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The identification of a species-specific antigen from Necator americanus.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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The identification of a species-specific antigen from Necator americanus. / Pritchard, D. I.; McKean, Paul G.; Rogan, M. T. et al.
In: Parasite Immunology, Vol. 12, No. 3, 05.1990, p. 259-267.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Pritchard, DI, McKean, PG, Rogan, MT & Schad, GA 1990, 'The identification of a species-specific antigen from Necator americanus.', Parasite Immunology, vol. 12, no. 3, pp. 259-267. https://doi.org/10.1111/j.1365-3024.1990.tb00953.x

APA

Pritchard, D. I., McKean, P. G., Rogan, M. T., & Schad, G. A. (1990). The identification of a species-specific antigen from Necator americanus. Parasite Immunology, 12(3), 259-267. https://doi.org/10.1111/j.1365-3024.1990.tb00953.x

Vancouver

Pritchard DI, McKean PG, Rogan MT, Schad GA. The identification of a species-specific antigen from Necator americanus. Parasite Immunology. 1990 May;12(3):259-267. doi: 10.1111/j.1365-3024.1990.tb00953.x

Author

Pritchard, D. I. ; McKean, Paul G. ; Rogan, M. T. et al. / The identification of a species-specific antigen from Necator americanus. In: Parasite Immunology. 1990 ; Vol. 12, No. 3. pp. 259-267.

Bibtex

@article{b6d2836af0d545dca5aee0090bfac3aa,
title = "The identification of a species-specific antigen from Necator americanus.",
abstract = "A 17 kD protein of Necator americanus was isolated by SDS-PAGE and used to raise monospecific antisera in rabbits. ELISA and Western blotting against a range of parasite extracts demonstrated the species specificity of this protein. It is expressed at all stages of the life-cycle, appears to be accumulated through the larval stages to adulthood, and can be localized in the oesophageal glands and cuticle of the adult parasite. The possible nature and diagnostic potential of this protein is discussed.",
keywords = "hookworm • diagnosis • antigen • ELISA • Western blotting • SDS-PAGE • colloidal gold • electron microscopy",
author = "Pritchard, {D. I.} and McKean, {Paul G.} and Rogan, {M. T.} and Schad, {G. A.}",
year = "1990",
month = may,
doi = "10.1111/j.1365-3024.1990.tb00953.x",
language = "English",
volume = "12",
pages = "259--267",
journal = "Parasite Immunology",
issn = "1365-3024",
publisher = "Wiley-Blackwell",
number = "3",

}

RIS

TY - JOUR

T1 - The identification of a species-specific antigen from Necator americanus.

AU - Pritchard, D. I.

AU - McKean, Paul G.

AU - Rogan, M. T.

AU - Schad, G. A.

PY - 1990/5

Y1 - 1990/5

N2 - A 17 kD protein of Necator americanus was isolated by SDS-PAGE and used to raise monospecific antisera in rabbits. ELISA and Western blotting against a range of parasite extracts demonstrated the species specificity of this protein. It is expressed at all stages of the life-cycle, appears to be accumulated through the larval stages to adulthood, and can be localized in the oesophageal glands and cuticle of the adult parasite. The possible nature and diagnostic potential of this protein is discussed.

AB - A 17 kD protein of Necator americanus was isolated by SDS-PAGE and used to raise monospecific antisera in rabbits. ELISA and Western blotting against a range of parasite extracts demonstrated the species specificity of this protein. It is expressed at all stages of the life-cycle, appears to be accumulated through the larval stages to adulthood, and can be localized in the oesophageal glands and cuticle of the adult parasite. The possible nature and diagnostic potential of this protein is discussed.

KW - hookworm • diagnosis • antigen • ELISA • Western blotting • SDS-PAGE • colloidal gold • electron microscopy

U2 - 10.1111/j.1365-3024.1990.tb00953.x

DO - 10.1111/j.1365-3024.1990.tb00953.x

M3 - Journal article

VL - 12

SP - 259

EP - 267

JO - Parasite Immunology

JF - Parasite Immunology

SN - 1365-3024

IS - 3

ER -