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The eggshell membrane: A potential biomaterial for corneal wound healing

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The eggshell membrane: A potential biomaterial for corneal wound healing. / Mensah, R.A.; Jo, S.B.; Kim, H. et al.
In: JOURNAL OF BIOMATERIALS APPLICATIONS, Vol. 36, No. 5, 01.11.2021, p. 912-929.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Mensah, RA, Jo, SB, Kim, H, Park, S-M, Patel, KD, Cho, KJ, Cook, MT, Kirton, SB, Hutter, V, Sidney, LE, Alves-Lima, D, Lin, H, Lee, J-H, Kim, H & Chau, DYS 2021, 'The eggshell membrane: A potential biomaterial for corneal wound healing', JOURNAL OF BIOMATERIALS APPLICATIONS, vol. 36, no. 5, pp. 912-929. https://doi.org/10.1177/08853282211024040

APA

Mensah, R. A., Jo, S. B., Kim, H., Park, S-M., Patel, K. D., Cho, K. J., Cook, M. T., Kirton, S. B., Hutter, V., Sidney, L. E., Alves-Lima, D., Lin, H., Lee, J-H., Kim, H., & Chau, D. Y. S. (2021). The eggshell membrane: A potential biomaterial for corneal wound healing. JOURNAL OF BIOMATERIALS APPLICATIONS, 36(5), 912-929. https://doi.org/10.1177/08853282211024040

Vancouver

Mensah RA, Jo SB, Kim H, Park S-M, Patel KD, Cho KJ et al. The eggshell membrane: A potential biomaterial for corneal wound healing. JOURNAL OF BIOMATERIALS APPLICATIONS. 2021 Nov 1;36(5):912-929. Epub 2021 Jun 18. doi: 10.1177/08853282211024040

Author

Mensah, R.A. ; Jo, S.B. ; Kim, H. et al. / The eggshell membrane : A potential biomaterial for corneal wound healing. In: JOURNAL OF BIOMATERIALS APPLICATIONS. 2021 ; Vol. 36, No. 5. pp. 912-929.

Bibtex

@article{a03d362708be4e82885556ec06944818,
title = "The eggshell membrane: A potential biomaterial for corneal wound healing",
abstract = "The eggshell membrane (ESM) is an abundant resource with innate complex structure and composition provided by nature. With at least 60 million tonnes of hen eggs produced globally per annum, utilisation of this waste resource is highly attractive in positively impacting sustainability worldwide. Given the morphology and mechanical properties of this membrane, it has great potential as a biomaterials for wound dressing. However, to date, no studies have demonstrated nor reported this application. As such, the objective of this investigation was to identify and optimise a reproducible extraction protocol of the ESM and to assess the physical, chemical, mechanical and biological properties of the substrate with a view to use as a wound dressing. ESM samples were isolated by either manual peeling (ESM-strip) or via extraction using acetic acid [ESM-A0.5] or ethylenediaminetetraacetic acid, EDTA [ESM-E0.9]. Energy dispersive X-ray spectroscopy (EDS) confirmed that there were no traces of calcium residues from the extraction process. Fourier transform infrared (FTIR) spectroscopy revealed that the extraction method (acetic acid and EDTA) did not alter the chemical structures of the ESM and also clarified the composition of the fibrous proteins of the ESM. Scanning electron microscopy (SEM) analyses revealed a three-layer composite structure of the ESM: an inner layer as continuous, dense and non-fibrous (limiting membrane), a middle layer with a network of fibres (inner shell membrane) and the outer layer (outer shell membrane) of larger fibres. Material properties including optical transparency, porosity, fluid absorption/uptake, thermal stability, mechanical profiling of the ESM samples were performed and demonstrated suitable profiles for translational applications. Biological in vitro studies using SV40 immortalised corneal epithelial cells (ihCEC) and corneal mesenchymal stromal cells (C-MSC) demonstrated excellent biocompatibility. Taken together, these results document the development of a novel sustainable biomaterial that may be used for ophthalmic wounds and/or other biomedical therapies. ",
keywords = "biomimetic, cell culture, ECM, membrane, tissue engineering, wound dressing, Acetic acid, Biocompatibility, Biomechanics, Composite structures, Energy dispersive spectroscopy, Extraction, Fourier transform infrared spectroscopy, Membranes, Morphology, Network layers, Optical properties, pH, Scanning electron microscopy, Structure (composition), Tissue regeneration, Biological properties, Corneal epithelial cells, Energy dispersive X ray spectroscopy, Extraction protocols, Mechanical profiling, Mesenchymal stromal cells, Morphology and mechanical properties, Optical transparency, Ethylenediaminetetraacetic acid",
author = "R.A. Mensah and S.B. Jo and H. Kim and S.-M. Park and K.D. Patel and K.J. Cho and M.T. Cook and S.B. Kirton and V. Hutter and L.E. Sidney and D. Alves-Lima and H. Lin and J.-H. Lee and H. Kim and D.Y.S. Chau",
year = "2021",
month = nov,
day = "1",
doi = "10.1177/08853282211024040",
language = "English",
volume = "36",
pages = "912--929",
journal = "JOURNAL OF BIOMATERIALS APPLICATIONS",
issn = "0885-3282",
publisher = "SAGE Publications Ltd",
number = "5",

}

RIS

TY - JOUR

T1 - The eggshell membrane

T2 - A potential biomaterial for corneal wound healing

AU - Mensah, R.A.

