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    Rights statement: https://www.cambridge.org/core/journals/parasitology/article/abs/efficacy-of-recombinant-protein-lbk39-for-the-diagnosis-of-leishmaniosis-in-dogs/B5EA8AA4B4B2F221F59944689032621F The final, definitive version of this article has been published in the Journal, Parasitology, 148, 3, pp 302-310 2020, © 2020 Cambridge University Press.

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The efficacy of recombinant protein lbk39 for the diagnosis of leishmaniosis in dogs

Research output: Contribution to Journal/MagazineJournal articlepeer-review

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The efficacy of recombinant protein lbk39 for the diagnosis of leishmaniosis in dogs. / Pereira, J.C.; Dos Santos Sousa, P.; De Souza, L.M.B.; Pasquali, A.K.S.; Bates, M.; Bates, P.; Thomaz Soccol, V.

In: Parasitology, Vol. 148, No. 3, 01.03.2021, p. 302-310.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Pereira, JC, Dos Santos Sousa, P, De Souza, LMB, Pasquali, AKS, Bates, M, Bates, P & Thomaz Soccol, V 2021, 'The efficacy of recombinant protein lbk39 for the diagnosis of leishmaniosis in dogs', Parasitology, vol. 148, no. 3, pp. 302-310. https://doi.org/10.1017/S0031182020001997

APA

Pereira, J. C., Dos Santos Sousa, P., De Souza, L. M. B., Pasquali, A. K. S., Bates, M., Bates, P., & Thomaz Soccol, V. (2021). The efficacy of recombinant protein lbk39 for the diagnosis of leishmaniosis in dogs. Parasitology, 148(3), 302-310. https://doi.org/10.1017/S0031182020001997

Vancouver

Pereira JC, Dos Santos Sousa P, De Souza LMB, Pasquali AKS, Bates M, Bates P et al. The efficacy of recombinant protein lbk39 for the diagnosis of leishmaniosis in dogs. Parasitology. 2021 Mar 1;148(3):302-310. https://doi.org/10.1017/S0031182020001997

Author

Pereira, J.C. ; Dos Santos Sousa, P. ; De Souza, L.M.B. ; Pasquali, A.K.S. ; Bates, M. ; Bates, P. ; Thomaz Soccol, V. / The efficacy of recombinant protein lbk39 for the diagnosis of leishmaniosis in dogs. In: Parasitology. 2021 ; Vol. 148, No. 3. pp. 302-310.

Bibtex

@article{f1060e18210d46449b92323d5be4a4dc,
title = "The efficacy of recombinant protein lbk39 for the diagnosis of leishmaniosis in dogs",
abstract = "Visceral leishmaniosis is one of the most important zoonotic diseases on the planet and dogs are the main reservoir of canine visceral leishmaniosis (CVL) in endemic areas. They play an important role in human infection because in dogs the disease appears long time after infection, and they can move uncontrollably, contributing to disperse the parasite. To take the decision to treat the animals or for euthanasia, in an elimination programme, in order to reduce the parasitic load, it is necessary to diagnose correctly, having more effective tools. Our group has developed a new recombinant antigen-based kinesin-related gene of Leishmania braziliensis (Lbk39), which shows 59% amino acid identity to the L. infantum homologue. The Lbk39 gene was synthesized, inserted into the pLEXSY-sat2 vector and transfected into L. tarentolae cells by electroporation. The recombinant protein was secreted in the culture with a C-terminal histidine marker, purified, generating a product at 337.68 μg mL-1. A total of 152 sera from dog's endemic and non-endemic areas were used, being 78 positives and 75 negatives. The antigen Lbk39 showed 100% sensitivity and 96.1% specificity. We compared this antigen with other antigens such as total extract of the parasite, TRDPP, and our data indicate that Lbk39 has potential application in the diagnosis of CVL through antibody detection. Copyright {\textcopyright} The Author(s), 2020. Published by Cambridge University Press.",
keywords = "Canis familiaris, dogs, ELISA, recombinant antigen, visceral leishmaniosis",
author = "J.C. Pereira and {Dos Santos Sousa}, P. and {De Souza}, L.M.B. and A.K.S. Pasquali and M. Bates and P. Bates and {Thomaz Soccol}, V.",
note = "https://www.cambridge.org/core/journals/parasitology/article/abs/efficacy-of-recombinant-protein-lbk39-for-the-diagnosis-of-leishmaniosis-in-dogs/B5EA8AA4B4B2F221F59944689032621F The final, definitive version of this article has been published in the Journal, Parasitology, 148, 3, pp 302-310 2020, {\textcopyright} 2020 Cambridge University Press. ",
year = "2021",
month = mar,
day = "1",
doi = "10.1017/S0031182020001997",
language = "English",
volume = "148",
pages = "302--310",
journal = "Parasitology",
issn = "0031-1820",
publisher = "Cambridge University Press",
number = "3",

