Home > Research > Publications & Outputs > The dynamics and orientation of a lipophilic dr...

Text available via DOI:

View graph of relations

The dynamics and orientation of a lipophilic drug within model membranes determined by 13C solid-state NMR

Research output: Contribution to journalJournal articlepeer-review

Published
<mark>Journal publication date</mark>7/01/2008
<mark>Journal</mark>Physical Chemistry Chemical Physics
Issue number1
Volume10
Number of pages8
Pages (from-to)178-85
Publication StatusPublished
<mark>Original language</mark>English

Abstract

Methods for determining how a drug interacts with cellular membranes at the molecular level can give valuable insight into the mode of action of the drug and its absorption, distribution and metabolism profile. A procedure is described here to determine the orientation and location of the lipophilic drug trifluoperazine (TFP) intercalated into dimyristoylphosphatidylcholine (DMPC) bilayers, by using a novel combination of high-resolution solid-state nuclear magnetic resonance (SSNMR) methods to observe signals from (13)C within the drug at natural abundance. SSNMR measurements of (1)H-(13)C dipolar couplings for TFP and selective broadening of (13)C NMR peaks by paramagnetic Mn(2+) together suggest a model for the location, orientation and dynamics of the drug within lipid bilayers that offers an explanation for the lysoprotective effect of the drug at low concentrations. The experiments described are straightforward to implement and can be used for the routine analysis of drug-membrane interactions to provide useful information for drug design and structure refinement.