Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - The Acer gene of Drosophila codes for an angiotensin-converting enzyme homologue.
AU - Taylor, C. A. M.
AU - Coates, David
AU - Shirras, Alan D.
PY - 1996/11
Y1 - 1996/11
N2 - Mammalian angiotensin-converting enzyme (ACE) exists as two forms, somatic (sACE), controlling blood pressure via angiotensin II, and testicular (tACE), whose function is unknown. The former has two highly homologous N- and C-terminal Zn2+ metallopeptidase active sites, whereas the latter only has one, which is identical to the C-terminal domain of sACE. We have sequenced 2452 bases of a 3.1-kb mRNA whose predicted translation product shows 40% identity with mammalian testicular ACE, and 48% identity with an already identified Drosophila homologue of ACE (Ance). We have termed this gene Acer (Angiotensin converting enzyme-related). Acer mRNA is found in the developing dorsal vessel (heart) during embryogenesis. Phylogenetic analysis indicates that duplication of an ancestral ACE gene occurred in the lineage leading to the arthropods, independently of the duplication which gave rise to the two domain somatic ACE of mammals.
AB - Mammalian angiotensin-converting enzyme (ACE) exists as two forms, somatic (sACE), controlling blood pressure via angiotensin II, and testicular (tACE), whose function is unknown. The former has two highly homologous N- and C-terminal Zn2+ metallopeptidase active sites, whereas the latter only has one, which is identical to the C-terminal domain of sACE. We have sequenced 2452 bases of a 3.1-kb mRNA whose predicted translation product shows 40% identity with mammalian testicular ACE, and 48% identity with an already identified Drosophila homologue of ACE (Ance). We have termed this gene Acer (Angiotensin converting enzyme-related). Acer mRNA is found in the developing dorsal vessel (heart) during embryogenesis. Phylogenetic analysis indicates that duplication of an ancestral ACE gene occurred in the lineage leading to the arthropods, independently of the duplication which gave rise to the two domain somatic ACE of mammals.
KW - Dipeptidyl dipeptidase
KW - Metalloproteinase
KW - Dna sequencing
KW - Gene duplication
KW - Zn2+-Binding motif
KW - Heart
U2 - 10.1016/S0378-1119(96)00503-3
DO - 10.1016/S0378-1119(96)00503-3
M3 - Journal article
VL - 181
SP - 191
EP - 197
JO - Gene
JF - Gene
IS - 1-2
ER -