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The Atr and Atm protein kinases associate with different sites along meiotically pairing chromosomes.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

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The Atr and Atm protein kinases associate with different sites along meiotically pairing chromosomes. / Keegan, K.S.; Holtzman, D.A.; Plug, A.W.; Christenson, E.R.; Brainerd, E.E.; Flaggs, G.; Bentley, N.J.; Taylor, Elaine M.; Meyn, M.S.; Moss, S.B.; Carr, A.M.; Ashley, T.; Hoekstra, M.F.

In: Genes and Development, Vol. 10, No. 19, 01.10.1996, p. 2423-2437.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Keegan, KS, Holtzman, DA, Plug, AW, Christenson, ER, Brainerd, EE, Flaggs, G, Bentley, NJ, Taylor, EM, Meyn, MS, Moss, SB, Carr, AM, Ashley, T & Hoekstra, MF 1996, 'The Atr and Atm protein kinases associate with different sites along meiotically pairing chromosomes.', Genes and Development, vol. 10, no. 19, pp. 2423-2437. https://doi.org/10.1101/gad.10.19.2423

APA

Keegan, K. S., Holtzman, D. A., Plug, A. W., Christenson, E. R., Brainerd, E. E., Flaggs, G., Bentley, N. J., Taylor, E. M., Meyn, M. S., Moss, S. B., Carr, A. M., Ashley, T., & Hoekstra, M. F. (1996). The Atr and Atm protein kinases associate with different sites along meiotically pairing chromosomes. Genes and Development, 10(19), 2423-2437. https://doi.org/10.1101/gad.10.19.2423

Vancouver

Keegan KS, Holtzman DA, Plug AW, Christenson ER, Brainerd EE, Flaggs G et al. The Atr and Atm protein kinases associate with different sites along meiotically pairing chromosomes. Genes and Development. 1996 Oct 1;10(19):2423-2437. https://doi.org/10.1101/gad.10.19.2423

Author

Keegan, K.S. ; Holtzman, D.A. ; Plug, A.W. ; Christenson, E.R. ; Brainerd, E.E. ; Flaggs, G. ; Bentley, N.J. ; Taylor, Elaine M. ; Meyn, M.S. ; Moss, S.B. ; Carr, A.M. ; Ashley, T. ; Hoekstra, M.F. / The Atr and Atm protein kinases associate with different sites along meiotically pairing chromosomes. In: Genes and Development. 1996 ; Vol. 10, No. 19. pp. 2423-2437.

Bibtex

@article{da650be6ad2b4e9a8f85462d06020f07,
title = "The Atr and Atm protein kinases associate with different sites along meiotically pairing chromosomes.",
abstract = "A number of cell-cycle checkpoint genes have been shown to play important roles in meiosis. We have characterized the human and mouse counterpart of the Schizosaccharomyces pombe Rad3 protein, named Atr (for ataxia-telangiectasia- and rad3-related), and the protein that is mutated in ataxia-telangiectasia, Atm. We demonstrate that ATR mRNA and protein are expressed in human and mouse testis. More detailed analysis of specific cells in seminiferous tubules shows localization of Atr to the nuclei of cells in the process of meiosis I. Using immunoprecipitation and immunoblot analysis, we show that Atr and Atm proteins are approximately 300 and 350 kD relative molecular mass, respectively, and further demonstrate that both proteins have associated protein kinase activity. Further, we demonstrate that Atr and Atm interact directly with meiotic chromosomes and show complementary localization patterns on synapsing chromosomes. Atr is found at sites along unpaired or asynapsed chromosomal axes, whereas Atm is found along synapsed chromosomal axes. This is the first demonstration of a nuclear association of Atr and Atm proteins with meiotic chromosomes and suggests a direct role for these proteins in recognizing and responding to DNA strand interruptions that occur during meiotic recombination.",
author = "K.S. Keegan and D.A. Holtzman and A.W. Plug and E.R. Christenson and E.E. Brainerd and G. Flaggs and N.J. Bentley and Taylor, {Elaine M.} and M.S. Meyn and S.B. Moss and A.M. Carr and T. Ashley and M.F. Hoekstra",
year = "1996",
month = oct,
day = "1",
doi = "10.1101/gad.10.19.2423",
language = "English",
volume = "10",
pages = "2423--2437",
journal = "Genes and Development",
issn = "0890-9369",
publisher = "Cold Spring Harbor Laboratory Press",
number = "19",

}

RIS

TY - JOUR

T1 - The Atr and Atm protein kinases associate with different sites along meiotically pairing chromosomes.

AU - Keegan, K.S.

AU - Holtzman, D.A.

AU - Plug, A.W.

AU - Christenson, E.R.

AU - Brainerd, E.E.

AU - Flaggs, G.

AU - Bentley, N.J.

AU - Taylor, Elaine M.

AU - Meyn, M.S.

AU - Moss, S.B.

AU - Carr, A.M.

AU - Ashley, T.

AU - Hoekstra, M.F.

PY - 1996/10/1

Y1 - 1996/10/1

N2 - A number of cell-cycle checkpoint genes have been shown to play important roles in meiosis. We have characterized the human and mouse counterpart of the Schizosaccharomyces pombe Rad3 protein, named Atr (for ataxia-telangiectasia- and rad3-related), and the protein that is mutated in ataxia-telangiectasia, Atm. We demonstrate that ATR mRNA and protein are expressed in human and mouse testis. More detailed analysis of specific cells in seminiferous tubules shows localization of Atr to the nuclei of cells in the process of meiosis I. Using immunoprecipitation and immunoblot analysis, we show that Atr and Atm proteins are approximately 300 and 350 kD relative molecular mass, respectively, and further demonstrate that both proteins have associated protein kinase activity. Further, we demonstrate that Atr and Atm interact directly with meiotic chromosomes and show complementary localization patterns on synapsing chromosomes. Atr is found at sites along unpaired or asynapsed chromosomal axes, whereas Atm is found along synapsed chromosomal axes. This is the first demonstration of a nuclear association of Atr and Atm proteins with meiotic chromosomes and suggests a direct role for these proteins in recognizing and responding to DNA strand interruptions that occur during meiotic recombination.

AB - A number of cell-cycle checkpoint genes have been shown to play important roles in meiosis. We have characterized the human and mouse counterpart of the Schizosaccharomyces pombe Rad3 protein, named Atr (for ataxia-telangiectasia- and rad3-related), and the protein that is mutated in ataxia-telangiectasia, Atm. We demonstrate that ATR mRNA and protein are expressed in human and mouse testis. More detailed analysis of specific cells in seminiferous tubules shows localization of Atr to the nuclei of cells in the process of meiosis I. Using immunoprecipitation and immunoblot analysis, we show that Atr and Atm proteins are approximately 300 and 350 kD relative molecular mass, respectively, and further demonstrate that both proteins have associated protein kinase activity. Further, we demonstrate that Atr and Atm interact directly with meiotic chromosomes and show complementary localization patterns on synapsing chromosomes. Atr is found at sites along unpaired or asynapsed chromosomal axes, whereas Atm is found along synapsed chromosomal axes. This is the first demonstration of a nuclear association of Atr and Atm proteins with meiotic chromosomes and suggests a direct role for these proteins in recognizing and responding to DNA strand interruptions that occur during meiotic recombination.

U2 - 10.1101/gad.10.19.2423

DO - 10.1101/gad.10.19.2423

M3 - Journal article

VL - 10

SP - 2423

EP - 2437

JO - Genes and Development

JF - Genes and Development

SN - 0890-9369

IS - 19

ER -