Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Temporal variation in CB2R levels following T lymphocyte activation : evidence that cannabinoids modulate CXCL12-induced chemotaxis.
AU - Coopman, Karen
AU - Smith, Laura D.
AU - Wright, Karen L.
AU - Ward, Stephen G.
PY - 2007/3
Y1 - 2007/3
N2 - Cannabinoids have long been proposed to affect the immune system, especially as one of the cannabinoid receptors, the cannabinoid receptor-2 (CB2R) has been found almost exclusively on immune cells. Here, using human in vitro activated peripheral blood-derived T lymphocytes we investigated the long-term changes in cannabinoid receptor protein expression following cellular activation and the effects of cannabinoids on migration. We report that resting T lymphocytes do not detectably express either the cannabinoid receptor-1 (CB1R) or CB2R at the protein level. However, CB2R protein expression is upregulated in a biphasic manner in T lymphocytes following activation by superantigen. The cannabinoids 2-AG and JWH-133 were found to elicit activation of downstream biochemical effectors (as assessed by the phosphorylation of the ERK1/2 MAP kinases). Neither 2-AG nor JWH-133 induced chemotaxis in day 5 activated T lymphocytes, when receptor expression was at its highest. Interestingly, both 2-AG and JWH-133 inhibited CXCL12-induced chemotaxis, suggesting a modulatory role for cannabinoids in activated T lymphocytes. Keywords: Cannabinoid; Receptors; T lymphocyte; Migration Abbreviations: 2-AG, 2-arachidonoylglycerol; AEA, arachidonoylethanolamide; CB1R, cannabinoid receptor-1; CB2R, cannabinoid receptor-2; ERK, extracellular signal-regulated kinase; FAAH, fatty acid amide hyrdrolase; GPCR, G-protein coupled receptor; MAGL, monoacylglycerol lipase; PBMC, peripheral blood mononuclear cell; PMA, phorbyl 13-myristate 12-acetate; PI3K, phosphoinositide 3-kinase; PTX, pertussis toxin; SEB, Staphyloccocal enterotoxin B; WCL, whole cell lysate.
AB - Cannabinoids have long been proposed to affect the immune system, especially as one of the cannabinoid receptors, the cannabinoid receptor-2 (CB2R) has been found almost exclusively on immune cells. Here, using human in vitro activated peripheral blood-derived T lymphocytes we investigated the long-term changes in cannabinoid receptor protein expression following cellular activation and the effects of cannabinoids on migration. We report that resting T lymphocytes do not detectably express either the cannabinoid receptor-1 (CB1R) or CB2R at the protein level. However, CB2R protein expression is upregulated in a biphasic manner in T lymphocytes following activation by superantigen. The cannabinoids 2-AG and JWH-133 were found to elicit activation of downstream biochemical effectors (as assessed by the phosphorylation of the ERK1/2 MAP kinases). Neither 2-AG nor JWH-133 induced chemotaxis in day 5 activated T lymphocytes, when receptor expression was at its highest. Interestingly, both 2-AG and JWH-133 inhibited CXCL12-induced chemotaxis, suggesting a modulatory role for cannabinoids in activated T lymphocytes. Keywords: Cannabinoid; Receptors; T lymphocyte; Migration Abbreviations: 2-AG, 2-arachidonoylglycerol; AEA, arachidonoylethanolamide; CB1R, cannabinoid receptor-1; CB2R, cannabinoid receptor-2; ERK, extracellular signal-regulated kinase; FAAH, fatty acid amide hyrdrolase; GPCR, G-protein coupled receptor; MAGL, monoacylglycerol lipase; PBMC, peripheral blood mononuclear cell; PMA, phorbyl 13-myristate 12-acetate; PI3K, phosphoinositide 3-kinase; PTX, pertussis toxin; SEB, Staphyloccocal enterotoxin B; WCL, whole cell lysate.
U2 - 10.1016/j.intimp.2006.11.008
DO - 10.1016/j.intimp.2006.11.008
M3 - Journal article
VL - 7
SP - 360
EP - 371
JO - International Immunopharmacology
JF - International Immunopharmacology
SN - 1567-5769
IS - 3
ER -