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The Human Skin Barrier Is Organized as Stacked Bilayers of Fully Extended Ceramides with Cholesterol Molecules Associated with the Ceramide Sphingoid Moiety

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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The Human Skin Barrier Is Organized as Stacked Bilayers of Fully Extended Ceramides with Cholesterol Molecules Associated with the Ceramide Sphingoid Moiety. / Iwai, Ichiro; Han, HongMei; den Hollander, Lianne et al.

In: Journal of Investigative Dermatology, Vol. 132, No. 9, 09.2012, p. 2215-2225.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Iwai, I, Han, H, den Hollander, L, Svensson, S, Oefverstedt, L-G, Anwar, J, Brewer, J, Bloksgaard, M, Laloeuf, A, Nosek, D, Masich, S, Bagatolli, LA, Skoglund, U & Norlen, L 2012, 'The Human Skin Barrier Is Organized as Stacked Bilayers of Fully Extended Ceramides with Cholesterol Molecules Associated with the Ceramide Sphingoid Moiety', Journal of Investigative Dermatology, vol. 132, no. 9, pp. 2215-2225. https://doi.org/10.1038/jid.2012.43

APA

Iwai, I., Han, H., den Hollander, L., Svensson, S., Oefverstedt, L-G., Anwar, J., Brewer, J., Bloksgaard, M., Laloeuf, A., Nosek, D., Masich, S., Bagatolli, L. A., Skoglund, U., & Norlen, L. (2012). The Human Skin Barrier Is Organized as Stacked Bilayers of Fully Extended Ceramides with Cholesterol Molecules Associated with the Ceramide Sphingoid Moiety. Journal of Investigative Dermatology, 132(9), 2215-2225. https://doi.org/10.1038/jid.2012.43

Vancouver

Iwai I, Han H, den Hollander L, Svensson S, Oefverstedt L-G, Anwar J et al. The Human Skin Barrier Is Organized as Stacked Bilayers of Fully Extended Ceramides with Cholesterol Molecules Associated with the Ceramide Sphingoid Moiety. Journal of Investigative Dermatology. 2012 Sep;132(9):2215-2225. doi: 10.1038/jid.2012.43

Author

Iwai, Ichiro ; Han, HongMei ; den Hollander, Lianne et al. / The Human Skin Barrier Is Organized as Stacked Bilayers of Fully Extended Ceramides with Cholesterol Molecules Associated with the Ceramide Sphingoid Moiety. In: Journal of Investigative Dermatology. 2012 ; Vol. 132, No. 9. pp. 2215-2225.

Bibtex

@article{caa5bddcb8204cbf8d0d272ced5c4bf6,
title = "The Human Skin Barrier Is Organized as Stacked Bilayers of Fully Extended Ceramides with Cholesterol Molecules Associated with the Ceramide Sphingoid Moiety",
abstract = "The skin barrier is fundamental to terrestrial life and its evolution; it upholds homeostasis and protects against the environment. Skin barrier capacity is controlled by lipids that fill the extracellular space of the skin's surface layer-the stratum corneum. Here we report on the determination of the molecular organization of the skin's lipid matrix in situ, in its near-native state, using a methodological approach combining very high magnification cryo-electron microscopy (EM) of vitreous skin section defocus series, molecular modeling, and EM simulation. The lipids are organized in an arrangement not previously described in a biological system-stacked bilayers of fully extended ceramides (CERs) with cholesterol molecules associated with the CER sphingoid moiety. This arrangement rationalizes the skin's low permeability toward water and toward hydrophilic and lipophilic substances, as well as the skin barrier's robustness toward hydration and dehydration, environmental temperature and pressure changes, stretching, compression, bending, and shearing.",
keywords = "FATTY-ACID, X-RAY-DIFFRACTION, HYDRATION, ELECTRON-MICROSCOPY, PERMEABILITY BARRIER, HUMAN STRATUM-CORNEUM, IN-VIVO, VITREOUS SECTIONS, TRANSBILAYER MOVEMENT, CRYOELECTRON MICROSCOPY",
author = "Ichiro Iwai and HongMei Han and {den Hollander}, Lianne and Stina Svensson and Lars-Goeran Oefverstedt and Jamshed Anwar and Jonathan Brewer and Maria Bloksgaard and Aurelie Laloeuf and Daniel Nosek and Sergej Masich and Bagatolli, {Luis A.} and Ulf Skoglund and Lars Norlen",
year = "2012",
month = sep,
doi = "10.1038/jid.2012.43",
language = "English",
volume = "132",
pages = "2215--2225",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "Nature Publishing Group",
number = "9",