AU - Jo, S.B.

AU - Kim, H.

AU - Park, S.-M.

AU - Patel, K.D.

AU - Cho, K.J.

AU - Cook, M.T.

AU - Kirton, S.B.

AU - Hutter, V.

AU - Sidney, L.E.

AU - Alves-Lima, D.

AU - Lin, H.

AU - Lee, J.-H.

AU - Kim, H.

AU - Chau, D.Y.S.

PY - 2021/11/1

Y1 - 2021/11/1

N2 - The eggshell membrane (ESM) is an abundant resource with innate complex structure and composition provided by nature. With at least 60 million tonnes of hen eggs produced globally per annum, utilisation of this waste resource is highly attractive in positively impacting sustainability worldwide. Given the morphology and mechanical properties of this membrane, it has great potential as a biomaterials for wound dressing. However, to date, no studies have demonstrated nor reported this application. As such, the objective of this investigation was to identify and optimise a reproducible extraction protocol of the ESM and to assess the physical, chemical, mechanical and biological properties of the substrate with a view to use as a wound dressing. ESM samples were isolated by either manual peeling (ESM-strip) or via extraction using acetic acid [ESM-A0.5] or ethylenediaminetetraacetic acid, EDTA [ESM-E0.9]. Energy dispersive X-ray spectroscopy (EDS) confirmed that there were no traces of calcium residues from the extraction process. Fourier transform infrared (FTIR) spectroscopy revealed that the extraction method (acetic acid and EDTA) did not alter the chemical structures of the ESM and also clarified the composition of the fibrous proteins of the ESM. Scanning electron microscopy (SEM) analyses revealed a three-layer composite structure of the ESM: an inner layer as continuous, dense and non-fibrous (limiting membrane), a middle layer with a network of fibres (inner shell membrane) and the outer layer (outer shell membrane) of larger fibres. Material properties including optical transparency, porosity, fluid absorption/uptake, thermal stability, mechanical profiling of the ESM samples were performed and demonstrated suitable profiles for translational applications. Biological in vitro studies using SV40 immortalised corneal epithelial cells (ihCEC) and corneal mesenchymal stromal cells (C-MSC) demonstrated excellent biocompatibility. Taken together, these results document the development of a novel sustainable biomaterial that may be used for ophthalmic wounds and/or other biomedical therapies.

AB - The eggshell membrane (ESM) is an abundant resource with innate complex structure and composition provided by nature. With at least 60 million tonnes of hen eggs produced globally per annum, utilisation of this waste resource is highly attractive in positively impacting sustainability worldwide. Given the morphology and mechanical properties of this membrane, it has great potential as a biomaterials for wound dressing. However, to date, no studies have demonstrated nor reported this application. As such, the objective of this investigation was to identify and optimise a reproducible extraction protocol of the ESM and to assess the physical, chemical, mechanical and biological properties of the substrate with a view to use as a wound dressing. ESM samples were isolated by either manual peeling (ESM-strip) or via extraction using acetic acid [ESM-A0.5] or ethylenediaminetetraacetic acid, EDTA [ESM-E0.9]. Energy dispersive X-ray spectroscopy (EDS) confirmed that there were no traces of calcium residues from the extraction process. Fourier transform infrared (FTIR) spectroscopy revealed that the extraction method (acetic acid and EDTA) did not alter the chemical structures of the ESM and also clarified the composition of the fibrous proteins of the ESM. Scanning electron microscopy (SEM) analyses revealed a three-layer composite structure of the ESM: an inner layer as continuous, dense and non-fibrous (limiting membrane), a middle layer with a network of fibres (inner shell membrane) and the outer layer (outer shell membrane) of larger fibres. Material properties including optical transparency, porosity, fluid absorption/uptake, thermal stability, mechanical profiling of the ESM samples were performed and demonstrated suitable profiles for translational applications. Biological in vitro studies using SV40 immortalised corneal epithelial cells (ihCEC) and corneal mesenchymal stromal cells (C-MSC) demonstrated excellent biocompatibility. Taken together, these results document the development of a novel sustainable biomaterial that may be used for ophthalmic wounds and/or other biomedical therapies.

KW - biomimetic

KW - cell culture

KW - ECM

KW - membrane

KW - tissue engineering

KW - wound dressing

KW - Acetic acid

KW - Biocompatibility

KW - Biomechanics

KW - Composite structures

KW - Energy dispersive spectroscopy

KW - Extraction

KW - Fourier transform infrared spectroscopy

KW - Membranes

KW - Morphology

KW - Network layers

KW - Optical properties

KW - pH

KW - Scanning electron microscopy

KW - Structure (composition)

KW - Tissue regeneration

KW - Biological properties

KW - Corneal epithelial cells

KW - Energy dispersive X ray spectroscopy

KW - Extraction protocols

KW - Mechanical profiling

KW - Mesenchymal stromal cells

KW - Morphology and mechanical properties

KW - Optical transparency

KW - Ethylenediaminetetraacetic acid

U2 - 10.1177/08853282211024040

DO - 10.1177/08853282211024040

M3 - Journal article

VL - 36

SP - 912

EP - 929

JO - JOURNAL OF BIOMATERIALS APPLICATIONS

JF - JOURNAL OF BIOMATERIALS APPLICATIONS

SN - 0885-3282

IS - 5

ER -