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The challenge of applying Raman spectroscopy to monitor recombinant antibody production

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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The challenge of applying Raman spectroscopy to monitor recombinant antibody production. / Ashton, Lorna; Xu, Yun; Brewster, Victoria L. et al.
In: Analyst, Vol. 138, No. 22, 26.09.2013, p. 6977-6985.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Ashton, L, Xu, Y, Brewster, VL, Cowcher, DP, Sellick, CA, Dickson, AJ, Stephens, GM & Goodacre, R 2013, 'The challenge of applying Raman spectroscopy to monitor recombinant antibody production', Analyst, vol. 138, no. 22, pp. 6977-6985. https://doi.org/10.1039/c3an01341c

APA

Ashton, L., Xu, Y., Brewster, V. L., Cowcher, D. P., Sellick, C. A., Dickson, A. J., Stephens, G. M., & Goodacre, R. (2013). The challenge of applying Raman spectroscopy to monitor recombinant antibody production. Analyst, 138(22), 6977-6985. https://doi.org/10.1039/c3an01341c

Vancouver

Ashton L, Xu Y, Brewster VL, Cowcher DP, Sellick CA, Dickson AJ et al. The challenge of applying Raman spectroscopy to monitor recombinant antibody production. Analyst. 2013 Sept 26;138(22):6977-6985. doi: 10.1039/c3an01341c

Author

Ashton, Lorna ; Xu, Yun ; Brewster, Victoria L. et al. / The challenge of applying Raman spectroscopy to monitor recombinant antibody production. In: Analyst. 2013 ; Vol. 138, No. 22. pp. 6977-6985.

Bibtex

@article{7e75cd4514014f95b183e2875108b06f,
title = "The challenge of applying Raman spectroscopy to monitor recombinant antibody production",
abstract = "UV resonance Raman (UVRR) spectroscopy combined with chemometric techniques was investigated as a physiochemical tool for monitoring secreted recombinant antibody production in cultures of Chinese hamster ovary (CHO) cells. Due to the enhanced selectivity of the UVRR, spectral variations arising from protein, small molecule substrates, and nucleic acid medium components could be measured simultaneously and we have successfully determined antibody titre. Medium samples were taken during culture of three CHO cell lines: two antibody-producing cell lines and a non-producing cell line, and analysed by UVRR spectroscopy using an excitation laser of 244 nm. Principal component analysis (PCA) was applied to the spectral sets and showed a linear trend over time for the antibody-producing cell lines that was not observed in the non-producing cell line. Partial least squares regression (PLSR) was used to predict antibody titres, glucose utilization and lactate accumulation, and compared very favourably with gold standard data acquired with the much slower techniques of ELISA and liquid chromatography. Further analysis of the UVRR spectral sets using two-dimensional correlation moving windows also revealed that spectral variations due to protein and nucleic acid concentrations in the medium during cell culture varied between each of the three cell lines investigated.",
keywords = "CHEMOMETRICS, CHEMISTRY, NM",
author = "Lorna Ashton and Yun Xu and Brewster, {Victoria L.} and Cowcher, {David P.} and Sellick, {Christopher A.} and Dickson, {Alan J.} and Stephens, {Gill M.} and Royston Goodacre",
year = "2013",
month = sep,
day = "26",
doi = "10.1039/c3an01341c",
language = "English",
volume = "138",
pages = "6977--6985",
journal = "Analyst",
issn = "0003-2654",
publisher = "Royal Society of Chemistry",
number = "22",

}

RIS

TY - JOUR

T1 - The challenge of applying Raman spectroscopy to monitor recombinant antibody production

AU - Ashton, Lorna

AU - Xu, Yun

AU - Brewster, Victoria L.

AU - Cowcher, David P.

AU - Sellick, Christopher A.

AU - Dickson, Alan J.

AU - Stephens, Gill M.

AU - Goodacre, Royston

PY - 2013/9/26

Y1 - 2013/9/26

N2 - UV resonance Raman (UVRR) spectroscopy combined with chemometric techniques was investigated as a physiochemical tool for monitoring secreted recombinant antibody production in cultures of Chinese hamster ovary (CHO) cells. Due to the enhanced selectivity of the UVRR, spectral variations arising from protein, small molecule substrates, and nucleic acid medium components could be measured simultaneously and we have successfully determined antibody titre. Medium samples were taken during culture of three CHO cell lines: two antibody-producing cell lines and a non-producing cell line, and analysed by UVRR spectroscopy using an excitation laser of 244 nm. Principal component analysis (PCA) was applied to the spectral sets and showed a linear trend over time for the antibody-producing cell lines that was not observed in the non-producing cell line. Partial least squares regression (PLSR) was used to predict antibody titres, glucose utilization and lactate accumulation, and compared very favourably with gold standard data acquired with the much slower techniques of ELISA and liquid chromatography. Further analysis of the UVRR spectral sets using two-dimensional correlation moving windows also revealed that spectral variations due to protein and nucleic acid concentrations in the medium during cell culture varied between each of the three cell lines investigated.

AB - UV resonance Raman (UVRR) spectroscopy combined with chemometric techniques was investigated as a physiochemical tool for monitoring secreted recombinant antibody production in cultures of Chinese hamster ovary (CHO) cells. Due to the enhanced selectivity of the UVRR, spectral variations arising from protein, small molecule substrates, and nucleic acid medium components could be measured simultaneously and we have successfully determined antibody titre. Medium samples were taken during culture of three CHO cell lines: two antibody-producing cell lines and a non-producing cell line, and analysed by UVRR spectroscopy using an excitation laser of 244 nm. Principal component analysis (PCA) was applied to the spectral sets and showed a linear trend over time for the antibody-producing cell lines that was not observed in the non-producing cell line. Partial least squares regression (PLSR) was used to predict antibody titres, glucose utilization and lactate accumulation, and compared very favourably with gold standard data acquired with the much slower techniques of ELISA and liquid chromatography. Further analysis of the UVRR spectral sets using two-dimensional correlation moving windows also revealed that spectral variations due to protein and nucleic acid concentrations in the medium during cell culture varied between each of the three cell lines investigated.

KW - CHEMOMETRICS

KW - CHEMISTRY

KW - NM

U2 - 10.1039/c3an01341c

DO - 10.1039/c3an01341c

M3 - Journal article

VL - 138

SP - 6977

EP - 6985

JO - Analyst

JF - Analyst

SN - 0003-2654

IS - 22

ER -