}

RIS

TY - JOUR

T1 - The Human Skin Barrier Is Organized as Stacked Bilayers of Fully Extended Ceramides with Cholesterol Molecules Associated with the Ceramide Sphingoid Moiety

AU - Iwai, Ichiro

AU - Han, HongMei

AU - den Hollander, Lianne

AU - Svensson, Stina

AU - Oefverstedt, Lars-Goeran

AU - Anwar, Jamshed

AU - Brewer, Jonathan

AU - Bloksgaard, Maria

AU - Laloeuf, Aurelie

AU - Nosek, Daniel

AU - Masich, Sergej

AU - Bagatolli, Luis A.

AU - Skoglund, Ulf

AU - Norlen, Lars

PY - 2012/9

Y1 - 2012/9

N2 - The skin barrier is fundamental to terrestrial life and its evolution; it upholds homeostasis and protects against the environment. Skin barrier capacity is controlled by lipids that fill the extracellular space of the skin's surface layer-the stratum corneum. Here we report on the determination of the molecular organization of the skin's lipid matrix in situ, in its near-native state, using a methodological approach combining very high magnification cryo-electron microscopy (EM) of vitreous skin section defocus series, molecular modeling, and EM simulation. The lipids are organized in an arrangement not previously described in a biological system-stacked bilayers of fully extended ceramides (CERs) with cholesterol molecules associated with the CER sphingoid moiety. This arrangement rationalizes the skin's low permeability toward water and toward hydrophilic and lipophilic substances, as well as the skin barrier's robustness toward hydration and dehydration, environmental temperature and pressure changes, stretching, compression, bending, and shearing.

AB - The skin barrier is fundamental to terrestrial life and its evolution; it upholds homeostasis and protects against the environment. Skin barrier capacity is controlled by lipids that fill the extracellular space of the skin's surface layer-the stratum corneum. Here we report on the determination of the molecular organization of the skin's lipid matrix in situ, in its near-native state, using a methodological approach combining very high magnification cryo-electron microscopy (EM) of vitreous skin section defocus series, molecular modeling, and EM simulation. The lipids are organized in an arrangement not previously described in a biological system-stacked bilayers of fully extended ceramides (CERs) with cholesterol molecules associated with the CER sphingoid moiety. This arrangement rationalizes the skin's low permeability toward water and toward hydrophilic and lipophilic substances, as well as the skin barrier's robustness toward hydration and dehydration, environmental temperature and pressure changes, stretching, compression, bending, and shearing.

KW - FATTY-ACID

KW - X-RAY-DIFFRACTION

KW - HYDRATION

KW - ELECTRON-MICROSCOPY

KW - PERMEABILITY BARRIER

KW - HUMAN STRATUM-CORNEUM

KW - IN-VIVO

KW - VITREOUS SECTIONS

KW - TRANSBILAYER MOVEMENT

KW - CRYOELECTRON MICROSCOPY

U2 - 10.1038/jid.2012.43

DO - 10.1038/jid.2012.43

M3 - Journal article

VL - 132

SP - 2215

EP - 2225

JO - Journal of Investigative Dermatology

JF - Journal of Investigative Dermatology

SN - 0022-202X

IS - 9

ER -