The activities of PEP carboxylase and the C4 acid decarboxylases are little changed by drought stress in three C4 grasses of different subtypes

Lancaster Environment Centre

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https://doi.org/10.1007/s11120-008-9329-7
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Keywords

C grasses, Drought stress, NAD-ME, NADP-ME, PEPC, PEPCK

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The activities of PEP carboxylase and the C4 acid decarboxylases are little changed by drought stress in three C4 grasses of different subtypes. / Carmo-Silva, Ana E.; Bernardes Da Silva, Anabela; Keys, Alfred J. et al.
In: Photosynthesis Research, Vol. 97, No. 3, 09.2008, p. 223-233.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Bibtex

@article{84876123a6524a7b91989856baf4f0ef,

title = "The activities of PEP carboxylase and the C4 acid decarboxylases are little changed by drought stress in three C4 grasses of different subtypes",

abstract = "The C4 photosynthetic pathway involves the assimilation of CO2 by phosphoenolpyruvate carboxylase (PEPC) and the subsequent decarboxylation of C4 acids. The enzymes of the CO2 concentrating mechanism could be affected under water deficit and limit C 4 photosynthesis. Three different C4 grasses were submitted to gradually induced drought stress conditions: Paspalum dilatatum (NADP-malic enzyme, NADP-ME), Cynodon dactylon (NAD-malic enzyme, NAD-ME) and Zoysia japonica (PEP carboxykinase, PEPCK). Moderate leaf dehydration affected the activity and regulation of PEPC in a similar manner in the three grasses but had species-specific effects on the C4 acid decarboxylases, NADP-ME, NAD-ME and PEPCK, although changes in the C4 enzyme activities were small. In all three species, the PEPC phosphorylation state, judged by the inhibitory effect of l-malate on PEPC activity, increased with water deficit and could promote increased assimilation of CO2 by the enzyme under stress conditions. Appreciable activity of PEPCK was observed in all three species suggesting that this enzyme may act as a supplementary decarboxylase to NADP-ME and NAD-ME in addition to its role in other metabolic pathways.",

keywords = "C grasses, Drought stress, NAD-ME, NADP-ME, PEPC, PEPCK",

author = "Carmo-Silva, {Ana E.} and {Bernardes Da Silva}, Anabela and Keys, {Alfred J.} and Parry, {Martin A. J.} and Arraba{\c c}a, {Maria C.}",

year = "2008",

month = sep,

doi = "10.1007/s11120-008-9329-7",

language = "English",

volume = "97",

pages = "223--233",

journal = "Photosynthesis Research",

issn = "0166-8595",

publisher = "Springer Netherlands",

number = "3",

}

RIS

TY - JOUR

T1 - The activities of PEP carboxylase and the C4 acid decarboxylases are little changed by drought stress in three C4 grasses of different subtypes

AU - Carmo-Silva, Ana E.

AU - Bernardes Da Silva, Anabela

AU - Keys, Alfred J.

AU - Parry, Martin A. J.

AU - Arrabaça, Maria C.

PY - 2008/9

Y1 - 2008/9

N2 - The C4 photosynthetic pathway involves the assimilation of CO2 by phosphoenolpyruvate carboxylase (PEPC) and the subsequent decarboxylation of C4 acids. The enzymes of the CO2 concentrating mechanism could be affected under water deficit and limit C 4 photosynthesis. Three different C4 grasses were submitted to gradually induced drought stress conditions: Paspalum dilatatum (NADP-malic enzyme, NADP-ME), Cynodon dactylon (NAD-malic enzyme, NAD-ME) and Zoysia japonica (PEP carboxykinase, PEPCK). Moderate leaf dehydration affected the activity and regulation of PEPC in a similar manner in the three grasses but had species-specific effects on the C4 acid decarboxylases, NADP-ME, NAD-ME and PEPCK, although changes in the C4 enzyme activities were small. In all three species, the PEPC phosphorylation state, judged by the inhibitory effect of l-malate on PEPC activity, increased with water deficit and could promote increased assimilation of CO2 by the enzyme under stress conditions. Appreciable activity of PEPCK was observed in all three species suggesting that this enzyme may act as a supplementary decarboxylase to NADP-ME and NAD-ME in addition to its role in other metabolic pathways.

AB - The C4 photosynthetic pathway involves the assimilation of CO2 by phosphoenolpyruvate carboxylase (PEPC) and the subsequent decarboxylation of C4 acids. The enzymes of the CO2 concentrating mechanism could be affected under water deficit and limit C 4 photosynthesis. Three different C4 grasses were submitted to gradually induced drought stress conditions: Paspalum dilatatum (NADP-malic enzyme, NADP-ME), Cynodon dactylon (NAD-malic enzyme, NAD-ME) and Zoysia japonica (PEP carboxykinase, PEPCK). Moderate leaf dehydration affected the activity and regulation of PEPC in a similar manner in the three grasses but had species-specific effects on the C4 acid decarboxylases, NADP-ME, NAD-ME and PEPCK, although changes in the C4 enzyme activities were small. In all three species, the PEPC phosphorylation state, judged by the inhibitory effect of l-malate on PEPC activity, increased with water deficit and could promote increased assimilation of CO2 by the enzyme under stress conditions. Appreciable activity of PEPCK was observed in all three species suggesting that this enzyme may act as a supplementary decarboxylase to NADP-ME and NAD-ME in addition to its role in other metabolic pathways.

KW - C grasses

KW - Drought stress

KW - NAD-ME

KW - NADP-ME

KW - PEPC

KW - PEPCK

U2 - 10.1007/s11120-008-9329-7

DO - 10.1007/s11120-008-9329-7

M3 - Journal article

C2 - 18629606

AN - SCOPUS:51749093961

VL - 97

SP - 223

EP - 233

JO - Photosynthesis Research

JF - Photosynthesis Research

SN - 0166-8595

IS - 3

ER -

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