}

RIS

TY - JOUR

T1 - The efficacy of recombinant protein lbk39 for the diagnosis of leishmaniosis in dogs

AU - Pereira, J.C.

AU - Dos Santos Sousa, P.

AU - De Souza, L.M.B.

AU - Pasquali, A.K.S.

AU - Bates, M.

AU - Bates, P.

AU - Thomaz Soccol, V.

N1 - https://www.cambridge.org/core/journals/parasitology/article/abs/efficacy-of-recombinant-protein-lbk39-for-the-diagnosis-of-leishmaniosis-in-dogs/B5EA8AA4B4B2F221F59944689032621F The final, definitive version of this article has been published in the Journal, Parasitology, 148, 3, pp 302-310 2020, © 2020 Cambridge University Press.

PY - 2021/3/1

Y1 - 2021/3/1

N2 - Visceral leishmaniosis is one of the most important zoonotic diseases on the planet and dogs are the main reservoir of canine visceral leishmaniosis (CVL) in endemic areas. They play an important role in human infection because in dogs the disease appears long time after infection, and they can move uncontrollably, contributing to disperse the parasite. To take the decision to treat the animals or for euthanasia, in an elimination programme, in order to reduce the parasitic load, it is necessary to diagnose correctly, having more effective tools. Our group has developed a new recombinant antigen-based kinesin-related gene of Leishmania braziliensis (Lbk39), which shows 59% amino acid identity to the L. infantum homologue. The Lbk39 gene was synthesized, inserted into the pLEXSY-sat2 vector and transfected into L. tarentolae cells by electroporation. The recombinant protein was secreted in the culture with a C-terminal histidine marker, purified, generating a product at 337.68 μg mL-1. A total of 152 sera from dog's endemic and non-endemic areas were used, being 78 positives and 75 negatives. The antigen Lbk39 showed 100% sensitivity and 96.1% specificity. We compared this antigen with other antigens such as total extract of the parasite, TRDPP, and our data indicate that Lbk39 has potential application in the diagnosis of CVL through antibody detection. Copyright © The Author(s), 2020. Published by Cambridge University Press.

AB - Visceral leishmaniosis is one of the most important zoonotic diseases on the planet and dogs are the main reservoir of canine visceral leishmaniosis (CVL) in endemic areas. They play an important role in human infection because in dogs the disease appears long time after infection, and they can move uncontrollably, contributing to disperse the parasite. To take the decision to treat the animals or for euthanasia, in an elimination programme, in order to reduce the parasitic load, it is necessary to diagnose correctly, having more effective tools. Our group has developed a new recombinant antigen-based kinesin-related gene of Leishmania braziliensis (Lbk39), which shows 59% amino acid identity to the L. infantum homologue. The Lbk39 gene was synthesized, inserted into the pLEXSY-sat2 vector and transfected into L. tarentolae cells by electroporation. The recombinant protein was secreted in the culture with a C-terminal histidine marker, purified, generating a product at 337.68 μg mL-1. A total of 152 sera from dog's endemic and non-endemic areas were used, being 78 positives and 75 negatives. The antigen Lbk39 showed 100% sensitivity and 96.1% specificity. We compared this antigen with other antigens such as total extract of the parasite, TRDPP, and our data indicate that Lbk39 has potential application in the diagnosis of CVL through antibody detection. Copyright © The Author(s), 2020. Published by Cambridge University Press.

KW - Canis familiaris

KW - dogs

KW - ELISA

KW - recombinant antigen

KW - visceral leishmaniosis

U2 - 10.1017/S0031182020001997

DO - 10.1017/S0031182020001997

M3 - Journal article

VL - 148

SP - 302

EP - 310

JO - Parasitology

JF - Parasitology

SN - 0031-1820

IS - 3

ER -