-TCP with three cell lines: osteoblasts, HeLa, and SF 767. The results showed that nano-sized particles were successfully synthesized in short time. The cells had appreciable proliferation/attachment on the surface of these nano-particles. It is concluded that the microwave irradiated synthesized β-TCP has good capacity in terms of biocompatibility and has the potential to be used in hard tissue regeneration applications. © 2012 Elsevier Ltd and Techna Group S.r.l.
AB - Nano-sized calcium deficient apatite (CDA) micelles were synthesized through microwave assisted the wet precipitation technique. Cetyltrimethylammonium bromide (CTAB) was employed as surface template to furnish the CDA particles with tailored size and shape. As-precipitated CDA was heat treated to observe the effect of heat treatment temperature on the interatomic rearrangement of entities within the apatite lattice. This transformation is responsible for conversion of CDA to β-tricalcium phosphate (β-TCP) at specific temperature. The phase purity, particles size, morphology and transformation kinetics were analyzed using X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM) and Thermogravimetric Analysis (TGA). In-vitro studies were performed on β-TCP with three cell lines: osteoblasts, HeLa, and SF 767. The results showed that nano-sized particles were successfully synthesized in short time. The cells had appreciable proliferation/attachment on the surface of these nano-particles. It is concluded that the microwave irradiated synthesized β-TCP has good capacity in terms of biocompatibility and has the potential to be used in hard tissue regeneration applications. © 2012 Elsevier Ltd and Techna Group S.r.l.
KW - Cytotoxicity
KW - Microwave assisted synthesis
KW - Nano-particles
KW - Tricalcium phosphate (TCP)
KW - Calcium deficient apatites
KW - Cell lines
KW - Cetyltrimethylammonium bromide
KW - Effect of heat treatments
KW - In-vitro
KW - Microwave assisted
KW - Nano-apatites
KW - Nano-sized particles
KW - Particles sizes
KW - Phase purity
KW - Size and shape
KW - Surface templates
KW - Transformation kinetics
KW - Tricalcium phosphates
KW - Wet precipitation
KW - Apatite
KW - Biocompatibility
KW - Cell culture
KW - Fourier transform infrared spectroscopy
KW - Microwaves
KW - Nanoparticles
KW - Particle size
KW - Scanning electron microscopy
KW - Thermogravimetric analysis
KW - X ray diffraction
KW - Synthesis (chemical)
U2 - 10.1016/j.ceramint.2012.11.017
DO - 10.1016/j.ceramint.2012.11.017
M3 - Journal article
VL - 39
SP - 4339
EP - 4347
JO - Ceramics International
JF - Ceramics International
SN - 0272-8842
IS - 4
ER -