Temperature-dependent Fourier transform infrared spectra of phospholipid bilayers: melittin- and cholesterol-induced effects on molecular organization.

Lancaster University

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https://doi.org/10.1016/0924-2031(93)87039-V
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Keywords

Infrared spectrometry, Cholesterol, Fourier transform, Melittin, Molecular organization, Phospholipid bilayers

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Temperature-dependent Fourier transform infrared spectra of phospholipid bilayers: melittin- and cholesterol-induced effects on molecular organization. / Akyüz, Sevim; Davies, J. Eric D.
In: Vibrational Spectroscopy, Vol. 4, No. 2, 14.01.1993, p. 199-206.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Bibtex

@article{776dbbd009e54666a7ba9752f248bae8,

title = "Temperature-dependent Fourier transform infrared spectra of phospholipid bilayers: melittin- and cholesterol-induced effects on molecular organization.",

abstract = "The perturbations induced by melittin and cholesterol on the molecular organization of dimyristoyl- -α-phosphatidylcholine (DMPC) bilayers, depending on the temperature and concentrations of both melittin and cholesterol, were studied using FT-IR spectrometry. Analysis of the amide I and amide II bands of melittin indicates that melittin exists as a monomer at all temperatures in the DMPC-melittin system (lipid:protein molar ratio R = 14:1) and in DMPC-cholesterol-melittin dispersions (R = 14 and 25) for low cholesterol concentrations (10 mol%). However in the DMPC-cholesterol-melittin systems with high cholesterol (20 mol%) and high melittin (R = 14) concentrations, melittin is found to be in a tetrameric form at T > Tm. The melittin-induced effects in DMPC bilayers containing a low melittin concentration (R = 25) are found to decrease on addition of cholesterol (10 or 20 mol%). Cholesterol, however, has the opposite effect in DMPC-cholesterol-melittin systems containing high cholesterol (20 mol%) and high melittin concentrations (R = 14), i.e., and increased lipid-protein interaction. Cholesterol- and melittin-induced effects on the spectral features of the phospholipid are discussed.",

keywords = "Infrared spectrometry, Cholesterol, Fourier transform, Melittin, Molecular organization, Phospholipid bilayers",

author = "Sevim Aky{\"u}z and Davies, {J. Eric D.}",

year = "1993",

month = jan,

day = "14",

doi = "10.1016/0924-2031(93)87039-V",

language = "English",

volume = "4",

pages = "199--206",

journal = "Vibrational Spectroscopy",

publisher = "Elsevier",

number = "2",

}

RIS

TY - JOUR

T1 - Temperature-dependent Fourier transform infrared spectra of phospholipid bilayers: melittin- and cholesterol-induced effects on molecular organization.

AU - Akyüz, Sevim

AU - Davies, J. Eric D.

PY - 1993/1/14

Y1 - 1993/1/14

N2 - The perturbations induced by melittin and cholesterol on the molecular organization of dimyristoyl- -α-phosphatidylcholine (DMPC) bilayers, depending on the temperature and concentrations of both melittin and cholesterol, were studied using FT-IR spectrometry. Analysis of the amide I and amide II bands of melittin indicates that melittin exists as a monomer at all temperatures in the DMPC-melittin system (lipid:protein molar ratio R = 14:1) and in DMPC-cholesterol-melittin dispersions (R = 14 and 25) for low cholesterol concentrations (10 mol%). However in the DMPC-cholesterol-melittin systems with high cholesterol (20 mol%) and high melittin (R = 14) concentrations, melittin is found to be in a tetrameric form at T > Tm. The melittin-induced effects in DMPC bilayers containing a low melittin concentration (R = 25) are found to decrease on addition of cholesterol (10 or 20 mol%). Cholesterol, however, has the opposite effect in DMPC-cholesterol-melittin systems containing high cholesterol (20 mol%) and high melittin concentrations (R = 14), i.e., and increased lipid-protein interaction. Cholesterol- and melittin-induced effects on the spectral features of the phospholipid are discussed.

AB - The perturbations induced by melittin and cholesterol on the molecular organization of dimyristoyl- -α-phosphatidylcholine (DMPC) bilayers, depending on the temperature and concentrations of both melittin and cholesterol, were studied using FT-IR spectrometry. Analysis of the amide I and amide II bands of melittin indicates that melittin exists as a monomer at all temperatures in the DMPC-melittin system (lipid:protein molar ratio R = 14:1) and in DMPC-cholesterol-melittin dispersions (R = 14 and 25) for low cholesterol concentrations (10 mol%). However in the DMPC-cholesterol-melittin systems with high cholesterol (20 mol%) and high melittin (R = 14) concentrations, melittin is found to be in a tetrameric form at T > Tm. The melittin-induced effects in DMPC bilayers containing a low melittin concentration (R = 25) are found to decrease on addition of cholesterol (10 or 20 mol%). Cholesterol, however, has the opposite effect in DMPC-cholesterol-melittin systems containing high cholesterol (20 mol%) and high melittin concentrations (R = 14), i.e., and increased lipid-protein interaction. Cholesterol- and melittin-induced effects on the spectral features of the phospholipid are discussed.

KW - Infrared spectrometry

KW - Cholesterol

KW - Fourier transform

KW - Melittin

KW - Molecular organization

KW - Phospholipid bilayers

U2 - 10.1016/0924-2031(93)87039-V

DO - 10.1016/0924-2031(93)87039-V

M3 - Journal article

VL - 4

SP - 199

EP - 206

JO - Vibrational Spectroscopy

JF - Vibrational Spectroscopy

IS - 2

ER -

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