Research output: Contribution to Journal/Magazine › Journal article
Research output: Contribution to Journal/Magazine › Journal article
}
TY - JOUR
T1 - The calcium dependence of electrical and mechanical responses of the odontophore protractor muscle of Busycon canaliculatum. A sucrose-gap study of calcium antagonist action.
AU - Huddart, H.
AU - Nelson, I. D.
AU - Brooks, D. D.
AU - Hill, R. B.
PY - 1992/6
Y1 - 1992/6
N2 - 1. 1. Exposures for 30 min to Ca-free salines irreversibly inhibited responses of the odontophore protractor (OP) muscles of Busycon canaliculatum to ACh and high K+ salines. Under continuous field stimulation, Ca-free salines extinguished the responses within 3 min and recovery on Ca readmission was only partial. 2. 2. Nifedipine converted normally smooth ACh-induced depolarizations into oscillatory events generating small twitch-like contractions. 3. 3. Nifedipine converted normally tonic high K+ responses into depolarizations with fast spike-like action potentials generating fast twitches. The low K+induced action potentials and fast twitches of this muscle were greatly enhanced by nifedipine. Nifedipine induced rapid transient inward current pulses accompanying this twitch activity. 4. 4. Co2+, Cd2+ and Gd3+ all inhibited the ACh and K+ responses of the OP muscle but Gd3+ was the most consistent and potent inhibitor of these responses. 5. 5. Gd3+ did not affect K+- or ACh-induced depolarization levels but eliminated spike-like action potentials and the twitches they generated. 6. 6. When the muscle membranes were depolarized with increasing K+ subsequent ACh responses were inhibited and eventually eliminated at about −41 mV, indicating that the ACh receptor here showed voltage inactivation. 7. 7. Although dependent upon an influx of[Ca]0 for CIRC to effect EC coupling, this Ca appears to enter the cells by a mechanism unlike that of mammalian smooth muscle. The cellular Ca pool appears to be independently but not synergistically accessed by ACh and K+ in the induction of contractile responses.
AB - 1. 1. Exposures for 30 min to Ca-free salines irreversibly inhibited responses of the odontophore protractor (OP) muscles of Busycon canaliculatum to ACh and high K+ salines. Under continuous field stimulation, Ca-free salines extinguished the responses within 3 min and recovery on Ca readmission was only partial. 2. 2. Nifedipine converted normally smooth ACh-induced depolarizations into oscillatory events generating small twitch-like contractions. 3. 3. Nifedipine converted normally tonic high K+ responses into depolarizations with fast spike-like action potentials generating fast twitches. The low K+induced action potentials and fast twitches of this muscle were greatly enhanced by nifedipine. Nifedipine induced rapid transient inward current pulses accompanying this twitch activity. 4. 4. Co2+, Cd2+ and Gd3+ all inhibited the ACh and K+ responses of the OP muscle but Gd3+ was the most consistent and potent inhibitor of these responses. 5. 5. Gd3+ did not affect K+- or ACh-induced depolarization levels but eliminated spike-like action potentials and the twitches they generated. 6. 6. When the muscle membranes were depolarized with increasing K+ subsequent ACh responses were inhibited and eventually eliminated at about −41 mV, indicating that the ACh receptor here showed voltage inactivation. 7. 7. Although dependent upon an influx of[Ca]0 for CIRC to effect EC coupling, this Ca appears to enter the cells by a mechanism unlike that of mammalian smooth muscle. The cellular Ca pool appears to be independently but not synergistically accessed by ACh and K+ in the induction of contractile responses.
U2 - 10.1016/0300-9629(92)90139-H
DO - 10.1016/0300-9629(92)90139-H
M3 - Journal article
VL - 102
SP - 299
EP - 305
JO - Comparative Biochemistry and Physiology Part A: Physiology
JF - Comparative Biochemistry and Physiology Part A: Physiology
IS - 2
ER -