Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - The effects of nested miRNAs and their host genes on immune defense against Bacillus thuringiensis infection in Caenorhabditis elegans
AU - Zárate-Potes, Alejandra
AU - Yang, Wentao
AU - Andresen, Bentje
AU - Nakad, Rania
AU - Haase, Daniela
AU - Rosenstiel, Philip
AU - Dierking, Katja
AU - Schulenburg, Hinrich
PY - 2021/10/31
Y1 - 2021/10/31
N2 - microRNAs (miRNAs) are small non-coding RNA-molecules that influence translation by binding to the target gene mRNA. Many miRNAs are found in nested arrangements within larger protein-coding host genes. miRNAs and host genes in a nested arrangement are often transcribed simultaneously, which may indicate that both have similar functions. miRNAs have been implicated in regulating defense responses against pathogen infection in C. elegans and in mammals. Here, we asked if miRNAs in nested arrangements and their host genes are involved in the C. elegans response against infection with Bacillus thuringiensis (Bt). We performed miRNA sequencing and subsequently focused on four nested miRNA-host gene arrangements for a functional genetic analysis. We identified mir-58.1 and mir-2 as negative regulators of C. elegans resistance to Bt infection. However, we did not find any miRNA/host gene pair in which both contribute to defense against Bt.
AB - microRNAs (miRNAs) are small non-coding RNA-molecules that influence translation by binding to the target gene mRNA. Many miRNAs are found in nested arrangements within larger protein-coding host genes. miRNAs and host genes in a nested arrangement are often transcribed simultaneously, which may indicate that both have similar functions. miRNAs have been implicated in regulating defense responses against pathogen infection in C. elegans and in mammals. Here, we asked if miRNAs in nested arrangements and their host genes are involved in the C. elegans response against infection with Bacillus thuringiensis (Bt). We performed miRNA sequencing and subsequently focused on four nested miRNA-host gene arrangements for a functional genetic analysis. We identified mir-58.1 and mir-2 as negative regulators of C. elegans resistance to Bt infection. However, we did not find any miRNA/host gene pair in which both contribute to defense against Bt.
KW - Animals
KW - Bacillus thuringiensis/physiology
KW - Caenorhabditis elegans/immunology
KW - Caenorhabditis elegans Proteins/genetics
KW - Disease Resistance
KW - Gram-Positive Bacterial Infections/immunology
KW - Host-Pathogen Interactions
KW - Immunity, Innate
KW - MicroRNAs/genetics
KW - Sequence Analysis, RNA
U2 - 10.1016/j.dci.2021.104144
DO - 10.1016/j.dci.2021.104144
M3 - Journal article
C2 - 34051205
VL - 123
JO - Developmental and comparative immunology
JF - Developmental and comparative immunology
SN - 0145-305X
M1 - 104144
ER -